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Contrasting nitrogen fertilization treatments impact xylem gene expression and secondary cell wall lignification in Eucalyptus
BACKGROUND: Nitrogen (N) is a main nutrient required for tree growth and biomass accumulation. In this study, we analyzed the effects of contrasting nitrogen fertilization treatments on the phenotypes of fast growing Eucalyptus hybrids (E. urophylla x E. grandis) with a special focus on xylem second...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189757/ https://www.ncbi.nlm.nih.gov/pubmed/25260963 http://dx.doi.org/10.1186/s12870-014-0256-9 |
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author | Camargo, Eduardo Leal Oliveira Nascimento, Leandro Costa Soler, Marçal Salazar, Marcela Mendes Lepikson-Neto, Jorge Marques, Wesley Leoricy Alves, Ana Teixeira, Paulo José Pereira Lima Mieczkowski, Piotr Carazzolle, Marcelo Falsarella Martinez, Yves Deckmann, Ana Carolina Rodrigues, José Carlos Grima-Pettenati, Jacqueline Pereira, Gonçalo Amarante Guimarães |
author_facet | Camargo, Eduardo Leal Oliveira Nascimento, Leandro Costa Soler, Marçal Salazar, Marcela Mendes Lepikson-Neto, Jorge Marques, Wesley Leoricy Alves, Ana Teixeira, Paulo José Pereira Lima Mieczkowski, Piotr Carazzolle, Marcelo Falsarella Martinez, Yves Deckmann, Ana Carolina Rodrigues, José Carlos Grima-Pettenati, Jacqueline Pereira, Gonçalo Amarante Guimarães |
author_sort | Camargo, Eduardo Leal Oliveira |
collection | PubMed |
description | BACKGROUND: Nitrogen (N) is a main nutrient required for tree growth and biomass accumulation. In this study, we analyzed the effects of contrasting nitrogen fertilization treatments on the phenotypes of fast growing Eucalyptus hybrids (E. urophylla x E. grandis) with a special focus on xylem secondary cell walls and global gene expression patterns. RESULTS: Histological observations of the xylem secondary cell walls further confirmed by chemical analyses showed that lignin was reduced by luxuriant fertilization, whereas a consistent lignin deposition was observed in trees grown in N-limiting conditions. Also, the syringyl/guaiacyl (S/G) ratio was significantly lower in luxuriant nitrogen samples. Deep sequencing RNAseq analyses allowed us to identify a high number of differentially expressed genes (1,469) between contrasting N treatments. This number is dramatically higher than those obtained in similar studies performed in poplar but using microarrays. Remarkably, all the genes involved the general phenylpropanoid metabolism and lignin pathway were found to be down-regulated in response to high N availability. These findings further confirmed by RT-qPCR are in agreement with the reduced amount of lignin in xylem secondary cell walls of these plants. CONCLUSIONS: This work enabled us to identify, at the whole genome level, xylem genes differentially regulated by N availability, some of which are involved in the environmental control of xylogenesis. It further illustrates that N fertilization can be used to alter the quantity and quality of lignocellulosic biomass in Eucalyptus, offering exciting prospects for the pulp and paper industry and for the use of short coppices plantations to produce second generation biofuels. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0256-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4189757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41897572014-10-09 Contrasting nitrogen fertilization treatments impact xylem gene expression and secondary cell wall lignification in Eucalyptus Camargo, Eduardo Leal Oliveira Nascimento, Leandro Costa Soler, Marçal Salazar, Marcela Mendes Lepikson-Neto, Jorge Marques, Wesley Leoricy Alves, Ana Teixeira, Paulo José Pereira Lima Mieczkowski, Piotr Carazzolle, Marcelo Falsarella Martinez, Yves Deckmann, Ana Carolina Rodrigues, José Carlos Grima-Pettenati, Jacqueline Pereira, Gonçalo Amarante Guimarães BMC Plant Biol Research Article BACKGROUND: Nitrogen (N) is a main nutrient required for tree growth and biomass accumulation. In this study, we analyzed the effects of contrasting nitrogen fertilization treatments on the phenotypes of fast growing Eucalyptus hybrids (E. urophylla x E. grandis) with a special focus on xylem secondary cell walls and global gene expression patterns. RESULTS: Histological observations of the xylem secondary cell walls further confirmed by chemical analyses showed that lignin was reduced by luxuriant fertilization, whereas a consistent lignin deposition was observed in trees grown in N-limiting conditions. Also, the syringyl/guaiacyl (S/G) ratio was significantly lower in luxuriant nitrogen samples. Deep sequencing RNAseq analyses allowed us to identify a high number of differentially expressed genes (1,469) between contrasting N treatments. This number is dramatically higher than those obtained in similar studies performed in poplar but using microarrays. Remarkably, all the genes involved the general phenylpropanoid metabolism and lignin pathway were found to be down-regulated in response to high N availability. These findings further confirmed by RT-qPCR are in agreement with the reduced amount of lignin in xylem secondary cell walls of these plants. CONCLUSIONS: This work enabled us to identify, at the whole genome level, xylem genes differentially regulated by N availability, some of which are involved in the environmental control of xylogenesis. It further illustrates that N fertilization can be used to alter the quantity and quality of lignocellulosic biomass in Eucalyptus, offering exciting prospects for the pulp and paper industry and for the use of short coppices plantations to produce second generation biofuels. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12870-014-0256-9) contains supplementary material, which is available to authorized users. BioMed Central 2014-09-28 /pmc/articles/PMC4189757/ /pubmed/25260963 http://dx.doi.org/10.1186/s12870-014-0256-9 Text en © Camargo et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Camargo, Eduardo Leal Oliveira Nascimento, Leandro Costa Soler, Marçal Salazar, Marcela Mendes Lepikson-Neto, Jorge Marques, Wesley Leoricy Alves, Ana Teixeira, Paulo José Pereira Lima Mieczkowski, Piotr Carazzolle, Marcelo Falsarella Martinez, Yves Deckmann, Ana Carolina Rodrigues, José Carlos Grima-Pettenati, Jacqueline Pereira, Gonçalo Amarante Guimarães Contrasting nitrogen fertilization treatments impact xylem gene expression and secondary cell wall lignification in Eucalyptus |
title | Contrasting nitrogen fertilization treatments impact xylem gene expression and secondary cell wall lignification in Eucalyptus |
title_full | Contrasting nitrogen fertilization treatments impact xylem gene expression and secondary cell wall lignification in Eucalyptus |
title_fullStr | Contrasting nitrogen fertilization treatments impact xylem gene expression and secondary cell wall lignification in Eucalyptus |
title_full_unstemmed | Contrasting nitrogen fertilization treatments impact xylem gene expression and secondary cell wall lignification in Eucalyptus |
title_short | Contrasting nitrogen fertilization treatments impact xylem gene expression and secondary cell wall lignification in Eucalyptus |
title_sort | contrasting nitrogen fertilization treatments impact xylem gene expression and secondary cell wall lignification in eucalyptus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189757/ https://www.ncbi.nlm.nih.gov/pubmed/25260963 http://dx.doi.org/10.1186/s12870-014-0256-9 |
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