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Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan

OBJECTIVES: To determine the burden and molecular epidemiology of rotavirus gastroenteritis in children hospitalized with severe acute watery diarrhea in Pakistan prior to introduction of rotavirus vaccine. METHODS: A cross-sectional study was carried out over a period of two years from 2006 – 2008...

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Autores principales: Kazi, Abdul Momin, Warraich, Gohar Javed, Qureshi, Shahida, Qureshi, Huma, Khan, Muhammad Mubashir Ahmad, Zaidi, Anita Kaniz Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189951/
https://www.ncbi.nlm.nih.gov/pubmed/25295613
http://dx.doi.org/10.1371/journal.pone.0108221
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author Kazi, Abdul Momin
Warraich, Gohar Javed
Qureshi, Shahida
Qureshi, Huma
Khan, Muhammad Mubashir Ahmad
Zaidi, Anita Kaniz Mehdi
author_facet Kazi, Abdul Momin
Warraich, Gohar Javed
Qureshi, Shahida
Qureshi, Huma
Khan, Muhammad Mubashir Ahmad
Zaidi, Anita Kaniz Mehdi
author_sort Kazi, Abdul Momin
collection PubMed
description OBJECTIVES: To determine the burden and molecular epidemiology of rotavirus gastroenteritis in children hospitalized with severe acute watery diarrhea in Pakistan prior to introduction of rotavirus vaccine. METHODS: A cross-sectional study was carried out over a period of two years from 2006 – 2008 at five sentinel hospitals in the cities of Karachi, Lahore, Rawalpindi, and Peshawar. Stool samples collected from children under five years of age hospitalized with severe acute watery diarrhea were tested for rotavirus antigen via enzyme immunoassay (EIA) (IDEA REF K6020 Oxoid Ltd (Ely), Cambridge, United Kingdom). A subset of EIA positive stool samples were further processed for genotyping. RESULTS: 6679 children were enrolled and stool specimens of 2039 (30.5%) were positive for rotavirus. Rotavirus positivity ranged from 16.3% to 39.4% in the 5 hospitals with highest positivity in Lahore. 1241 (61%) of all rotavirus cases were in infants under one year of age. Among the strains examined for G-serotypes, the occurrence of G1, G2, G9 and G4 strains was found to be 28%, 24%, 14% and 13%, respectively. Among P-types, the most commonly occurring strains were P6 (31.5%) followed by P8 (20%) and P4 (12%). Prevalent rotavirus genotype in hospitalized children of severe diarrhea were G1P[8] 11.6% (69/593), followed by G2P[4] 10.4% (62/593), and G4P[6] 10.1% (60/593). CONCLUSIONS: Approximately one third of children hospitalized with severe gastroenteritis in urban centers in Pakistan have rotavirus. Introduction of rotavirus vaccine in Pakistan's national immunization program could prevent many severe episodes and diarrheal deaths.
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spelling pubmed-41899512014-10-10 Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan Kazi, Abdul Momin Warraich, Gohar Javed Qureshi, Shahida Qureshi, Huma Khan, Muhammad Mubashir Ahmad Zaidi, Anita Kaniz Mehdi PLoS One Research Article OBJECTIVES: To determine the burden and molecular epidemiology of rotavirus gastroenteritis in children hospitalized with severe acute watery diarrhea in Pakistan prior to introduction of rotavirus vaccine. METHODS: A cross-sectional study was carried out over a period of two years from 2006 – 2008 at five sentinel hospitals in the cities of Karachi, Lahore, Rawalpindi, and Peshawar. Stool samples collected from children under five years of age hospitalized with severe acute watery diarrhea were tested for rotavirus antigen via enzyme immunoassay (EIA) (IDEA REF K6020 Oxoid Ltd (Ely), Cambridge, United Kingdom). A subset of EIA positive stool samples were further processed for genotyping. RESULTS: 6679 children were enrolled and stool specimens of 2039 (30.5%) were positive for rotavirus. Rotavirus positivity ranged from 16.3% to 39.4% in the 5 hospitals with highest positivity in Lahore. 1241 (61%) of all rotavirus cases were in infants under one year of age. Among the strains examined for G-serotypes, the occurrence of G1, G2, G9 and G4 strains was found to be 28%, 24%, 14% and 13%, respectively. Among P-types, the most commonly occurring strains were P6 (31.5%) followed by P8 (20%) and P4 (12%). Prevalent rotavirus genotype in hospitalized children of severe diarrhea were G1P[8] 11.6% (69/593), followed by G2P[4] 10.4% (62/593), and G4P[6] 10.1% (60/593). CONCLUSIONS: Approximately one third of children hospitalized with severe gastroenteritis in urban centers in Pakistan have rotavirus. Introduction of rotavirus vaccine in Pakistan's national immunization program could prevent many severe episodes and diarrheal deaths. Public Library of Science 2014-10-08 /pmc/articles/PMC4189951/ /pubmed/25295613 http://dx.doi.org/10.1371/journal.pone.0108221 Text en © 2014 Kazi et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kazi, Abdul Momin
Warraich, Gohar Javed
Qureshi, Shahida
Qureshi, Huma
Khan, Muhammad Mubashir Ahmad
Zaidi, Anita Kaniz Mehdi
Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan
title Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan
title_full Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan
title_fullStr Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan
title_full_unstemmed Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan
title_short Sentinel Hospital-Based Surveillance for Assessment of Burden of Rotavirus Gastroenteritis in Children in Pakistan
title_sort sentinel hospital-based surveillance for assessment of burden of rotavirus gastroenteritis in children in pakistan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4189951/
https://www.ncbi.nlm.nih.gov/pubmed/25295613
http://dx.doi.org/10.1371/journal.pone.0108221
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