Reversible Positioning of Single Molecules inside Zero-Mode Waveguides

[Image: see text] We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to the base of 70 nm diameter...

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Autores principales: Larkin, Joseph, Foquet, Mathieu, Turner, Stephen W., Korlach, Jonas, Wanunu, Meni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189617/
https://www.ncbi.nlm.nih.gov/pubmed/25209321
http://dx.doi.org/10.1021/nl503134x
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author Larkin, Joseph
Foquet, Mathieu
Turner, Stephen W.
Korlach, Jonas
Wanunu, Meni
author_facet Larkin, Joseph
Foquet, Mathieu
Turner, Stephen W.
Korlach, Jonas
Wanunu, Meni
author_sort Larkin, Joseph
collection PubMed
description [Image: see text] We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to the base of 70 nm diameter waveguides for interrogation. Rapid and reversible molecular loading is achieved by controlling the voltage across the device. Using this device we demonstrate protein and DNA loading with efficiency that is orders of magnitude higher than diffusion-based molecular loading.
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spelling pubmed-41896172015-09-11 Reversible Positioning of Single Molecules inside Zero-Mode Waveguides Larkin, Joseph Foquet, Mathieu Turner, Stephen W. Korlach, Jonas Wanunu, Meni Nano Lett [Image: see text] We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to the base of 70 nm diameter waveguides for interrogation. Rapid and reversible molecular loading is achieved by controlling the voltage across the device. Using this device we demonstrate protein and DNA loading with efficiency that is orders of magnitude higher than diffusion-based molecular loading. American Chemical Society 2014-09-11 2014-10-08 /pmc/articles/PMC4189617/ /pubmed/25209321 http://dx.doi.org/10.1021/nl503134x Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Larkin, Joseph
Foquet, Mathieu
Turner, Stephen W.
Korlach, Jonas
Wanunu, Meni
Reversible Positioning of Single Molecules inside Zero-Mode Waveguides
title Reversible Positioning of Single Molecules inside Zero-Mode Waveguides
title_full Reversible Positioning of Single Molecules inside Zero-Mode Waveguides
title_fullStr Reversible Positioning of Single Molecules inside Zero-Mode Waveguides
title_full_unstemmed Reversible Positioning of Single Molecules inside Zero-Mode Waveguides
title_short Reversible Positioning of Single Molecules inside Zero-Mode Waveguides
title_sort reversible positioning of single molecules inside zero-mode waveguides
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189617/
https://www.ncbi.nlm.nih.gov/pubmed/25209321
http://dx.doi.org/10.1021/nl503134x
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AT korlachjonas reversiblepositioningofsinglemoleculesinsidezeromodewaveguides
AT wanunumeni reversiblepositioningofsinglemoleculesinsidezeromodewaveguides

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