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Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

The global demand for food, feed, energy, and water poses extraordinary challenges for future generations. It is evident that robust platforms for the exploration of renewable resources are necessary to overcome these challenges. Within the multinational framework MultiBioPro we are developing biore...

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Autores principales: Ruprecht, Colin, Tohge, Takayuki, Fernie, Alisdair, Mortimer, Cara L., Kozlo, Amanda, Fraser, Paul D., Funke, Norma, Cesarino, Igor, Vanholme, Ruben, Boerjan, Wout, Morreel, Kris, Burgert, Ingo, Gierlinger, Notburga, Bulone, Vincent, Schneider, Vera, Stockero, Andrea, Navarro, Juan Pedro, Pudel, Frank, Tambuyser, Bart, Hygate, James, Bumstead, Jon, Notley, Louis, Persson, Staffan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189316/
https://www.ncbi.nlm.nih.gov/pubmed/24894952
http://dx.doi.org/10.3791/51393
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author Ruprecht, Colin
Tohge, Takayuki
Fernie, Alisdair
Mortimer, Cara L.
Kozlo, Amanda
Fraser, Paul D.
Funke, Norma
Cesarino, Igor
Vanholme, Ruben
Boerjan, Wout
Morreel, Kris
Burgert, Ingo
Gierlinger, Notburga
Bulone, Vincent
Schneider, Vera
Stockero, Andrea
Navarro, Juan Pedro
Pudel, Frank
Tambuyser, Bart
Hygate, James
Bumstead, Jon
Notley, Louis
Persson, Staffan
author_facet Ruprecht, Colin
Tohge, Takayuki
Fernie, Alisdair
Mortimer, Cara L.
Kozlo, Amanda
Fraser, Paul D.
Funke, Norma
Cesarino, Igor
Vanholme, Ruben
Boerjan, Wout
Morreel, Kris
Burgert, Ingo
Gierlinger, Notburga
Bulone, Vincent
Schneider, Vera
Stockero, Andrea
Navarro, Juan Pedro
Pudel, Frank
Tambuyser, Bart
Hygate, James
Bumstead, Jon
Notley, Louis
Persson, Staffan
author_sort Ruprecht, Colin
collection PubMed
description The global demand for food, feed, energy, and water poses extraordinary challenges for future generations. It is evident that robust platforms for the exploration of renewable resources are necessary to overcome these challenges. Within the multinational framework MultiBioPro we are developing biorefinery pipelines to maximize the use of plant biomass. More specifically, we use poplar and tobacco tree (Nicotiana glauca) as target crop species for improving saccharification, isoprenoid, long chain hydrocarbon contents, fiber quality, and suberin and lignin contents. The methods used to obtain these outputs include GC-MS, LC-MS and RNA sequencing platforms. The metabolite pipelines are well established tools to generate these types of data, but also have the limitations in that only well characterized metabolites can be used. The deep sequencing will allow us to include all transcripts present during the developmental stages of the tobacco tree leaf, but has to be mapped back to the sequence of Nicotiana tabacum. With these set-ups, we aim at a basic understanding for underlying processes and at establishing an industrial framework to exploit the outcomes. In a more long term perspective, we believe that data generated here will provide means for a sustainable biorefinery process using poplar and tobacco tree as raw material. To date the basal level of metabolites in the samples have been analyzed and the protocols utilized are provided in this article.
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spelling pubmed-41893162014-10-09 Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry Ruprecht, Colin Tohge, Takayuki Fernie, Alisdair Mortimer, Cara L. Kozlo, Amanda Fraser, Paul D. Funke, Norma Cesarino, Igor Vanholme, Ruben Boerjan, Wout Morreel, Kris Burgert, Ingo Gierlinger, Notburga Bulone, Vincent Schneider, Vera Stockero, Andrea Navarro, Juan Pedro Pudel, Frank Tambuyser, Bart Hygate, James Bumstead, Jon Notley, Louis Persson, Staffan J Vis Exp Environmental Sciences The global demand for food, feed, energy, and water poses extraordinary challenges for future generations. It is evident that robust platforms for the exploration of renewable resources are necessary to overcome these challenges. Within the multinational framework MultiBioPro we are developing biorefinery pipelines to maximize the use of plant biomass. More specifically, we use poplar and tobacco tree (Nicotiana glauca) as target crop species for improving saccharification, isoprenoid, long chain hydrocarbon contents, fiber quality, and suberin and lignin contents. The methods used to obtain these outputs include GC-MS, LC-MS and RNA sequencing platforms. The metabolite pipelines are well established tools to generate these types of data, but also have the limitations in that only well characterized metabolites can be used. The deep sequencing will allow us to include all transcripts present during the developmental stages of the tobacco tree leaf, but has to be mapped back to the sequence of Nicotiana tabacum. With these set-ups, we aim at a basic understanding for underlying processes and at establishing an industrial framework to exploit the outcomes. In a more long term perspective, we believe that data generated here will provide means for a sustainable biorefinery process using poplar and tobacco tree as raw material. To date the basal level of metabolites in the samples have been analyzed and the protocols utilized are provided in this article. MyJove Corporation 2014-05-16 /pmc/articles/PMC4189316/ /pubmed/24894952 http://dx.doi.org/10.3791/51393 Text en Copyright © 2014, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Environmental Sciences
Ruprecht, Colin
Tohge, Takayuki
Fernie, Alisdair
Mortimer, Cara L.
Kozlo, Amanda
Fraser, Paul D.
Funke, Norma
Cesarino, Igor
Vanholme, Ruben
Boerjan, Wout
Morreel, Kris
Burgert, Ingo
Gierlinger, Notburga
Bulone, Vincent
Schneider, Vera
Stockero, Andrea
Navarro, Juan Pedro
Pudel, Frank
Tambuyser, Bart
Hygate, James
Bumstead, Jon
Notley, Louis
Persson, Staffan
Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
title Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
title_full Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
title_fullStr Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
title_full_unstemmed Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
title_short Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry
title_sort transcript and metabolite profiling for the evaluation of tobacco tree and poplar as feedstock for the bio-based industry
topic Environmental Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189316/
https://www.ncbi.nlm.nih.gov/pubmed/24894952
http://dx.doi.org/10.3791/51393
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