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Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury

A preliminary study from our research group showed that picroside II inhibited neuronal apoptosis in ischemic penumbra, reduced ischemic volume, and improved neurobehavioral function in rats with cerebral ischemia. The aim of the present study was to validate the neuroprotective effects of picroside...

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Autores principales: Liu, Guangyi, Zhao, Li, Wang, Tingting, Zhang, Meizeng, Pei, Haitao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192945/
https://www.ncbi.nlm.nih.gov/pubmed/25317155
http://dx.doi.org/10.4103/1673-5374.139460
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author Liu, Guangyi
Zhao, Li
Wang, Tingting
Zhang, Meizeng
Pei, Haitao
author_facet Liu, Guangyi
Zhao, Li
Wang, Tingting
Zhang, Meizeng
Pei, Haitao
author_sort Liu, Guangyi
collection PubMed
description A preliminary study from our research group showed that picroside II inhibited neuronal apoptosis in ischemic penumbra, reduced ischemic volume, and improved neurobehavioral function in rats with cerebral ischemia. The aim of the present study was to validate the neuroprotective effects of picroside II and optimize its therapeutic time window and dose in a rat model of cerebral ischemia. We found that picroside II inhibited cell apoptosis and reduced the expression of neuron-specific enolase, a marker of neuronal damage, in rats after cerebral ischemic injury. The optimal treatment time after ischemic injury and dose were determined, respectively, as follows: (1) 2.0 hours and 10 mg/kg according to the results of toluidine blue staining; (2) 1.5 hours and 10 mg/kg according to early apoptotic ratio by flow cytometry; (3) 2.0 hours and 10 mg/kg according to immunohistochemical and western blot analysis; and (4) 1.5 hours and 10 mg/kg according to reverse transcription polymerase chain reaction. The present findings suggest that an intraperitoneal injection of 10 mg/kg picroside II 1.5–2.0 hours after cerebral ischemic injury in rats is the optimal dose and time for therapeutic benefit.
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spelling pubmed-41929452014-10-14 Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury Liu, Guangyi Zhao, Li Wang, Tingting Zhang, Meizeng Pei, Haitao Neural Regen Res Research and Report A preliminary study from our research group showed that picroside II inhibited neuronal apoptosis in ischemic penumbra, reduced ischemic volume, and improved neurobehavioral function in rats with cerebral ischemia. The aim of the present study was to validate the neuroprotective effects of picroside II and optimize its therapeutic time window and dose in a rat model of cerebral ischemia. We found that picroside II inhibited cell apoptosis and reduced the expression of neuron-specific enolase, a marker of neuronal damage, in rats after cerebral ischemic injury. The optimal treatment time after ischemic injury and dose were determined, respectively, as follows: (1) 2.0 hours and 10 mg/kg according to the results of toluidine blue staining; (2) 1.5 hours and 10 mg/kg according to early apoptotic ratio by flow cytometry; (3) 2.0 hours and 10 mg/kg according to immunohistochemical and western blot analysis; and (4) 1.5 hours and 10 mg/kg according to reverse transcription polymerase chain reaction. The present findings suggest that an intraperitoneal injection of 10 mg/kg picroside II 1.5–2.0 hours after cerebral ischemic injury in rats is the optimal dose and time for therapeutic benefit. Medknow Publications & Media Pvt Ltd 2014-08-01 /pmc/articles/PMC4192945/ /pubmed/25317155 http://dx.doi.org/10.4103/1673-5374.139460 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report
Liu, Guangyi
Zhao, Li
Wang, Tingting
Zhang, Meizeng
Pei, Haitao
Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury
title Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury
title_full Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury
title_fullStr Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury
title_full_unstemmed Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury
title_short Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury
title_sort optimal therapeutic dose and time window of picroside ii in cerebral ischemic injury
topic Research and Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192945/
https://www.ncbi.nlm.nih.gov/pubmed/25317155
http://dx.doi.org/10.4103/1673-5374.139460
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