Cargando…
Phenotypic, Ultra-Structural, and Functional Characterization of Bovine Peripheral Blood Dendritic Cell Subsets
Dendritic cells (DC) are multi-functional cells that bridge the gap between innate and adaptive immune systems. In bovine, significant information is lacking on the precise identity and role of peripheral blood DC subsets. In this study, we identify and characterize bovine peripheral blood DC subset...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4190170/ https://www.ncbi.nlm.nih.gov/pubmed/25295753 http://dx.doi.org/10.1371/journal.pone.0109273 |
_version_ | 1782338466528362496 |
---|---|
author | Sei, Janet J. Ochoa, Amanda S. Bishop, Elizabeth Barlow, John W. Golde, William T. |
author_facet | Sei, Janet J. Ochoa, Amanda S. Bishop, Elizabeth Barlow, John W. Golde, William T. |
author_sort | Sei, Janet J. |
collection | PubMed |
description | Dendritic cells (DC) are multi-functional cells that bridge the gap between innate and adaptive immune systems. In bovine, significant information is lacking on the precise identity and role of peripheral blood DC subsets. In this study, we identify and characterize bovine peripheral blood DC subsets directly ex vivo, without further in vitro manipulation. Multi-color flow cytometric analysis revealed that three DC subsets could be identified. Bovine plasmacytoid DC were phenotypically identified by a unique pattern of cell surface protein expression including CD4, exhibited an extensive endoplasmic reticulum and Golgi apparatus, efficiently internalized and degraded exogenous antigen, and were the only peripheral blood cells specialized in the production of type I IFN following activation with Toll-like receptor (TLR) agonists. Conventional DC were identified by expression of a different pattern of cell surface proteins including CD11c, MHC class II, and CD80, among others, the display of extensive dendritic protrusions on their plasma membrane, expression of very high levels of MHC class II and co-stimulatory molecules, efficient internalization and degradation of exogenous antigen, and ready production of detectable levels of TNF-alpha in response to TLR activation. Our investigations also revealed a third novel DC subset that may be a precursor of conventional DC that were MHC class II(+) and CD11c(−). These cells exhibited a smooth plasma membrane with a rounded nucleus, produced TNF-alpha in response to TLR-activation (albeit lower than CD11c(+) DC), and were the least efficient in internalization/degradation of exogenous antigen. These studies define three bovine blood DC subsets with distinct phenotypic and functional characteristics which can be analyzed during immune responses to pathogens and vaccinations of cattle. |
format | Online Article Text |
id | pubmed-4190170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41901702014-10-10 Phenotypic, Ultra-Structural, and Functional Characterization of Bovine Peripheral Blood Dendritic Cell Subsets Sei, Janet J. Ochoa, Amanda S. Bishop, Elizabeth Barlow, John W. Golde, William T. PLoS One Research Article Dendritic cells (DC) are multi-functional cells that bridge the gap between innate and adaptive immune systems. In bovine, significant information is lacking on the precise identity and role of peripheral blood DC subsets. In this study, we identify and characterize bovine peripheral blood DC subsets directly ex vivo, without further in vitro manipulation. Multi-color flow cytometric analysis revealed that three DC subsets could be identified. Bovine plasmacytoid DC were phenotypically identified by a unique pattern of cell surface protein expression including CD4, exhibited an extensive endoplasmic reticulum and Golgi apparatus, efficiently internalized and degraded exogenous antigen, and were the only peripheral blood cells specialized in the production of type I IFN following activation with Toll-like receptor (TLR) agonists. Conventional DC were identified by expression of a different pattern of cell surface proteins including CD11c, MHC class II, and CD80, among others, the display of extensive dendritic protrusions on their plasma membrane, expression of very high levels of MHC class II and co-stimulatory molecules, efficient internalization and degradation of exogenous antigen, and ready production of detectable levels of TNF-alpha in response to TLR activation. Our investigations also revealed a third novel DC subset that may be a precursor of conventional DC that were MHC class II(+) and CD11c(−). These cells exhibited a smooth plasma membrane with a rounded nucleus, produced TNF-alpha in response to TLR-activation (albeit lower than CD11c(+) DC), and were the least efficient in internalization/degradation of exogenous antigen. These studies define three bovine blood DC subsets with distinct phenotypic and functional characteristics which can be analyzed during immune responses to pathogens and vaccinations of cattle. Public Library of Science 2014-10-08 /pmc/articles/PMC4190170/ /pubmed/25295753 http://dx.doi.org/10.1371/journal.pone.0109273 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Sei, Janet J. Ochoa, Amanda S. Bishop, Elizabeth Barlow, John W. Golde, William T. Phenotypic, Ultra-Structural, and Functional Characterization of Bovine Peripheral Blood Dendritic Cell Subsets |
title | Phenotypic, Ultra-Structural, and Functional Characterization of Bovine Peripheral Blood Dendritic Cell Subsets |
title_full | Phenotypic, Ultra-Structural, and Functional Characterization of Bovine Peripheral Blood Dendritic Cell Subsets |
title_fullStr | Phenotypic, Ultra-Structural, and Functional Characterization of Bovine Peripheral Blood Dendritic Cell Subsets |
title_full_unstemmed | Phenotypic, Ultra-Structural, and Functional Characterization of Bovine Peripheral Blood Dendritic Cell Subsets |
title_short | Phenotypic, Ultra-Structural, and Functional Characterization of Bovine Peripheral Blood Dendritic Cell Subsets |
title_sort | phenotypic, ultra-structural, and functional characterization of bovine peripheral blood dendritic cell subsets |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4190170/ https://www.ncbi.nlm.nih.gov/pubmed/25295753 http://dx.doi.org/10.1371/journal.pone.0109273 |
work_keys_str_mv | AT seijanetj phenotypicultrastructuralandfunctionalcharacterizationofbovineperipheralblooddendriticcellsubsets AT ochoaamandas phenotypicultrastructuralandfunctionalcharacterizationofbovineperipheralblooddendriticcellsubsets AT bishopelizabeth phenotypicultrastructuralandfunctionalcharacterizationofbovineperipheralblooddendriticcellsubsets AT barlowjohnw phenotypicultrastructuralandfunctionalcharacterizationofbovineperipheralblooddendriticcellsubsets AT goldewilliamt phenotypicultrastructuralandfunctionalcharacterizationofbovineperipheralblooddendriticcellsubsets |