Cargando…

IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease

OBJECTIVE: IgG4-related disease is an emerging clinical entity which frequently involves tissue within the orbit. In order to appreciate the implications of IgG4 immunostaining, we analyzed gene expression and the prevalence of IgG4- immunostaining among subjects with orbital inflammatory diseases....

Descripción completa

Detalles Bibliográficos
Autores principales: Wong, Amanda J., Planck, Stephen R., Choi, Dongseok, Harrington, Christina A., Troxell, Megan L., Houghton, Donald C., Stauffer, Patrick, Wilson, David J., Grossniklaus, Hans E., Dailey, Roger A., Ng, John D., Steele, Eric A., Harris, Gerald J., Czyz, Craig, Foster, Jill A., White, Valerie A., Dolman, Peter J., Kazim, Michael, Patel, Payal J., Edward, Deepak P., Katan, Hind al, Hussain, Hailah al, Selva, Dinesh, Yeatts, R. Patrick, Korn, Bobby S., Kikkawa, Don O., Rosenbaum, James T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4193851/
https://www.ncbi.nlm.nih.gov/pubmed/25303270
http://dx.doi.org/10.1371/journal.pone.0109847
_version_ 1782339044668080128
author Wong, Amanda J.
Planck, Stephen R.
Choi, Dongseok
Harrington, Christina A.
Troxell, Megan L.
Houghton, Donald C.
Stauffer, Patrick
Wilson, David J.
Grossniklaus, Hans E.
Dailey, Roger A.
Ng, John D.
Steele, Eric A.
Harris, Gerald J.
Czyz, Craig
Foster, Jill A.
White, Valerie A.
Dolman, Peter J.
Kazim, Michael
Patel, Payal J.
Edward, Deepak P.
Katan, Hind al
Hussain, Hailah al
Selva, Dinesh
Yeatts, R. Patrick
Korn, Bobby S.
Kikkawa, Don O.
Rosenbaum, James T.
author_facet Wong, Amanda J.
Planck, Stephen R.
Choi, Dongseok
Harrington, Christina A.
Troxell, Megan L.
Houghton, Donald C.
Stauffer, Patrick
Wilson, David J.
Grossniklaus, Hans E.
Dailey, Roger A.
Ng, John D.
Steele, Eric A.
Harris, Gerald J.
Czyz, Craig
Foster, Jill A.
White, Valerie A.
Dolman, Peter J.
Kazim, Michael
Patel, Payal J.
Edward, Deepak P.
Katan, Hind al
Hussain, Hailah al
Selva, Dinesh
Yeatts, R. Patrick
Korn, Bobby S.
Kikkawa, Don O.
Rosenbaum, James T.
author_sort Wong, Amanda J.
collection PubMed
description OBJECTIVE: IgG4-related disease is an emerging clinical entity which frequently involves tissue within the orbit. In order to appreciate the implications of IgG4 immunostaining, we analyzed gene expression and the prevalence of IgG4- immunostaining among subjects with orbital inflammatory diseases. METHODS: We organized an international consortium to collect orbital biopsies from 108 subjects including 22 with no known orbital disease, 42 with nonspecific orbital inflammatory disease (NSOI), 26 with thyroid eye disease (TED), 12 with sarcoidosis, and 6 with granulomatosis with polyangiitis (GPA). Lacrimal gland and orbital adipose tissue biopsies were immunostained for IgG4 or IgG secreting plasma cells. RNA transcripts were quantified by Affymetrix arrays. RESULTS: None of the healthy controls or subjects with TED had substantial IgG4 staining. Among the 63 others, the prevalence of significant IgG4-immunostaining ranged from 11 to 39% depending on the definition for significant. IgG4 staining was detectable in the majority of tissues from subjects with GPA and less commonly in tissue from subjects with sarcoidosis or NSOI. The detection of IgG4+ cells correlated with inflammation in the lacrimal gland based on histology. IgG4 staining tissue expressed an increase in transcripts associated with inflammation, especially B cell-related genes. Functional annotation analysis confirmed this. CONCLUSION: IgG4+ plasma cells are common in orbital tissue from patients with sarcoidosis, GPA, or NSOI. Even using the low threshold of 10 IgG4+ cells/high powered field, IgG4 staining correlates with increased inflammation in the lacrimal gland based on histology and gene expression.
format Online
Article
Text
id pubmed-4193851
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-41938512014-10-14 IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease Wong, Amanda J. Planck, Stephen R. Choi, Dongseok Harrington, Christina A. Troxell, Megan L. Houghton, Donald C. Stauffer, Patrick Wilson, David J. Grossniklaus, Hans E. Dailey, Roger A. Ng, John D. Steele, Eric A. Harris, Gerald J. Czyz, Craig Foster, Jill A. White, Valerie A. Dolman, Peter J. Kazim, Michael Patel, Payal J. Edward, Deepak P. Katan, Hind al Hussain, Hailah al Selva, Dinesh Yeatts, R. Patrick Korn, Bobby S. Kikkawa, Don O. Rosenbaum, James T. PLoS One Research Article OBJECTIVE: IgG4-related disease is an emerging clinical entity which frequently involves tissue within the orbit. In order to appreciate the implications of IgG4 immunostaining, we analyzed gene expression and the prevalence of IgG4- immunostaining