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Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments
BACKGROUND: The requirement of a large amount of high-quality RNA is a major limiting factor for microarray experiments using biopsies. An average microarray experiment requires 10–100 μg of RNA. However, due to their small size, most biopsies do not yield this amount. Several different approaches f...
| Autores principales: | , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2004
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC419340/ https://www.ncbi.nlm.nih.gov/pubmed/15119961 http://dx.doi.org/10.1186/1471-2164-5-29 |