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Ms1, a novel sRNA interacting with the RNA polymerase core in mycobacteria

Small RNAs (sRNAs) are molecules essential for a number of regulatory processes in the bacterial cell. Here we characterize Ms1, a sRNA that is highly expressed in Mycobacterium smegmatis during stationary phase of growth. By glycerol gradient ultracentrifugation, RNA binding assay, and RNA co-immun...

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Detalles Bibliográficos
Autores principales: Hnilicová, Jarmila, Jirát Matějčková, Jitka, Šiková, Michaela, Pospíšil, Jiří, Halada, Petr, Pánek, Josef, Krásný, Libor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4191392/
https://www.ncbi.nlm.nih.gov/pubmed/25217589
http://dx.doi.org/10.1093/nar/gku793
Descripción
Sumario:Small RNAs (sRNAs) are molecules essential for a number of regulatory processes in the bacterial cell. Here we characterize Ms1, a sRNA that is highly expressed in Mycobacterium smegmatis during stationary phase of growth. By glycerol gradient ultracentrifugation, RNA binding assay, and RNA co-immunoprecipitation, we show that Ms1 interacts with the RNA polymerase (RNAP) core that is free of the primary sigma factor (σ(A)) or any other σ factor. This contrasts with the situation in most other species where it is 6S RNA that interacts with RNAP and this interaction requires the presence of σ(A). The difference in the interaction of the two types of sRNAs (Ms1 or 6S RNA) with RNAP possibly reflects the difference in the composition of the transcriptional machinery between mycobacteria and other species. Unlike Escherichia coli, stationary phase M. smegmatis cells contain relatively few RNAP molecules in complex with σ(A). Thus, Ms1 represents a novel type of small RNAs interacting with RNAP.