Cargando…

A Novel System for Simultaneous or Sequential Integration of Multiple Gene-Loading Vectors into a Defined Site of a Human Artificial Chromosome

Human artificial chromosomes (HACs) are gene-delivery vectors suitable for introducing large DNA fragments into mammalian cells. Although a HAC theoretically incorporates multiple gene expression cassettes of unlimited DNA size, its application has been limited because the conventional gene-loading...

Descripción completa

Detalles Bibliográficos
Autores principales:	Suzuki, Teruhiko, Kazuki, Yasuhiro, Oshimura, Mitsuo, Hara, Takahiko
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2014
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4193884/ https://www.ncbi.nlm.nih.gov/pubmed/25303219 http://dx.doi.org/10.1371/journal.pone.0110404

Ejemplares similares

Highly Efficient Transfer of Chromosomes to a Broad Range of Target Cells Using Chinese Hamster Ovary Cells Expressing Murine Leukemia Virus-Derived Envelope Proteins
por: Suzuki, Teruhiko, et al.
Publicado: (2016)

Transfer of a Mouse Artificial Chromosome into Spermatogonial Stem Cells Generates Transchromosomic Mice
por: Shinohara, Takashi, et al.
Publicado: (2017)

Development of a Safeguard System Using an Episomal Mammalian Artificial Chromosome for Gene and Cell Therapy
por: Uno, Narumi, et al.
Publicado: (2015)

Development of a multiple-gene-loading method by combining multi-integration system-equipped mouse artificial chromosome vector and CRISPR-Cas9
por: Honma, Kazuhisa, et al.
Publicado: (2018)

A Method for Producing Transgenic Cells Using a Multi-Integrase System on a Human Artificial Chromosome Vector
por: Yamaguchi, Shigeyuki, et al.
Publicado: (2011)

Simultaneous loading of PCR-based multiple fragments on mouse artificial chromosome vectors in DT40 cell for gene delivery
por: Yamazaki, Kyotaro, et al.
Publicado: (2022)

A luciferase complementation assay system using transferable mouse artificial chromosomes to monitor protein–protein interactions mediated by G protein-coupled receptors
por: Uno, Narumi, et al.
Publicado: (2018)

Publisher Correction: Simultaneous loading of PCR-based multiple fragments on mouse artificial chromosome vectors in DT40 cell for gene delivery
por: Yamazaki, Kyotaro, et al.
Publicado: (2023)

A Human Artificial Chromosome Recapitulates the Metabolism of Native Telomeres in Mammalian Cells
por: Wakai, Michihito, et al.
Publicado: (2014)

A pathway from chromosome transfer to engineering resulting in human and mouse artificial chromosomes for a variety of applications to bio-medical challenges
por: Oshimura, Mitsuo, et al.
Publicado: (2015)

A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing
por: Suzuki, Teruhiko, et al.
Publicado: (2021)

Construction of stable mouse artificial chromosome from native mouse chromosome 10 for generation of transchromosomic mice
por: Abe, Satoshi, et al.
Publicado: (2021)

Development of Caco-2 cells expressing four CYPs via a mammalian artificial chromosome
por: Ohta, Yumi, et al.
Publicado: (2020)

Integration-Free iPS Cells Engineered Using Human Artificial Chromosome Vectors
por: Hiratsuka, Masaharu, et al.
Publicado: (2011)

An efficient protein production system via gene amplification on a human artificial chromosome and the chromosome transfer to CHO cells
por: Ohira, Takahito, et al.
Publicado: (2019)

Panel of human cell lines with human/mouse artificial chromosomes
por: Uno, Narumi, et al.
Publicado: (2022)

Mouse embryonic stem cells with a multi-integrase mouse artificial chromosome for transchromosomic mouse generation
por: Yoshimura, Yuki, et al.
Publicado: (2015)

Establishment of a novel hepatocyte model that expresses four cytochrome P450 genes stably via mammalian-derived artificial chromosome for pharmacokinetics and toxicity studies
por: Satoh, Daisuke, et al.
Publicado: (2017)

