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Discriminative stimulus effects of the novel imidazoline I(2) receptor ligand CR4056 in rats

This study examined whether a novel imidazoline I(2) receptor ligand CR4056 could serve as a discriminative stimulus and whether it shares similar discriminative stimulus effects with other reported I(2) receptor ligands. Eight male Sprague-Dawley rats were trained to discriminate 10.0 mg/kg CR4056...

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Autores principales: Qiu, Yanyan, He, Xiao-Hua, Zhang, Yanan, Li, Jun-Xu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4194429/
https://www.ncbi.nlm.nih.gov/pubmed/25308382
http://dx.doi.org/10.1038/srep06605
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author Qiu, Yanyan
He, Xiao-Hua
Zhang, Yanan
Li, Jun-Xu
author_facet Qiu, Yanyan
He, Xiao-Hua
Zhang, Yanan
Li, Jun-Xu
author_sort Qiu, Yanyan
collection PubMed
description This study examined whether a novel imidazoline I(2) receptor ligand CR4056 could serve as a discriminative stimulus and whether it shares similar discriminative stimulus effects with other reported I(2) receptor ligands. Eight male Sprague-Dawley rats were trained to discriminate 10.0 mg/kg CR4056 (i.p.) from vehicle in a two-lever food-reinforced drug discrimination procedure. Once rats acquired the discrimination, substitution and combination studies were conducted to elucidate the underlying receptor mechanisms. All rats acquired CR4056 discrimination after an average of 26 training sessions. Several I(2) receptor ligands (phenyzoline, tracizoline, RS45041, and idazoxan, 3.2–75 mg/kg, i.p.) all occasioned > 80% CR4056-associated lever responding. Other drugs that occasioned partial or no CR4056-associated lever responding included methamphetamine, ketamine, the endogenous imidazoline ligand agmatine, the monoamine oxidase (MAO) inhibitor harmane, the α(2)-adrenoceptor agonist clonidine, the μ-opioid receptor agonists morphine and methadone, and the selective I(2) receptor ligands BU224 and 2-BFI. The α(1) adrenoceptor antagonist WB4101, α(2) adrenoceptor antagonist yohimbine and μ-opioid receptor antagonist naltrexone failed to alter the stimulus effects of CR4056. Together, these results show that CR4056 can serve as a discriminative stimulus in rats, which demonstrates high pharmacological specificity and appears to be mediated by imidazoline I(2) receptors.
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spelling pubmed-41944292014-10-21 Discriminative stimulus effects of the novel imidazoline I(2) receptor ligand CR4056 in rats Qiu, Yanyan He, Xiao-Hua Zhang, Yanan Li, Jun-Xu Sci Rep Article This study examined whether a novel imidazoline I(2) receptor ligand CR4056 could serve as a discriminative stimulus and whether it shares similar discriminative stimulus effects with other reported I(2) receptor ligands. Eight male Sprague-Dawley rats were trained to discriminate 10.0 mg/kg CR4056 (i.p.) from vehicle in a two-lever food-reinforced drug discrimination procedure. Once rats acquired the discrimination, substitution and combination studies were conducted to elucidate the underlying receptor mechanisms. All rats acquired CR4056 discrimination after an average of 26 training sessions. Several I(2) receptor ligands (phenyzoline, tracizoline, RS45041, and idazoxan, 3.2–75 mg/kg, i.p.) all occasioned > 80% CR4056-associated lever responding. Other drugs that occasioned partial or no CR4056-associated lever responding included methamphetamine, ketamine, the endogenous imidazoline ligand agmatine, the monoamine oxidase (MAO) inhibitor harmane, the α(2)-adrenoceptor agonist clonidine, the μ-opioid receptor agonists morphine and methadone, and the selective I(2) receptor ligands BU224 and 2-BFI. The α(1) adrenoceptor antagonist WB4101, α(2) adrenoceptor antagonist yohimbine and μ-opioid receptor antagonist naltrexone failed to alter the stimulus effects of CR4056. Together, these results show that CR4056 can serve as a discriminative stimulus in rats, which demonstrates high pharmacological specificity and appears to be mediated by imidazoline I(2) receptors. Nature Publishing Group 2014-10-13 /pmc/articles/PMC4194429/ /pubmed/25308382 http://dx.doi.org/10.1038/srep06605 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-sa/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/
spellingShingle Article
Qiu, Yanyan
He, Xiao-Hua
Zhang, Yanan
Li, Jun-Xu
Discriminative stimulus effects of the novel imidazoline I(2) receptor ligand CR4056 in rats
title Discriminative stimulus effects of the novel imidazoline I(2) receptor ligand CR4056 in rats
title_full Discriminative stimulus effects of the novel imidazoline I(2) receptor ligand CR4056 in rats
title_fullStr Discriminative stimulus effects of the novel imidazoline I(2) receptor ligand CR4056 in rats
title_full_unstemmed Discriminative stimulus effects of the novel imidazoline I(2) receptor ligand CR4056 in rats
title_short Discriminative stimulus effects of the novel imidazoline I(2) receptor ligand CR4056 in rats
title_sort discriminative stimulus effects of the novel imidazoline i(2) receptor ligand cr4056 in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4194429/
https://www.ncbi.nlm.nih.gov/pubmed/25308382
http://dx.doi.org/10.1038/srep06605
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