Cargando…

Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development

Proteasomes regulate many essential cellular processes by degrading intracellular proteins. While aging is known to be associated with dysfunction of the proteasome, there are few reports detailing activity and function of proteasomes in the early stages of life. To elucidate the function and develo...

Descripción completa

Detalles Bibliográficos
Autores principales: Claud, Erika C., McDonald, Julie A. K., He, Shu-Mei, Yu, Yueyue, Duong, Lily, Sun, Jun, Petrof, Elaine O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192673/
https://www.ncbi.nlm.nih.gov/pubmed/25177858
http://dx.doi.org/10.3390/biom4030812
_version_ 1782338823445807104
author Claud, Erika C.
McDonald, Julie A. K.
He, Shu-Mei
Yu, Yueyue
Duong, Lily
Sun, Jun
Petrof, Elaine O.
author_facet Claud, Erika C.
McDonald, Julie A. K.
He, Shu-Mei
Yu, Yueyue
Duong, Lily
Sun, Jun
Petrof, Elaine O.
author_sort Claud, Erika C.
collection PubMed
description Proteasomes regulate many essential cellular processes by degrading intracellular proteins. While aging is known to be associated with dysfunction of the proteasome, there are few reports detailing activity and function of proteasomes in the early stages of life. To elucidate the function and development of mammalian proteasomes, 26S proteasomes were affinity-purified from rat intestine, spleen and liver. The developmental expression of core, regulatory and immunoproteasome subunits was analyzed by immunoblotting and reverse-transcriptase PCR of mRNA subunits, and proteasome catalytic function was determined by fluorogenic enzymatic assays. The expression of core (β2, β5, α7 and β1) and regulatory (Rpt5) subunits was found to be present at low levels at birth and increased over time particularly at weaning. In contrast, while gradual developmental progression of proteasome structure was also seen with the immunoproteasome subunits (β1i, β5i, and β2i), these were not present at birth. Our studies demonstrate a developmental pattern to 26S proteasome activity and subunit expression, with low levels of core proteasome components and absence of immunoproteasomes at birth followed by increases at later developmental stages. This correlates with findings from other studies of a developmental hyporesponsiveness of the adaptive immune system to allow establishment of microbial colonization immediately after birth.
format Online
Article
Text
id pubmed-4192673
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-41926732014-10-10 Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development Claud, Erika C. McDonald, Julie A. K. He, Shu-Mei Yu, Yueyue Duong, Lily Sun, Jun Petrof, Elaine O. Biomolecules Article Proteasomes regulate many essential cellular processes by degrading intracellular proteins. While aging is known to be associated with dysfunction of the proteasome, there are few reports detailing activity and function of proteasomes in the early stages of life. To elucidate the function and development of mammalian proteasomes, 26S proteasomes were affinity-purified from rat intestine, spleen and liver. The developmental expression of core, regulatory and immunoproteasome subunits was analyzed by immunoblotting and reverse-transcriptase PCR of mRNA subunits, and proteasome catalytic function was determined by fluorogenic enzymatic assays. The expression of core (β2, β5, α7 and β1) and regulatory (Rpt5) subunits was found to be present at low levels at birth and increased over time particularly at weaning. In contrast, while gradual developmental progression of proteasome structure was also seen with the immunoproteasome subunits (β1i, β5i, and β2i), these were not present at birth. Our studies demonstrate a developmental pattern to 26S proteasome activity and subunit expression, with low levels of core proteasome components and absence of immunoproteasomes at birth followed by increases at later developmental stages. This correlates with findings from other studies of a developmental hyporesponsiveness of the adaptive immune system to allow establishment of microbial colonization immediately after birth. MDPI 2014-08-29 /pmc/articles/PMC4192673/ /pubmed/25177858 http://dx.doi.org/10.3390/biom4030812 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Claud, Erika C.
McDonald, Julie A. K.
He, Shu-Mei
Yu, Yueyue
Duong, Lily
Sun, Jun
Petrof, Elaine O.
Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development
title Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development
title_full Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development
title_fullStr Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development
title_full_unstemmed Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development
title_short Differential Expression of 26S Proteasome Subunits and Functional Activity during Neonatal Development
title_sort differential expression of 26s proteasome subunits and functional activity during neonatal development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192673/
https://www.ncbi.nlm.nih.gov/pubmed/25177858
http://dx.doi.org/10.3390/biom4030812
work_keys_str_mv AT clauderikac differentialexpressionof26sproteasomesubunitsandfunctionalactivityduringneonataldevelopment
AT mcdonaldjulieak differentialexpressionof26sproteasomesubunitsandfunctionalactivityduringneonataldevelopment
AT heshumei differentialexpressionof26sproteasomesubunitsandfunctionalactivityduringneonataldevelopment
AT yuyueyue differentialexpressionof26sproteasomesubunitsandfunctionalactivityduringneonataldevelopment
AT duonglily differentialexpressionof26sproteasomesubunitsandfunctionalactivityduringneonataldevelopment
AT sunjun differentialexpressionof26sproteasomesubunitsandfunctionalactivityduringneonataldevelopment
AT petrofelaineo differentialexpressionof26sproteasomesubunitsandfunctionalactivityduringneonataldevelopment