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Up-regulation of the chemokine CCL21 in the skin of subjects exposed to irritants

BACKGROUND: Expression of murine CCL21 by dermal lymphatic endothelial cells (LEC) has been demonstrated to be one of the most important steps in Langerhans cell emigration from skin. Previously, our group and others have found that this chemokine is up-regulated in different human inflammatory skin...

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Autores principales: Eberhard, Yanina, Ortiz, Susana, Ruiz Lascano, Alejandro, Kuznitzky, Raquel, Serra, Horacio Marcelo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC419342/
https://www.ncbi.nlm.nih.gov/pubmed/15109401
http://dx.doi.org/10.1186/1471-2172-5-7
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author Eberhard, Yanina
Ortiz, Susana
Ruiz Lascano, Alejandro
Kuznitzky, Raquel
Serra, Horacio Marcelo
author_facet Eberhard, Yanina
Ortiz, Susana
Ruiz Lascano, Alejandro
Kuznitzky, Raquel
Serra, Horacio Marcelo
author_sort Eberhard, Yanina
collection PubMed
description BACKGROUND: Expression of murine CCL21 by dermal lymphatic endothelial cells (LEC) has been demonstrated to be one of the most important steps in Langerhans cell emigration from skin. Previously, our group and others have found that this chemokine is up-regulated in different human inflammatory skin diseases mediated by diverse specific immune responses. This study was carried out to investigate the involvement of CCL21 in human skin after challenge with irritant agents responsible for inducing Irritant Contact Dermatitis (ICD). RESULTS: Eleven normal individuals were challenged with different chemical or physical irritants. Two patients with Allergic Contact Dermatitis (ACD) were also challenged with the relevant antigen in order to have a positive control for CCL21 expression. Macroscopic as well as microscopic responses were evaluated. We observed typical ICD responses with mostly mononuclear cells in perivascular areas, but a predominance of polymorphonuclear cells away from the inflamed blood vessels and in the epidermis at 24 hours. Immunohistochemical studies showed up-regulation of CCL21 by lymphatic endothelial cells in all the biopsies taken from ICD and ACD lesions compared to normal skin. Kinetic study at 10, 48, 96 and 168 hours after contact with a classical irritant (sodium lauryl sulphate) showed that the expression of CCL21 was increased in lymphatic vessels at 10 hours, peaked at 48 hours, and then gradually declined. There was a strong correlation between CCL21 expression and the macroscopic response (r = 0.69; p = 0.0008), but not between CCL21 and the number of infiltrating cells in the lesions. CONCLUSIONS: These results provide new evidence for the role of CCL21 in inflammatory processes. Since the up-regulation of this chemokine was observed in ICD and ACD, it is tempting to speculate that this mechanism operates independently of the type of dermal insult, facilitating the emigration of CCR7+ cells.
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spelling pubmed-4193422004-05-28 Up-regulation of the chemokine CCL21 in the skin of subjects exposed to irritants Eberhard, Yanina Ortiz, Susana Ruiz Lascano, Alejandro Kuznitzky, Raquel Serra, Horacio Marcelo BMC Immunol Research Article BACKGROUND: Expression of murine CCL21 by dermal lymphatic endothelial cells (LEC) has been demonstrated to be one of the most important steps in Langerhans cell emigration from skin. Previously, our group and others have found that this chemokine is up-regulated in different human inflammatory skin diseases mediated by diverse specific immune responses. This study was carried out to investigate the involvement of CCL21 in human skin after challenge with irritant agents responsible for inducing Irritant Contact Dermatitis (ICD). RESULTS: Eleven normal individuals were challenged with different chemical or physical irritants. Two patients with Allergic Contact Dermatitis (ACD) were also challenged with the relevant antigen in order to have a positive control for CCL21 expression. Macroscopic as well as microscopic responses were evaluated. We observed typical ICD responses with mostly mononuclear cells in perivascular areas, but a predominance of polymorphonuclear cells away from the inflamed blood vessels and in the epidermis at 24 hours. Immunohistochemical studies showed up-regulation of CCL21 by lymphatic endothelial cells in all the biopsies taken from ICD and ACD lesions compared to normal skin. Kinetic study at 10, 48, 96 and 168 hours after contact with a classical irritant (sodium lauryl sulphate) showed that the expression of CCL21 was increased in lymphatic vessels at 10 hours, peaked at 48 hours, and then gradually declined. There was a strong correlation between CCL21 expression and the macroscopic response (r = 0.69; p = 0.0008), but not between CCL21 and the number of infiltrating cells in the lesions. CONCLUSIONS: These results provide new evidence for the role of CCL21 in inflammatory processes. Since the up-regulation of this chemokine was observed in ICD and ACD, it is tempting to speculate that this mechanism operates independently of the type of dermal insult, facilitating the emigration of CCR7+ cells. BioMed Central 2004-04-26 /pmc/articles/PMC419342/ /pubmed/15109401 http://dx.doi.org/10.1186/1471-2172-5-7 Text en Copyright © 2004 Eberhard et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Eberhard, Yanina
Ortiz, Susana
Ruiz Lascano, Alejandro
Kuznitzky, Raquel
Serra, Horacio Marcelo
Up-regulation of the chemokine CCL21 in the skin of subjects exposed to irritants
title Up-regulation of the chemokine CCL21 in the skin of subjects exposed to irritants
title_full Up-regulation of the chemokine CCL21 in the skin of subjects exposed to irritants
title_fullStr Up-regulation of the chemokine CCL21 in the skin of subjects exposed to irritants
title_full_unstemmed Up-regulation of the chemokine CCL21 in the skin of subjects exposed to irritants
title_short Up-regulation of the chemokine CCL21 in the skin of subjects exposed to irritants
title_sort up-regulation of the chemokine ccl21 in the skin of subjects exposed to irritants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC419342/
https://www.ncbi.nlm.nih.gov/pubmed/15109401
http://dx.doi.org/10.1186/1471-2172-5-7
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