Use of a Human Artificial Chromosome for Delivering Trophic Factors in a Rodent Model of Amyotrophic Lateral Sclerosis
por: Watanabe, Yasuhiro, et al.
Publicado: (2015)

A kidney injury molecule‐1 (Kim‐1) gene reporter in a mouse artificial chromosome: the responsiveness to cisplatin toxicity in immortalized mouse kidney S3 cells
por: Kokura, Kenji, et al.
Publicado: (2016)

CRISPR/Cas9-induced transgene insertion and telomere-associated truncation of a single human chromosome for chromosome engineering in CHO and A9 cells
por: Uno, Narumi, et al.
Publicado: (2017)

Multiple expression cassette exchange via TP901‐1, R4, and Bxb1 integrase systems on a mouse artificial chromosome
por: Tomimatsu, Kosuke, et al.
Publicado: (2017)

Treatment of CHO cells with Taxol and reversine improves micronucleation and microcell-mediated chromosome transfer efficiency
por: Uno, Narumi, et al.
Publicado: (2023)

Engineering of human induced pluripotent stem cells via human artificial chromosome vectors for cell therapy and disease modeling
por: Kazuki, Yasuhiro, et al.
Publicado: (2020)

Human Artificial Chromosome with a Conditional Centromere for Gene Delivery and Gene Expression
por: Iida, Yuichi, et al.
Publicado: (2010)

Refined human artificial chromosome vectors for gene therapy and animal transgenesis
por: Kazuki, Y, et al.
Publicado: (2011)

Retargeting of microcell fusion towards recipient cell-oriented transfer of human artificial chromosome
por: Hiratsuka, Masaharu, et al.
Publicado: (2015)

Full-length human dystrophin on human artificial chromosome compensates for mouse dystrophin deficiency in a Duchenne muscular dystrophy mouse model
por: Hiramuki, Yosuke, et al.
Publicado: (2023)

Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer
por: Katoh, Motonobu, et al.
Publicado: (2010)

Subchromosomal Mapping of a Putative Transformation Suppressor Gene on Human Chromosome 1
por: Horikawa, Izumi, et al.
Publicado: (1995)

Studies of Tumor Suppressor Genes via Chromosome Engineering
por: Kugoh, Hiroyuki, et al.
Publicado: (2015)

Analysis of in vitro and in vivo metabolism of zidovudine and gemfibrozil in trans‐chromosomic mouse line expressing human UGT2 enzymes
por: Kobayashi, Kaoru, et al.
Publicado: (2022)

Dysregulation of Gene Expression in the Artificial Human Trisomy Cells of Chromosome 8 Associated with Transformed Cell Phenotypes
por: Nawata, Hisakatsu, et al.
Publicado: (2011)

Overexpression screen of chromosome 21 genes reveals modulators of Sonic hedgehog signaling relevant to Down syndrome
por: Moyer, Anna J., et al.
Publicado: (2023)

Bioluminescence Measurement of Time-Dependent Dynamic Changes of CYP-Mediated Cytotoxicity in CYP-Expressing Luminescent HepG2 Cells
por: Iwado, Satoru, et al.
Publicado: (2021)

Hypermetabolism in mice carrying a near complete human chromosome 21
por: Sarver, Dylan C., et al.
Publicado: (2023)

Hypermetabolism in mice carrying a near-complete human chromosome 21
por: Sarver, Dylan C, et al.
Publicado: (2023)

Construction of Mouse A9 Clones Containing a Single Human Chromosome (X/Autosome Translocation) via Micro‐cell Fusion
por: Koi, Minoru, et al.
Publicado: (1989)

Reversible immortalisation enables genetic correction of human muscle progenitors and engineering of next‐generation human artificial chromosomes for Duchenne muscular dystrophy
por: Benedetti, Sara, et al.
Publicado: (2017)

Construction of Mouse A9 Clones Containing a Single Human Chromosome Tagged with Neomycin‐resistance Gene via Microcell Fusion
por: Koi, Minoru, et al.
Publicado: (1989)

Cannot write session to /tmp/vufind_sessions/sess_2let1nmdmbn3k7ao0ai55eecd4