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Dual Effects of Alpha-Arbutin on Monophenolase and Diphenolase Activities of Mushroom Tyrosinase

The effects of α-arbutin on the monophenolase and diphenolase activities of mushroom tyrosinase were investigated. The results showed that α-arbutin inhibited monophenolase activity but it activated diphenolase activity. For monophenolase activity, IC(50) value was 4.5 mmol·L(−1) and 4.18 mmol·L(−1)...

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Detalles Bibliográficos
Autores principales: Qin, Liang, Wu, Yang, Liu, Youting, Chen, Yiming, Zhang, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4193772/
https://www.ncbi.nlm.nih.gov/pubmed/25303458
http://dx.doi.org/10.1371/journal.pone.0109398
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author Qin, Liang
Wu, Yang
Liu, Youting
Chen, Yiming
Zhang, Peng
author_facet Qin, Liang
Wu, Yang
Liu, Youting
Chen, Yiming
Zhang, Peng
author_sort Qin, Liang
collection PubMed
description The effects of α-arbutin on the monophenolase and diphenolase activities of mushroom tyrosinase were investigated. The results showed that α-arbutin inhibited monophenolase activity but it activated diphenolase activity. For monophenolase activity, IC(50) value was 4.5 mmol·L(−1) and 4.18 mmol·L(−1) of α-arbutin could extend the lag time from 40.5 s to 167.3 s. Alpha- arbutin is proposed to be regarded as a triphenolic substrate by the enzyme during catalyzation, leading to the suicide inactivation of the active site of tyrosinase. For diphenolase activity, α-arbutin acted as an activator and its activation mechanism was mixed type activation. To reveal such activation, it should be mainly refered to the conformational changes in tyrosinase caused by the interaction of α-arbutin with residues located at the entrance to the active site, and the decrease of the effect of suicide inactivation.
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spelling pubmed-41937722014-10-14 Dual Effects of Alpha-Arbutin on Monophenolase and Diphenolase Activities of Mushroom Tyrosinase Qin, Liang Wu, Yang Liu, Youting Chen, Yiming Zhang, Peng PLoS One Research Article The effects of α-arbutin on the monophenolase and diphenolase activities of mushroom tyrosinase were investigated. The results showed that α-arbutin inhibited monophenolase activity but it activated diphenolase activity. For monophenolase activity, IC(50) value was 4.5 mmol·L(−1) and 4.18 mmol·L(−1) of α-arbutin could extend the lag time from 40.5 s to 167.3 s. Alpha- arbutin is proposed to be regarded as a triphenolic substrate by the enzyme during catalyzation, leading to the suicide inactivation of the active site of tyrosinase. For diphenolase activity, α-arbutin acted as an activator and its activation mechanism was mixed type activation. To reveal such activation, it should be mainly refered to the conformational changes in tyrosinase caused by the interaction of α-arbutin with residues located at the entrance to the active site, and the decrease of the effect of suicide inactivation. Public Library of Science 2014-10-10 /pmc/articles/PMC4193772/ /pubmed/25303458 http://dx.doi.org/10.1371/journal.pone.0109398 Text en © 2014 Qin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Qin, Liang
Wu, Yang
Liu, Youting
Chen, Yiming
Zhang, Peng
Dual Effects of Alpha-Arbutin on Monophenolase and Diphenolase Activities of Mushroom Tyrosinase
title Dual Effects of Alpha-Arbutin on Monophenolase and Diphenolase Activities of Mushroom Tyrosinase
title_full Dual Effects of Alpha-Arbutin on Monophenolase and Diphenolase Activities of Mushroom Tyrosinase
title_fullStr Dual Effects of Alpha-Arbutin on Monophenolase and Diphenolase Activities of Mushroom Tyrosinase
title_full_unstemmed Dual Effects of Alpha-Arbutin on Monophenolase and Diphenolase Activities of Mushroom Tyrosinase
title_short Dual Effects of Alpha-Arbutin on Monophenolase and Diphenolase Activities of Mushroom Tyrosinase
title_sort dual effects of alpha-arbutin on monophenolase and diphenolase activities of mushroom tyrosinase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4193772/
https://www.ncbi.nlm.nih.gov/pubmed/25303458
http://dx.doi.org/10.1371/journal.pone.0109398
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