Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of Integrin Clustering on Force Transmission

[Image: see text] Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely space...

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Detalles Bibliográficos
Autores principales: Liu, Yang, Medda, Rebecca, Liu, Zheng, Galior, Kornelia, Yehl, Kevin, Spatz, Joachim P., Cavalcanti-Adam, Elisabetta Ada, Salaita, Khalid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189618/
https://www.ncbi.nlm.nih.gov/pubmed/25238229
http://dx.doi.org/10.1021/nl501912g
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author Liu, Yang
Medda, Rebecca
Liu, Zheng
Galior, Kornelia
Yehl, Kevin
Spatz, Joachim P.
Cavalcanti-Adam, Elisabetta Ada
Salaita, Khalid
author_facet Liu, Yang
Medda, Rebecca
Liu, Zheng
Galior, Kornelia
Yehl, Kevin
Spatz, Joachim P.
Cavalcanti-Adam, Elisabetta Ada
Salaita, Khalid
author_sort Liu, Yang
collection PubMed
description [Image: see text] Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can generate a 10-fold fluorescence response to pN-forces. We found that the mechanism of sensing ligand spacing is force-mediated. This strategy is broadly applicable to investigating receptor clustering and its role in mechanotransduction pathways.
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spelling pubmed-41896182015-09-19 Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of Integrin Clustering on Force Transmission Liu, Yang Medda, Rebecca Liu, Zheng Galior, Kornelia Yehl, Kevin Spatz, Joachim P. Cavalcanti-Adam, Elisabetta Ada Salaita, Khalid Nano Lett [Image: see text] Herein we aimed to understand how nanoscale clustering of RGD ligands alters the mechano-regulation of their integrin receptors. We combined molecular tension fluorescence microscopy with block copolymer micelle nanolithography to fabricate substrates with arrays of precisely spaced probes that can generate a 10-fold fluorescence response to pN-forces. We found that the mechanism of sensing ligand spacing is force-mediated. This strategy is broadly applicable to investigating receptor clustering and its role in mechanotransduction pathways. American Chemical Society 2014-09-19 2014-10-08 /pmc/articles/PMC4189618/ /pubmed/25238229 http://dx.doi.org/10.1021/nl501912g Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Liu, Yang
Medda, Rebecca
Liu, Zheng
Galior, Kornelia
Yehl, Kevin
Spatz, Joachim P.
Cavalcanti-Adam, Elisabetta Ada
Salaita, Khalid
Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of Integrin Clustering on Force Transmission
title Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of Integrin Clustering on Force Transmission
title_full Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of Integrin Clustering on Force Transmission
title_fullStr Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of Integrin Clustering on Force Transmission
title_full_unstemmed Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of Integrin Clustering on Force Transmission
title_short Nanoparticle Tension Probes Patterned at the Nanoscale: Impact of Integrin Clustering on Force Transmission
title_sort nanoparticle tension probes patterned at the nanoscale: impact of integrin clustering on force transmission
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189618/
https://www.ncbi.nlm.nih.gov/pubmed/25238229
http://dx.doi.org/10.1021/nl501912g
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