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Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury
A preliminary study from our research group showed that picroside II inhibited neuronal apoptosis in ischemic penumbra, reduced ischemic volume, and improved neurobehavioral function in rats with cerebral ischemia. The aim of the present study was to validate the neuroprotective effects of picroside...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192945/ https://www.ncbi.nlm.nih.gov/pubmed/25317155 http://dx.doi.org/10.4103/1673-5374.139460 |
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author | Liu, Guangyi Zhao, Li Wang, Tingting Zhang, Meizeng Pei, Haitao |
author_facet | Liu, Guangyi Zhao, Li Wang, Tingting Zhang, Meizeng Pei, Haitao |
author_sort | Liu, Guangyi |
collection | PubMed |
description | A preliminary study from our research group showed that picroside II inhibited neuronal apoptosis in ischemic penumbra, reduced ischemic volume, and improved neurobehavioral function in rats with cerebral ischemia. The aim of the present study was to validate the neuroprotective effects of picroside II and optimize its therapeutic time window and dose in a rat model of cerebral ischemia. We found that picroside II inhibited cell apoptosis and reduced the expression of neuron-specific enolase, a marker of neuronal damage, in rats after cerebral ischemic injury. The optimal treatment time after ischemic injury and dose were determined, respectively, as follows: (1) 2.0 hours and 10 mg/kg according to the results of toluidine blue staining; (2) 1.5 hours and 10 mg/kg according to early apoptotic ratio by flow cytometry; (3) 2.0 hours and 10 mg/kg according to immunohistochemical and western blot analysis; and (4) 1.5 hours and 10 mg/kg according to reverse transcription polymerase chain reaction. The present findings suggest that an intraperitoneal injection of 10 mg/kg picroside II 1.5–2.0 hours after cerebral ischemic injury in rats is the optimal dose and time for therapeutic benefit. |
format | Online Article Text |
id | pubmed-4192945 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-41929452014-10-14 Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury Liu, Guangyi Zhao, Li Wang, Tingting Zhang, Meizeng Pei, Haitao Neural Regen Res Research and Report A preliminary study from our research group showed that picroside II inhibited neuronal apoptosis in ischemic penumbra, reduced ischemic volume, and improved neurobehavioral function in rats with cerebral ischemia. The aim of the present study was to validate the neuroprotective effects of picroside II and optimize its therapeutic time window and dose in a rat model of cerebral ischemia. We found that picroside II inhibited cell apoptosis and reduced the expression of neuron-specific enolase, a marker of neuronal damage, in rats after cerebral ischemic injury. The optimal treatment time after ischemic injury and dose were determined, respectively, as follows: (1) 2.0 hours and 10 mg/kg according to the results of toluidine blue staining; (2) 1.5 hours and 10 mg/kg according to early apoptotic ratio by flow cytometry; (3) 2.0 hours and 10 mg/kg according to immunohistochemical and western blot analysis; and (4) 1.5 hours and 10 mg/kg according to reverse transcription polymerase chain reaction. The present findings suggest that an intraperitoneal injection of 10 mg/kg picroside II 1.5–2.0 hours after cerebral ischemic injury in rats is the optimal dose and time for therapeutic benefit. Medknow Publications & Media Pvt Ltd 2014-08-01 /pmc/articles/PMC4192945/ /pubmed/25317155 http://dx.doi.org/10.4103/1673-5374.139460 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research and Report Liu, Guangyi Zhao, Li Wang, Tingting Zhang, Meizeng Pei, Haitao Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury |
title | Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury |
title_full | Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury |
title_fullStr | Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury |
title_full_unstemmed | Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury |
title_short | Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury |
title_sort | optimal therapeutic dose and time window of picroside ii in cerebral ischemic injury |
topic | Research and Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4192945/ https://www.ncbi.nlm.nih.gov/pubmed/25317155 http://dx.doi.org/10.4103/1673-5374.139460 |
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