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Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development
Proteasomes regulate many essential cellular processes by degrading intracellular proteins. While aging is known to be associated with dysfunction of the proteasome, there are few reports detailing activity and function of proteasomes in the early stages of life. To elucidate the function and develo...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192673/ https://www.ncbi.nlm.nih.gov/pubmed/25177858 http://dx.doi.org/10.3390/biom4030812 |
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author | Claud, Erika C. McDonald, Julie A. K. He, Shu-Mei Yu, Yueyue Duong, Lily Sun, Jun Petrof, Elaine O. |
author_facet | Claud, Erika C. McDonald, Julie A. K. He, Shu-Mei Yu, Yueyue Duong, Lily Sun, Jun Petrof, Elaine O. |
author_sort | Claud, Erika C. |
collection | PubMed |
description | Proteasomes regulate many essential cellular processes by degrading intracellular proteins. While aging is known to be associated with dysfunction of the proteasome, there are few reports detailing activity and function of proteasomes in the early stages of life. To elucidate the function and development of mammalian proteasomes, 26S proteasomes were affinity-purified from rat intestine, spleen and liver. The developmental expression of core, regulatory and immunoproteasome subunits was analyzed by immunoblotting and reverse-transcriptase PCR of mRNA subunits, and proteasome catalytic function was determined by fluorogenic enzymatic assays. The expression of core (β2, β5, α7 and β1) and regulatory (Rpt5) subunits was found to be present at low levels at birth and increased over time particularly at weaning. In contrast, while gradual developmental progression of proteasome structure was also seen with the immunoproteasome subunits (β1i, β5i, and β2i), these were not present at birth. Our studies demonstrate a developmental pattern to 26S proteasome activity and subunit expression, with low levels of core proteasome components and absence of immunoproteasomes at birth followed by increases at later developmental stages. This correlates with findings from other studies of a developmental hyporesponsiveness of the adaptive immune system to allow establishment of microbial colonization immediately after birth. |
format | Online Article Text |
id | pubmed-4192673 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-41926732014-10-10 Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development Claud, Erika C. McDonald, Julie A. K. He, Shu-Mei Yu, Yueyue Duong, Lily Sun, Jun Petrof, Elaine O. Biomolecules Article Proteasomes regulate many essential cellular processes by degrading intracellular proteins. While aging is known to be associated with dysfunction of the proteasome, there are few reports detailing activity and function of proteasomes in the early stages of life. To elucidate the function and development of mammalian proteasomes, 26S proteasomes were affinity-purified from rat intestine, spleen and liver. The developmental expression of core, regulatory and immunoproteasome subunits was analyzed by immunoblotting and reverse-transcriptase PCR of mRNA subunits, and proteasome catalytic function was determined by fluorogenic enzymatic assays. The expression of core (β2, β5, α7 and β1) and regulatory (Rpt5) subunits was found to be present at low levels at birth and increased over time particularly at weaning. In contrast, while gradual developmental progression of proteasome structure was also seen with the immunoproteasome subunits (β1i, β5i, and β2i), these were not present at birth. Our studies demonstrate a developmental pattern to 26S proteasome activity and subunit expression, with low levels of core proteasome components and absence of immunoproteasomes at birth followed by increases at later developmental stages. This correlates with findings from other studies of a developmental hyporesponsiveness of the adaptive immune system to allow establishment of microbial colonization immediately after birth. MDPI 2014-08-29 /pmc/articles/PMC4192673/ /pubmed/25177858 http://dx.doi.org/10.3390/biom4030812 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Claud, Erika C. McDonald, Julie A. K. He, Shu-Mei Yu, Yueyue Duong, Lily Sun, Jun Petrof, Elaine O. Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development |
title | Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development |
title_full | Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development |
title_fullStr | Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development |
title_full_unstemmed | Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development |
title_short | Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development |
title_sort | differential expression of 26s proteasome subunits and functional activity during neonatal development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192673/ https://www.ncbi.nlm.nih.gov/pubmed/25177858 http://dx.doi.org/10.3390/biom4030812 |
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