Cargando…

Cytotoxic effects of cobalt and nickel ions on osteocytes in vitro

BACKGROUND: Metal-on-metal prostheses undergo wear and corrosion, releasing soluble ions and wear particles into the surrounding environment. Reports described early failures of the metal-on-metal prostheses, with histologic features similar to a Type IV immune response. Mechanisms by which metal we...

Descripción completa

Detalles Bibliográficos
Autores principales: Kanaji, Arihiko, Orhue, Vbenosawemwinghaye, Caicedo, Marco S, Virdi, Amarjit S, Sumner, Dale R, Hallab, Nadim J, Yoshiaki, Toyama, Sena, Kotaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4194407/
https://www.ncbi.nlm.nih.gov/pubmed/25288055
http://dx.doi.org/10.1186/s13018-014-0091-6
_version_ 1782339109276090368
author Kanaji, Arihiko
Orhue, Vbenosawemwinghaye
Caicedo, Marco S
Virdi, Amarjit S
Sumner, Dale R
Hallab, Nadim J
Yoshiaki, Toyama
Sena, Kotaro
author_facet Kanaji, Arihiko
Orhue, Vbenosawemwinghaye
Caicedo, Marco S
Virdi, Amarjit S
Sumner, Dale R
Hallab, Nadim J
Yoshiaki, Toyama
Sena, Kotaro
author_sort Kanaji, Arihiko
collection PubMed
description BACKGROUND: Metal-on-metal prostheses undergo wear and corrosion, releasing soluble ions and wear particles into the surrounding environment. Reports described early failures of the metal-on-metal prostheses, with histologic features similar to a Type IV immune response. Mechanisms by which metal wear products and metal ion causing this reaction are not completely understood, and the effects of metal ions on osteocytes, which represent more than 95% of all the bone cells, have not been also studied. We hypothesized that soluble metal ions released from the cobalt-chromium-molybdenum (Co-Cr-Mo) prosthesis may have cytotoxic effect on osteocytes. METHODS: MLO-Y4 osteocytes were treated with various metal ion solutions for 24 and 48 h. The effect of ion treatment on cytotoxicity was assessed by WST-1 reagents and cell death ELISA. Morphological changes were analyzed by a phase-contrast microscope or fluorescent microscope using Hoechst 33342 and propidium iodine staining. RESULTS: Cr and Mo ions did not cause cell death under 0.50 mM, highest concentration studied, whereas Co and Ni ions had significant cytotoxic effect on MLO-Y4 cells at concentrations grater than 0.10 mM and at 0.50 mM, respectively, in a dose-dependent manner. According to the ELISA data, osteocytes treated with Co ions were more susceptible to necrotic than apoptotic cell death, while Ni ions caused osteocyte apoptosis. The morphological assays show that cells treated with Co and Ni ions at high concentration were fewer in number and rounded. In addition, fluorescent images showed a marked reduction in live cells and an increase in dead osteocytes treated with Co and Ni ions at high concentration. CONCLUSIONS: Metal ions released from metal-on-metal bearing surfaces have potentially cytotoxic effects on MLO-Y4 osteocytes, in vitro.
format Online
Article
Text
id pubmed-4194407
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-41944072014-10-14 Cytotoxic effects of cobalt and nickel ions on osteocytes in vitro Kanaji, Arihiko Orhue, Vbenosawemwinghaye Caicedo, Marco S Virdi, Amarjit S Sumner, Dale R Hallab, Nadim J Yoshiaki, Toyama Sena, Kotaro J Orthop Surg Res Research Article BACKGROUND: Metal-on-metal prostheses undergo wear and corrosion, releasing soluble ions and wear particles into the surrounding environment. Reports described early failures of the metal-on-metal prostheses, with histologic features similar to a Type IV immune response. Mechanisms by which metal wear products and metal ion causing this reaction are not completely understood, and the effects of metal ions on osteocytes, which represent more than 95% of all the bone cells, have not been also studied. We hypothesized that soluble metal ions released from the cobalt-chromium-molybdenum (Co-Cr-Mo) prosthesis may have cytotoxic effect on osteocytes. METHODS: MLO-Y4 osteocytes were treated with various metal ion solutions for 24 and 48 h. The effect of ion treatment on cytotoxicity was assessed by WST-1 reagents and cell death ELISA. Morphological changes were analyzed by a phase-contrast microscope or fluorescent microscope using Hoechst 33342 and propidium iodine staining. RESULTS: Cr and Mo ions did not cause cell death under 0.50 mM, highest concentration studied, whereas Co and Ni ions had significant cytotoxic effect on MLO-Y4 cells at concentrations grater than 0.10 mM and at 0.50 mM, respectively, in a dose-dependent manner. According to the ELISA data, osteocytes treated with Co ions were more susceptible to necrotic than apoptotic cell death, while Ni ions caused osteocyte apoptosis. The morphological assays show that cells treated with Co and Ni ions at high concentration were fewer in number and rounded. In addition, fluorescent images showed a marked reduction in live cells and an increase in dead osteocytes treated with Co and Ni ions at high concentration. CONCLUSIONS: Metal ions released from metal-on-metal bearing surfaces have potentially cytotoxic effects on MLO-Y4 osteocytes, in vitro. BioMed Central 2014-10-08 /pmc/articles/PMC4194407/ /pubmed/25288055 http://dx.doi.org/10.1186/s13018-014-0091-6 Text en © Kanaji et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Kanaji, Arihiko
Orhue, Vbenosawemwinghaye
Caicedo, Marco S
Virdi, Amarjit S
Sumner, Dale R
Hallab, Nadim J
Yoshiaki, Toyama
Sena, Kotaro
Cytotoxic effects of cobalt and nickel ions on osteocytes in vitro
title Cytotoxic effects of cobalt and nickel ions on osteocytes in vitro
title_full Cytotoxic effects of cobalt and nickel ions on osteocytes in vitro
title_fullStr Cytotoxic effects of cobalt and nickel ions on osteocytes in vitro
title_full_unstemmed Cytotoxic effects of cobalt and nickel ions on osteocytes in vitro
title_short Cytotoxic effects of cobalt and nickel ions on osteocytes in vitro
title_sort cytotoxic effects of cobalt and nickel ions on osteocytes in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4194407/
https://www.ncbi.nlm.nih.gov/pubmed/25288055
http://dx.doi.org/10.1186/s13018-014-0091-6
work_keys_str_mv AT kanajiarihiko cytotoxiceffectsofcobaltandnickelionsonosteocytesinvitro
AT orhuevbenosawemwinghaye cytotoxiceffectsofcobaltandnickelionsonosteocytesinvitro
AT caicedomarcos cytotoxiceffectsofcobaltandnickelionsonosteocytesinvitro
AT virdiamarjits cytotoxiceffectsofcobaltandnickelionsonosteocytesinvitro
AT sumnerdaler cytotoxiceffectsofcobaltandnickelionsonosteocytesinvitro
AT hallabnadimj cytotoxiceffectsofcobaltandnickelionsonosteocytesinvitro
AT yoshiakitoyama cytotoxiceffectsofcobaltandnickelionsonosteocytesinvitro
AT senakotaro cytotoxiceffectsofcobaltandnickelionsonosteocytesinvitro