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iPSC-derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium
We investigated global and regional effects of myocardial transplantation of human induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) in infarcted myocardium. Acute myocardial infarction (MI) was induced by ligation of left coronary artery of severe combined immunodeficient...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4190910/ https://www.ncbi.nlm.nih.gov/pubmed/24974908 http://dx.doi.org/10.1111/jcmm.12351 |
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author | Miao, Qingfeng Shim, Winston Tee, Nicole Lim, Sze Yun Chung, Ying Ying Ja, K P Myu Mia Ooi, Ting Huay Tan, Grace Kong, Geraldine Wei, Heming Lim, Chong Hee Sin, Yoong Kong Wong, Philip |
author_facet | Miao, Qingfeng Shim, Winston Tee, Nicole Lim, Sze Yun Chung, Ying Ying Ja, K P Myu Mia Ooi, Ting Huay Tan, Grace Kong, Geraldine Wei, Heming Lim, Chong Hee Sin, Yoong Kong Wong, Philip |
author_sort | Miao, Qingfeng |
collection | PubMed |
description | We investigated global and regional effects of myocardial transplantation of human induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) in infarcted myocardium. Acute myocardial infarction (MI) was induced by ligation of left coronary artery of severe combined immunodeficient mice before 2 × 10(5) iMSCs or cell-free saline were injected into peri-infarcted anterior free wall. Sham-operated animals received no injection. Global and regional myocardial function was assessed serially at 1-week and 8-week by segmental strain analysis by using two dimensional (2D) speckle tracking echocardiography. Early myocardial remodelling was observed at 1-week and persisted to 8-week with global contractility of ejection fraction and fractional area change in saline- (32.96 ± 14.23%; 21.50 ± 10.07%) and iMSC-injected (32.95 ± 10.31%; 21.00 ± 7.11%) groups significantly depressed as compared to sham control (51.17 ± 11.69%, P < 0.05; 34.86 ± 9.82%, P < 0.05). However, myocardial dilatation was observed in saline-injected animals (4.40 ± 0.62 mm, P < 0.05), but not iMSCs (4.29 ± 0.57 mm), when compared to sham control (3.74 ± 0.32 mm). Furthermore, strain analysis showed significant improved basal anterior wall strain (28.86 ± 8.16%, P < 0.05) in the iMSC group, but not saline-injected (15.81 ± 13.92%), when compared to sham control (22.18 ± 4.13%). This was corroborated by multi-segments deterioration of radial strain only in saline-injected (21.50 ± 5.31%, P < 0.05), but not iMSC (25.67 ± 12.53%), when compared to sham control (34.88 ± 5.77%). Improvements of the myocardial strain coincided with the presence of interconnecting telocytes in interstitial space of the infarcted anterior segment of the heart. Our results show that localized injection of iMSCs alleviates ventricular remodelling, sustains global and regional myocardial strain by paracrine-driven effect on neoangiogenesis and myocardial deformation/compliance via parenchymal and interstitial cell interactions in the infarcted myocardium. |
format | Online Article Text |
id | pubmed-4190910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-41909102014-12-03 iPSC-derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium Miao, Qingfeng Shim, Winston Tee, Nicole Lim, Sze Yun Chung, Ying Ying Ja, K P Myu Mia Ooi, Ting Huay Tan, Grace Kong, Geraldine Wei, Heming Lim, Chong Hee Sin, Yoong Kong Wong, Philip J Cell Mol Med Original Articles We investigated global and regional effects of myocardial transplantation of human induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) in infarcted myocardium. Acute myocardial infarction (MI) was induced by ligation of left coronary artery of severe combined immunodeficient mice before 2 × 10(5) iMSCs or cell-free saline were injected into peri-infarcted anterior free wall. Sham-operated animals received no injection. Global and regional myocardial function was assessed serially at 1-week and 8-week by segmental strain analysis by using two dimensional (2D) speckle tracking echocardiography. Early myocardial remodelling was observed at 1-week and persisted to 8-week with global contractility of ejection fraction and fractional area change in saline- (32.96 ± 14.23%; 21.50 ± 10.07%) and iMSC-injected (32.95 ± 10.31%; 21.00 ± 7.11%) groups significantly depressed as compared to sham control (51.17 ± 11.69%, P < 0.05; 34.86 ± 9.82%, P < 0.05). However, myocardial dilatation was observed in saline-injected animals (4.40 ± 0.62 mm, P < 0.05), but not iMSCs (4.29 ± 0.57 mm), when compared to sham control (3.74 ± 0.32 mm). Furthermore, strain analysis showed significant improved basal anterior wall strain (28.86 ± 8.16%, P < 0.05) in the iMSC group, but not saline-injected (15.81 ± 13.92%), when compared to sham control (22.18 ± 4.13%). This was corroborated by multi-segments deterioration of radial strain only in saline-injected (21.50 ± 5.31%, P < 0.05), but not iMSC (25.67 ± 12.53%), when compared to sham control (34.88 ± 5.77%). Improvements of the myocardial strain coincided with the presence of interconnecting telocytes in interstitial space of the infarcted anterior segment of the heart. Our results show that localized injection of iMSCs alleviates ventricular remodelling, sustains global and regional myocardial strain by paracrine-driven effect on neoangiogenesis and myocardial deformation/compliance via parenchymal and interstitial cell interactions in the infarcted myocardium. Blackwell Publishing Ltd 2014-08 2014-06-28 /pmc/articles/PMC4190910/ /pubmed/24974908 http://dx.doi.org/10.1111/jcmm.12351 Text en © 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Miao, Qingfeng Shim, Winston Tee, Nicole Lim, Sze Yun Chung, Ying Ying Ja, K P Myu Mia Ooi, Ting Huay Tan, Grace Kong, Geraldine Wei, Heming Lim, Chong Hee Sin, Yoong Kong Wong, Philip iPSC-derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium |
title | iPSC-derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium |
title_full | iPSC-derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium |
title_fullStr | iPSC-derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium |
title_full_unstemmed | iPSC-derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium |
title_short | iPSC-derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium |
title_sort | ipsc-derived human mesenchymal stem cells improve myocardial strain of infarcted myocardium |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4190910/ https://www.ncbi.nlm.nih.gov/pubmed/24974908 http://dx.doi.org/10.1111/jcmm.12351 |
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