Cargando…

Evolution of Network Biomarkers from Early to Late Stage Bladder Cancer Samples

We use a systems biology approach to construct protein-protein interaction networks (PPINs) for early and late stage bladder cancer. By comparing the networks of these two stages, we find that both networks showed very significantly different mechanisms. To obtain the differential network structures...

Descripción completa

Detalles Bibliográficos
Autores principales: Wong, Yung-Hao, Li, Cheng-Wei, Chen, Bor-Sen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189772/
https://www.ncbi.nlm.nih.gov/pubmed/25309904
http://dx.doi.org/10.1155/2014/159078
_version_ 1782338419398017024
author Wong, Yung-Hao
Li, Cheng-Wei
Chen, Bor-Sen
author_facet Wong, Yung-Hao
Li, Cheng-Wei
Chen, Bor-Sen
author_sort Wong, Yung-Hao
collection PubMed
description We use a systems biology approach to construct protein-protein interaction networks (PPINs) for early and late stage bladder cancer. By comparing the networks of these two stages, we find that both networks showed very significantly different mechanisms. To obtain the differential network structures between cancer and noncancer PPINs, we constructed cancer PPIN and noncancer PPIN network structures for the two bladder cancer stages using microarray data from cancer cells and their adjacent noncancer cells, respectively. With their carcinogenesis relevance values (CRVs), we identified 152 and 50 significant proteins and their PPI networks (network markers) for early and late stage bladder cancer by statistical assessment. To investigate the evolution of network biomarkers in the carcinogenesis process, primary pathway analysis showed that the significant pathways of early stage bladder cancer are related to ordinary cancer mechanisms, while the ribosome pathway and spliceosome pathway are most important for late stage bladder cancer. Their only intersection is the ubiquitin mediated proteolysis pathway in the whole stage of bladder cancer. The evolution of network biomarkers from early to late stage can reveal the carcinogenesis of bladder cancer. The findings in this study are new clues specific to this study and give us a direction for targeted cancer therapy, and it should be validated in vivo or in vitro in the future.
format Online
Article
Text
id pubmed-4189772
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-41897722014-10-12 Evolution of Network Biomarkers from Early to Late Stage Bladder Cancer Samples Wong, Yung-Hao Li, Cheng-Wei Chen, Bor-Sen Biomed Res Int Research Article We use a systems biology approach to construct protein-protein interaction networks (PPINs) for early and late stage bladder cancer. By comparing the networks of these two stages, we find that both networks showed very significantly different mechanisms. To obtain the differential network structures between cancer and noncancer PPINs, we constructed cancer PPIN and noncancer PPIN network structures for the two bladder cancer stages using microarray data from cancer cells and their adjacent noncancer cells, respectively. With their carcinogenesis relevance values (CRVs), we identified 152 and 50 significant proteins and their PPI networks (network markers) for early and late stage bladder cancer by statistical assessment. To investigate the evolution of network biomarkers in the carcinogenesis process, primary pathway analysis showed that the significant pathways of early stage bladder cancer are related to ordinary cancer mechanisms, while the ribosome pathway and spliceosome pathway are most important for late stage bladder cancer. Their only intersection is the ubiquitin mediated proteolysis pathway in the whole stage of bladder cancer. The evolution of network biomarkers from early to late stage can reveal the carcinogenesis of bladder cancer. The findings in this study are new clues specific to this study and give us a direction for targeted cancer therapy, and it should be validated in vivo or in vitro in the future. Hindawi Publishing Corporation 2014 2014-09-18 /pmc/articles/PMC4189772/ /pubmed/25309904 http://dx.doi.org/10.1155/2014/159078 Text en Copyright © 2014 Yung-Hao Wong et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wong, Yung-Hao
Li, Cheng-Wei
Chen, Bor-Sen
Evolution of Network Biomarkers from Early to Late Stage Bladder Cancer Samples
title Evolution of Network Biomarkers from Early to Late Stage Bladder Cancer Samples
title_full Evolution of Network Biomarkers from Early to Late Stage Bladder Cancer Samples
title_fullStr Evolution of Network Biomarkers from Early to Late Stage Bladder Cancer Samples
title_full_unstemmed Evolution of Network Biomarkers from Early to Late Stage Bladder Cancer Samples
title_short Evolution of Network Biomarkers from Early to Late Stage Bladder Cancer Samples
title_sort evolution of network biomarkers from early to late stage bladder cancer samples
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189772/
https://www.ncbi.nlm.nih.gov/pubmed/25309904
http://dx.doi.org/10.1155/2014/159078
work_keys_str_mv AT wongyunghao evolutionofnetworkbiomarkersfromearlytolatestagebladdercancersamples
AT lichengwei evolutionofnetworkbiomarkersfromearlytolatestagebladdercancersamples
AT chenborsen evolutionofnetworkbiomarkersfromearlytolatestagebladdercancersamples