among subjects with orbital inflammatory diseases. METHODS: We organized an international consortium to collect orbital biopsies from 108 subjects including 22 with no known orbital disease, 42 with nonspecific orbital inflammatory disease (NSOI), 26 with thyroid eye disease (TED), 12 with sarcoidosis, and 6 with granulomatosis with polyangiitis (GPA). Lacrimal gland and orbital adipose tissue biopsies were immunostained for IgG4 or IgG secreting plasma cells. RNA transcripts were quantified by Affymetrix arrays. RESULTS: None of the healthy controls or subjects with TED had substantial IgG4 staining. Among the 63 others, the prevalence of significant IgG4-immunostaining ranged from 11 to 39% depending on the definition for significant. IgG4 staining was detectable in the majority of tissues from subjects with GPA and less commonly in tissue from subjects with sarcoidosis or NSOI. The detection of IgG4+ cells correlated with inflammation in the lacrimal gland based on histology. IgG4 staining tissue expressed an increase in transcripts associated with inflammation, especially B cell-related genes. Functional annotation analysis confirmed this. CONCLUSION: IgG4+ plasma cells are common in orbital tissue from patients with sarcoidosis, GPA, or NSOI. Even using the low threshold of 10 IgG4+ cells/high powered field, IgG4 staining correlates with increased inflammation in the lacrimal gland based on histology and gene expression. Public Library of Science 2014-10-10 /pmc/articles/PMC4193851/ /pubmed/25303270 http://dx.doi.org/10.1371/journal.pone.0109847 Text en © 2014 Wong et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wong, Amanda J.
Planck, Stephen R.
Choi, Dongseok
Harrington, Christina A.
Troxell, Megan L.
Houghton, Donald C.
Stauffer, Patrick
Wilson, David J.
Grossniklaus, Hans E.
Dailey, Roger A.
Ng, John D.
Steele, Eric A.
Harris, Gerald J.
Czyz, Craig
Foster, Jill A.
White, Valerie A.
Dolman, Peter J.
Kazim, Michael
Patel, Payal J.
Edward, Deepak P.
Katan, Hind al
Hussain, Hailah al
Selva, Dinesh
Yeatts, R. Patrick
Korn, Bobby S.
Kikkawa, Don O.
Rosenbaum, James T.
IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease
title IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease
title_full IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease
title_fullStr IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease
title_full_unstemmed IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease
title_short IgG4 Immunostaining and Its Implications in Orbital Inflammatory Disease
title_sort igg4 immunostaining and its implications in orbital inflammatory disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4193851/
https://www.ncbi.nlm.nih.gov/pubmed/25303270
http://dx.doi.org/10.1371/journal.pone.0109847
work_keys_str_mv AT wongamandaj igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT planckstephenr igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT choidongseok igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT harringtonchristinaa igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT troxellmeganl igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT houghtondonaldc igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT staufferpatrick igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT wilsondavidj igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT grossniklaushanse igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT daileyrogera igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT ngjohnd igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT steeleerica igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT harrisgeraldj igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT czyzcraig igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT fosterjilla igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT whitevaleriea igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT dolmanpeterj igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT kazimmichael igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT patelpayalj igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT edwarddeepakp igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT katanhindal igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT hussainhailahal igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT selvadinesh igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT yeattsrpatrick igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT kornbobbys igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT kikkawadono igg4immunostaininganditsimplicationsinorbitalinflammatorydisease
AT rosenbaumjamest igg4immunostaininganditsimplicationsinorbitalinflammatorydisease