Cargando…
Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan
OBJECTIVES: To determine the burden and molecular epidemiology of rotavirus gastroenteritis in children hospitalized with severe acute watery diarrhea in Pakistan prior to introduction of rotavirus vaccine. METHODS: A cross-sectional study was carried out over a period of two years from 2006 – 2008...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189951/ https://www.ncbi.nlm.nih.gov/pubmed/25295613 http://dx.doi.org/10.1371/journal.pone.0108221 |
_version_ | 1782338446926282752 |
---|---|
author | Kazi, Abdul Momin Warraich, Gohar Javed Qureshi, Shahida Qureshi, Huma Khan, Muhammad Mubashir Ahmad Zaidi, Anita Kaniz Mehdi |
author_facet | Kazi, Abdul Momin Warraich, Gohar Javed Qureshi, Shahida Qureshi, Huma Khan, Muhammad Mubashir Ahmad Zaidi, Anita Kaniz Mehdi |
author_sort | Kazi, Abdul Momin |
collection | PubMed |
description | OBJECTIVES: To determine the burden and molecular epidemiology of rotavirus gastroenteritis in children hospitalized with severe acute watery diarrhea in Pakistan prior to introduction of rotavirus vaccine. METHODS: A cross-sectional study was carried out over a period of two years from 2006 – 2008 at five sentinel hospitals in the cities of Karachi, Lahore, Rawalpindi, and Peshawar. Stool samples collected from children under five years of age hospitalized with severe acute watery diarrhea were tested for rotavirus antigen via enzyme immunoassay (EIA) (IDEA REF K6020 Oxoid Ltd (Ely), Cambridge, United Kingdom). A subset of EIA positive stool samples were further processed for genotyping. RESULTS: 6679 children were enrolled and stool specimens of 2039 (30.5%) were positive for rotavirus. Rotavirus positivity ranged from 16.3% to 39.4% in the 5 hospitals with highest positivity in Lahore. 1241 (61%) of all rotavirus cases were in infants under one year of age. Among the strains examined for G-serotypes, the occurrence of G1, G2, G9 and G4 strains was found to be 28%, 24%, 14% and 13%, respectively. Among P-types, the most commonly occurring strains were P6 (31.5%) followed by P8 (20%) and P4 (12%). Prevalent rotavirus genotype in hospitalized children of severe diarrhea were G1P[8] 11.6% (69/593), followed by G2P[4] 10.4% (62/593), and G4P[6] 10.1% (60/593). CONCLUSIONS: Approximately one third of children hospitalized with severe gastroenteritis in urban centers in Pakistan have rotavirus. Introduction of rotavirus vaccine in Pakistan's national immunization program could prevent many severe episodes and diarrheal deaths. |
format | Online Article Text |
id | pubmed-4189951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41899512014-10-10 Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan Kazi, Abdul Momin Warraich, Gohar Javed Qureshi, Shahida Qureshi, Huma Khan, Muhammad Mubashir Ahmad Zaidi, Anita Kaniz Mehdi PLoS One Research Article OBJECTIVES: To determine the burden and molecular epidemiology of rotavirus gastroenteritis in children hospitalized with severe acute watery diarrhea in Pakistan prior to introduction of rotavirus vaccine. METHODS: A cross-sectional study was carried out over a period of two years from 2006 – 2008 at five sentinel hospitals in the cities of Karachi, Lahore, Rawalpindi, and Peshawar. Stool samples collected from children under five years of age hospitalized with severe acute watery diarrhea were tested for rotavirus antigen via enzyme immunoassay (EIA) (IDEA REF K6020 Oxoid Ltd (Ely), Cambridge, United Kingdom). A subset of EIA positive stool samples were further processed for genotyping. RESULTS: 6679 children were enrolled and stool specimens of 2039 (30.5%) were positive for rotavirus. Rotavirus positivity ranged from 16.3% to 39.4% in the 5 hospitals with highest positivity in Lahore. 1241 (61%) of all rotavirus cases were in infants under one year of age. Among the strains examined for G-serotypes, the occurrence of G1, G2, G9 and G4 strains was found to be 28%, 24%, 14% and 13%, respectively. Among P-types, the most commonly occurring strains were P6 (31.5%) followed by P8 (20%) and P4 (12%). Prevalent rotavirus genotype in hospitalized children of severe diarrhea were G1P[8] 11.6% (69/593), followed by G2P[4] 10.4% (62/593), and G4P[6] 10.1% (60/593). CONCLUSIONS: Approximately one third of children hospitalized with severe gastroenteritis in urban centers in Pakistan have rotavirus. Introduction of rotavirus vaccine in Pakistan's national immunization program could prevent many severe episodes and diarrheal deaths. Public Library of Science 2014-10-08 /pmc/articles/PMC4189951/ /pubmed/25295613 http://dx.doi.org/10.1371/journal.pone.0108221 Text en © 2014 Kazi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kazi, Abdul Momin Warraich, Gohar Javed Qureshi, Shahida Qureshi, Huma Khan, Muhammad Mubashir Ahmad Zaidi, Anita Kaniz Mehdi Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan |
title | Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan |
title_full | Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan |
title_fullStr | Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan |
title_full_unstemmed | Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan |
title_short | Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan |
title_sort | sentinel hospital-based surveillance for assessment of burden of rotavirus gastroenteritis in children in pakistan |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189951/ https://www.ncbi.nlm.nih.gov/pubmed/25295613 http://dx.doi.org/10.1371/journal.pone.0108221 |
work_keys_str_mv | AT kaziabdulmomin sentinelhospitalbasedsurveillanceforassessmentofburdenofrotavirusgastroenteritisinchildreninpakistan AT warraichgoharjaved sentinelhospitalbasedsurveillanceforassessmentofburdenofrotavirusgastroenteritisinchildreninpakistan AT qureshishahida sentinelhospitalbasedsurveillanceforassessmentofburdenofrotavirusgastroenteritisinchildreninpakistan AT qureshihuma sentinelhospitalbasedsurveillanceforassessmentofburdenofrotavirusgastroenteritisinchildreninpakistan AT khanmuhammadmubashirahmad sentinelhospitalbasedsurveillanceforassessmentofburdenofrotavirusgastroenteritisinchildreninpakistan AT zaidianitakanizmehdi sentinelhospitalbasedsurveillanceforassessmentofburdenofrotavirusgastroenteritisinchildreninpakistan AT sentinelhospitalbasedsurveillanceforassessmentofburdenofrotavirusgastroenteritisinchildreninpakistan |