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Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system

Measurement of the correlation between sensor-protein expression, motility and environmental change is important for understanding the adaptation process of cells during their change of generation. We have developed a novel assay exploiting the on-chip cultivation system, which enabled us to observe...

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Detalles Bibliográficos
Autores principales: Inoue, Ippei, Shiomi, Daisuke, Kawagishi, Ikuro, Yasuda, Kenji
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC419370/
https://www.ncbi.nlm.nih.gov/pubmed/15119953
http://dx.doi.org/10.1186/1477-3155-2-4
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author Inoue, Ippei
Shiomi, Daisuke
Kawagishi, Ikuro
Yasuda, Kenji
author_facet Inoue, Ippei
Shiomi, Daisuke
Kawagishi, Ikuro
Yasuda, Kenji
author_sort Inoue, Ippei
collection PubMed
description Measurement of the correlation between sensor-protein expression, motility and environmental change is important for understanding the adaptation process of cells during their change of generation. We have developed a novel assay exploiting the on-chip cultivation system, which enabled us to observe the change of the localization of expressed sensor-protein and the motility for generations. Localization of the aspartate sensitive sensor protein at two poles in Escherichia coli decreased quickly after the aspartate was added into the cultivation medium. However, it took more than three generations for recovering the localization after the removal of aspartate from the medium. Moreover, the tumbling frequency was strongly related to the localization of the sensor protein in a cell. The results indicate that the change of the spatial localization of sensor protein, which was inherited for more than three generations, may contribute to cells, motility as the inheritable information.
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spelling pubmed-4193702004-05-28 Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system Inoue, Ippei Shiomi, Daisuke Kawagishi, Ikuro Yasuda, Kenji J Nanobiotechnology Short Communication Measurement of the correlation between sensor-protein expression, motility and environmental change is important for understanding the adaptation process of cells during their change of generation. We have developed a novel assay exploiting the on-chip cultivation system, which enabled us to observe the change of the localization of expressed sensor-protein and the motility for generations. Localization of the aspartate sensitive sensor protein at two poles in Escherichia coli decreased quickly after the aspartate was added into the cultivation medium. However, it took more than three generations for recovering the localization after the removal of aspartate from the medium. Moreover, the tumbling frequency was strongly related to the localization of the sensor protein in a cell. The results indicate that the change of the spatial localization of sensor protein, which was inherited for more than three generations, may contribute to cells, motility as the inheritable information. BioMed Central 2004-04-30 /pmc/articles/PMC419370/ /pubmed/15119953 http://dx.doi.org/10.1186/1477-3155-2-4 Text en Copyright © 2004 Inoue et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Short Communication
Inoue, Ippei
Shiomi, Daisuke
Kawagishi, Ikuro
Yasuda, Kenji
Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system
title Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system
title_full Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system
title_fullStr Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system
title_full_unstemmed Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system
title_short Simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system
title_sort simultaneous measurement of sensor-protein dynamics and motility of a single cell by on-chip microcultivation system
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC419370/
https://www.ncbi.nlm.nih.gov/pubmed/15119953
http://dx.doi.org/10.1186/1477-3155-2-4
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