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An Isolated Working Heart System for Large Animal Models

Since its introduction in the late 19(th) century, the Langendorff isolated heart perfusion apparatus, and the subsequent development of the working heart model, have been invaluable tools for studying cardiovascular function and disease(1-15). Although the Langendorff heart preparation can be used...

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Autores principales: Schechter, Matthew A., Southerland, Kevin W., Feger, Bryan J., Linder, Dean, Ali, Ayyaz A., Njoroge, Linda, Milano, Carmelo A., Bowles, Dawn E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189428/
https://www.ncbi.nlm.nih.gov/pubmed/24962492
http://dx.doi.org/10.3791/51671
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author Schechter, Matthew A.
Southerland, Kevin W.
Feger, Bryan J.
Linder, Dean
Ali, Ayyaz A.
Njoroge, Linda
Milano, Carmelo A.
Bowles, Dawn E.
author_facet Schechter, Matthew A.
Southerland, Kevin W.
Feger, Bryan J.
Linder, Dean
Ali, Ayyaz A.
Njoroge, Linda
Milano, Carmelo A.
Bowles, Dawn E.
author_sort Schechter, Matthew A.
collection PubMed
description Since its introduction in the late 19(th) century, the Langendorff isolated heart perfusion apparatus, and the subsequent development of the working heart model, have been invaluable tools for studying cardiovascular function and disease(1-15). Although the Langendorff heart preparation can be used for any mammalian heart, most studies involving this apparatus use small animal models (e.g., mouse, rat, and rabbit) due to the increased complexity of systems for larger mammals(1,3,11). One major difficulty is ensuring a constant coronary perfusion pressure over a range of different heart sizes – a key component of any experiment utilizing this device(1,11). By replacing the classic hydrostatic afterload column with a centrifugal pump, the Langendorff working heart apparatus described below allows for easy adjustment and tight regulation of perfusion pressures, meaning the same set-up can be used for various species or heart sizes. Furthermore, this configuration can also seamlessly switch between constant pressure or constant flow during reperfusion, depending on the user’s preferences. The open nature of this setup, despite making temperature regulation more difficult than other designs, allows for easy collection of effluent and ventricular pressure-volume data.
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spelling pubmed-41894282014-10-09 An Isolated Working Heart System for Large Animal Models Schechter, Matthew A. Southerland, Kevin W. Feger, Bryan J. Linder, Dean Ali, Ayyaz A. Njoroge, Linda Milano, Carmelo A. Bowles, Dawn E. J Vis Exp Medicine Since its introduction in the late 19(th) century, the Langendorff isolated heart perfusion apparatus, and the subsequent development of the working heart model, have been invaluable tools for studying cardiovascular function and disease(1-15). Although the Langendorff heart preparation can be used for any mammalian heart, most studies involving this apparatus use small animal models (e.g., mouse, rat, and rabbit) due to the increased complexity of systems for larger mammals(1,3,11). One major difficulty is ensuring a constant coronary perfusion pressure over a range of different heart sizes – a key component of any experiment utilizing this device(1,11). By replacing the classic hydrostatic afterload column with a centrifugal pump, the Langendorff working heart apparatus described below allows for easy adjustment and tight regulation of perfusion pressures, meaning the same set-up can be used for various species or heart sizes. Furthermore, this configuration can also seamlessly switch between constant pressure or constant flow during reperfusion, depending on the user’s preferences. The open nature of this setup, despite making temperature regulation more difficult than other designs, allows for easy collection of effluent and ventricular pressure-volume data. MyJove Corporation 2014-06-11 /pmc/articles/PMC4189428/ /pubmed/24962492 http://dx.doi.org/10.3791/51671 Text en Copyright © 2014, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Medicine
Schechter, Matthew A.
Southerland, Kevin W.
Feger, Bryan J.
Linder, Dean
Ali, Ayyaz A.
Njoroge, Linda
Milano, Carmelo A.
Bowles, Dawn E.
An Isolated Working Heart System for Large Animal Models
title An Isolated Working Heart System for Large Animal Models
title_full An Isolated Working Heart System for Large Animal Models
title_fullStr An Isolated Working Heart System for Large Animal Models
title_full_unstemmed An Isolated Working Heart System for Large Animal Models
title_short An Isolated Working Heart System for Large Animal Models
title_sort isolated working heart system for large animal models
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189428/
https://www.ncbi.nlm.nih.gov/pubmed/24962492
http://dx.doi.org/10.3791/51671
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