Cargando…

Absolute and relative quantification of RNA modifications via biosynthetic isotopomers

In the resurging field of RNA modifications, quantification is a bottleneck blocking many exciting avenues. With currently over 150 known nucleoside alterations, detection and quantification methods must encompass multiple modifications for a comprehensive profile. LC–MS/MS approaches offer a perspe...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kellner, Stefanie, Ochel, Antonia, Thüring, Kathrin, Spenkuch, Felix, Neumann, Jennifer, Sharma, Sunny, Entian, Karl-Dieter, Schneider, Dirk, Helm, Mark
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2014
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4191383/ https://www.ncbi.nlm.nih.gov/pubmed/25129236 http://dx.doi.org/10.1093/nar/gku733

Ejemplares similares

Variable presence of 5-methylcytosine in commercial RNA and DNA
por: Schmid, Katharina, et al.
Publicado: (2015)

A fast and efficient translational control system for conditional expression of yeast genes
por: Kötter, Peter, et al.
Publicado: (2009)

EpiChIP: gene-by-gene quantification of epigenetic modification levels
por: Hebenstreit, Daniel, et al.
Publicado: (2011)

Attomole quantification and global profile of RNA modifications: Epitranscriptome of human neural stem cells
por: Basanta-Sanchez, Maria, et al.
Publicado: (2016)

Absolute quantitative measurement of transcriptional kinetic parameters in vivo
por: Iyer, Sukanya, et al.
Publicado: (2016)

Dye label interference with RNA modification reveals 5-fluorouridine as non-covalent inhibitor
por: Spenkuch, Felix, et al.
Publicado: (2014)

Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery
por: Xu, Peng, et al.
Publicado: (2020)

Absolute enrichment: gene set enrichment analysis for homeostatic systems
por: Saxena, Vishal, et al.
Publicado: (2006)

Allele quantification using molecular inversion probes (MIP)
por: Wang, Yuker, et al.
Publicado: (2005)

A deep learning genome-mining strategy for biosynthetic gene cluster prediction
por: Hannigan, Geoffrey D, et al.
Publicado: (2019)

Partial Methylation at Am100 in 18S rRNA of Baker's Yeast Reveals Ribosome Heterogeneity on the Level of Eukaryotic rRNA Modification
por: Buchhaupt, Markus, et al.
Publicado: (2014)

Absolute Quantification of Noncoding RNA by Microscale Thermophoresis
por: Jacob, Dominik, et al.
Publicado: (2019)

Efficient single copy integration via homology-directed repair (scHDR) by 5′modification of large DNA donor fragments in mice
por: Medert, Rebekka, et al.
Publicado: (2022)

Measurement of gluconeogenesis by (2)H(2)O labeling and mass isotopomer distribution analysis
por: Ziari, Naveed, et al.
Publicado: (2023)

A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA
por: Hooker, David J., et al.
Publicado: (2012)

Precise integration of inducible transcriptional elements (PrIITE) enables absolute control of gene expression
por: Pinto, Rita, et al.
Publicado: (2017)

Absolute quantification of proteins in the fatty acid biosynthetic pathway using protein standard absolute quantification
por: Tao, Hui, et al.
Publicado: (2016)

Broadly applicable oligonucleotide mass spectrometry for the analysis of RNA writers and erasers in vitro
por: Hagelskamp, Felix, et al.
Publicado: (2020)

Quantification noise in single cell experiments
por: Reiter, M., et al.
Publicado: (2011)

The exon quantification pipeline (EQP): a comprehensive approach to the quantification of gene, exon and junction expression from RNA-seq data
por: Schuierer, Sven, et al.
Publicado: (2016)

Quantification of siRNA using competitive qPCR
por: Liu, Wei-li, et al.
Publicado: (2009)

Fluorescence strategies for high-throughput quantification of protein interactions
por: Hieb, Aaron R., et al.
Publicado: (2012)

Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
por: Szwagierczak, Aleksandra, et al.
Publicado: (2010)

Quantification of DNA cleavage specificity in Hi-C experiments
por: Meluzzi, Dario, et al.
Publicado: (2016)

psichomics: graphical application for alternative splicing quantification and analysis
por: Saraiva-Agostinho, Nuno, et al.
Publicado: (2019)

DNA methylation determination by liquid chromatography–tandem mass spectrometry using novel biosynthetic [U-(15)N]deoxycytidine and [U-(15)N]methyldeoxycytidine internal standards
por: Quinlivan, Eoin P., et al.
Publicado: (2008)

DNA modification of live cell surface
por: Borisenko, Grigory G., et al.
Publicado: (2009)

In vitro quantification of specific microRNA using molecular beacons
por: Baker, Meredith B., et al.
Publicado: (2012)

Quantification of RNA integrity and its use for measurement of transcript number
por: Brisco, Michael J., et al.
Publicado: (2012)

Easy quantification of template-directed CRISPR/Cas9 editing
por: Brinkman, Eva K, et al.
Publicado: (2018)

Detection and discovery of RNA modifications using microarrays
por: Hiley, Shawna L., et al.
Publicado: (2005)

Real-time quantification of microRNAs by stem–loop RT–PCR
por: Chen, Caifu, et al.
Publicado: (2005)

An efficient method for genome-wide polyadenylation site mapping and RNA quantification
por: Wilkening, Stefan, et al.
Publicado: (2013)

BCseq: accurate single cell RNA-seq quantification with bias correction
por: Chen, Liang, et al.
Publicado: (2018)

Structure-Function Relationship of Substituted Bromomethylcoumarins in Nucleoside Specificity of RNA Alkylation
por: Kellner, Stefanie, et al.
Publicado: (2013)

Receptor-mediated delivery of engineered nucleases for genome modification
por: Chen, Zhong, et al.
Publicado: (2013)

Multiplex cDNA quantification method that facilitates the standardization of gene expression data
por: Gotoh, Osamu, et al.
Publicado: (2011)

Accurate quantification of transcriptome from RNA-Seq data by effective length normalization
por: Lee, Soohyun, et al.
Publicado: (2011)

Information transduction capacity reduces the uncertainties in annotation-free isoform discovery and quantification
por: Deng, Yue, et al.
Publicado: (2017)

Accurate expression quantification from nanopore direct RNA sequencing with NanoCount
por: Gleeson, Josie, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_h8g9mljh8is0j656te7uor5fbp