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IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease
OBJECTIVE: IgG4-related disease is an emerging clinical entity which frequently involves tissue within the orbit. In order to appreciate the implications of IgG4 immunostaining, we analyzed gene expression and the prevalence of IgG4- immunostaining among subjects with orbital inflammatory diseases....
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4193851/ https://www.ncbi.nlm.nih.gov/pubmed/25303270 http://dx.doi.org/10.1371/journal.pone.0109847 |
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author | Wong, Amanda J. Planck, Stephen R. Choi, Dongseok Harrington, Christina A. Troxell, Megan L. Houghton, Donald C. Stauffer, Patrick Wilson, David J. Grossniklaus, Hans E. Dailey, Roger A. Ng, John D. Steele, Eric A. Harris, Gerald J. Czyz, Craig Foster, Jill A. White, Valerie A. Dolman, Peter J. Kazim, Michael Patel, Payal J. Edward, Deepak P. Katan, Hind al Hussain, Hailah al Selva, Dinesh Yeatts, R. Patrick Korn, Bobby S. Kikkawa, Don O. Rosenbaum, James T. |
author_facet | Wong, Amanda J. Planck, Stephen R. Choi, Dongseok Harrington, Christina A. Troxell, Megan L. Houghton, Donald C. Stauffer, Patrick Wilson, David J. Grossniklaus, Hans E. Dailey, Roger A. Ng, John D. Steele, Eric A. Harris, Gerald J. Czyz, Craig Foster, Jill A. White, Valerie A. Dolman, Peter J. Kazim, Michael Patel, Payal J. Edward, Deepak P. Katan, Hind al Hussain, Hailah al Selva, Dinesh Yeatts, R. Patrick Korn, Bobby S. Kikkawa, Don O. Rosenbaum, James T. |
author_sort | Wong, Amanda J. |
collection | PubMed |
description | OBJECTIVE: IgG4-related disease is an emerging clinical entity which frequently involves tissue within the orbit. In order to appreciate the implications of IgG4 immunostaining, we analyzed gene expression and the prevalence of IgG4- immunostaining among subjects with orbital inflammatory diseases. METHODS: We organized an international consortium to collect orbital biopsies from 108 subjects including 22 with no known orbital disease, 42 with nonspecific orbital inflammatory disease (NSOI), 26 with thyroid eye disease (TED), 12 with sarcoidosis, and 6 with granulomatosis with polyangiitis (GPA). Lacrimal gland and orbital adipose tissue biopsies were immunostained for IgG4 or IgG secreting plasma cells. RNA transcripts were quantified by Affymetrix arrays. RESULTS: None of the healthy controls or subjects with TED had substantial IgG4 staining. Among the 63 others, the prevalence of significant IgG4-immunostaining ranged from 11 to 39% depending on the definition for significant. IgG4 staining was detectable in the majority of tissues from subjects with GPA and less commonly in tissue from subjects with sarcoidosis or NSOI. The detection of IgG4+ cells correlated with inflammation in the lacrimal gland based on histology. IgG4 staining tissue expressed an increase in transcripts associated with inflammation, especially B cell-related genes. Functional annotation analysis confirmed this. CONCLUSION: IgG4+ plasma cells are common in orbital tissue from patients with sarcoidosis, GPA, or NSOI. Even using the low threshold of 10 IgG4+ cells/high powered field, IgG4 staining correlates with increased inflammation in the lacrimal gland based on histology and gene expression. |
format | Online Article Text |
id | pubmed-4193851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41938512014-10-14 IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease Wong, Amanda J. Planck, Stephen R. Choi, Dongseok Harrington, Christina A. Troxell, Megan L. Houghton, Donald C. Stauffer, Patrick Wilson, David J. Grossniklaus, Hans E. Dailey, Roger A. Ng, John D. Steele, Eric A. Harris, Gerald J. Czyz, Craig Foster, Jill A. White, Valerie A. Dolman, Peter J. Kazim, Michael Patel, Payal J. Edward, Deepak P. Katan, Hind al Hussain, Hailah al Selva, Dinesh Yeatts, R. Patrick Korn, Bobby S. Kikkawa, Don O. Rosenbaum, James T. PLoS One Research Article OBJECTIVE: IgG4-related disease is an emerging clinical entity which frequently involves tissue within the orbit. In order to appreciate the implications of IgG4 immunostaining, we analyzed gene expression and the prevalence of IgG4- immunostaining among subjects with orbital inflammatory diseases. METHODS: We organized an international consortium to collect orbital biopsies from 108 subjects including 22 with no known orbital disease, 42 with nonspecific orbital inflammatory disease (NSOI), 26 with thyroid eye disease (TED), 12 with sarcoidosis, and 6 with granulomatosis with polyangiitis (GPA). Lacrimal gland and orbital adipose tissue biopsies were immunostained for IgG4 or IgG secreting plasma cells. RNA transcripts were quantified by Affymetrix arrays. RESULTS: None of the healthy controls or subjects with TED had substantial IgG4 staining. Among the 63 others, the prevalence of significant IgG4-immunostaining ranged from 11 to 39% depending on the definition for significant. IgG4 staining was detectable in the majority of tissues from subjects with GPA and less commonly in tissue from subjects with sarcoidosis or NSOI. The detection of IgG4+ cells correlated with inflammation in the lacrimal gland based on histology. IgG4 staining tissue expressed an increase in transcripts associated with inflammation, especially B cell-related genes. Functional annotation analysis confirmed this. CONCLUSION: IgG4+ plasma cells are common in orbital tissue from patients with sarcoidosis, GPA, or NSOI. Even using the low threshold of 10 IgG4+ cells/high powered field, IgG4 staining correlates with increased inflammation in the lacrimal gland based on histology and gene expression. Public Library of Science 2014-10-10 /pmc/articles/PMC4193851/ /pubmed/25303270 http://dx.doi.org/10.1371/journal.pone.0109847 Text en © 2014 Wong et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wong, Amanda J. Planck, Stephen R. Choi, Dongseok Harrington, Christina A. Troxell, Megan L. Houghton, Donald C. Stauffer, Patrick Wilson, David J. Grossniklaus, Hans E. Dailey, Roger A. Ng, John D. Steele, Eric A. Harris, Gerald J. Czyz, Craig Foster, Jill A. White, Valerie A. Dolman, Peter J. Kazim, Michael Patel, Payal J. Edward, Deepak P. Katan, Hind al Hussain, Hailah al Selva, Dinesh Yeatts, R. Patrick Korn, Bobby S. Kikkawa, Don O. Rosenbaum, James T. IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease |
title | IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease |
title_full | IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease |
title_fullStr | IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease |
title_full_unstemmed | IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease |
title_short | IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease |
title_sort | igg4 immunostaining and its implications in orbital inflammatory disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4193851/ https://www.ncbi.nlm.nih.gov/pubmed/25303270 http://dx.doi.org/10.1371/journal.pone.0109847 |
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