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Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins
Understanding information flow through neuronal circuits requires knowledge of their synaptic organization. In this study, we utilized fluorescent pre- and postsynaptic markers to map synaptic organization in the Drosophila antennal lobe, the first olfactory processing center. Olfactory receptor neu...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4194450/ https://www.ncbi.nlm.nih.gov/pubmed/25310239 http://dx.doi.org/10.7554/eLife.03726 |
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author | Mosca, Timothy J Luo, Liqun |
author_facet | Mosca, Timothy J Luo, Liqun |
author_sort | Mosca, Timothy J |
collection | PubMed |
description | Understanding information flow through neuronal circuits requires knowledge of their synaptic organization. In this study, we utilized fluorescent pre- and postsynaptic markers to map synaptic organization in the Drosophila antennal lobe, the first olfactory processing center. Olfactory receptor neurons (ORNs) produce a constant synaptic density across different glomeruli. Each ORN within a class contributes nearly identical active zone number. Active zones from ORNs, projection neurons (PNs), and local interneurons have distinct subglomerular and subcellular distributions. The correct number of ORN active zones and PN acetylcholine receptor clusters requires the Teneurins, conserved transmembrane proteins involved in neuromuscular synapse organization and synaptic partner matching. Ten-a acts in ORNs to organize presynaptic active zones via the spectrin cytoskeleton. Ten-m acts in PNs autonomously to regulate acetylcholine receptor cluster number and transsynaptically to regulate ORN active zone number. These studies advanced our ability to assess synaptic architecture in complex CNS circuits and their underlying molecular mechanisms. DOI: http://dx.doi.org/10.7554/eLife.03726.001 |
format | Online Article Text |
id | pubmed-4194450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-41944502014-10-17 Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins Mosca, Timothy J Luo, Liqun eLife Neuroscience Understanding information flow through neuronal circuits requires knowledge of their synaptic organization. In this study, we utilized fluorescent pre- and postsynaptic markers to map synaptic organization in the Drosophila antennal lobe, the first olfactory processing center. Olfactory receptor neurons (ORNs) produce a constant synaptic density across different glomeruli. Each ORN within a class contributes nearly identical active zone number. Active zones from ORNs, projection neurons (PNs), and local interneurons have distinct subglomerular and subcellular distributions. The correct number of ORN active zones and PN acetylcholine receptor clusters requires the Teneurins, conserved transmembrane proteins involved in neuromuscular synapse organization and synaptic partner matching. Ten-a acts in ORNs to organize presynaptic active zones via the spectrin cytoskeleton. Ten-m acts in PNs autonomously to regulate acetylcholine receptor cluster number and transsynaptically to regulate ORN active zone number. These studies advanced our ability to assess synaptic architecture in complex CNS circuits and their underlying molecular mechanisms. DOI: http://dx.doi.org/10.7554/eLife.03726.001 eLife Sciences Publications, Ltd 2014-10-13 /pmc/articles/PMC4194450/ /pubmed/25310239 http://dx.doi.org/10.7554/eLife.03726 Text en Copyright © 2014, Mosca and Luo http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Mosca, Timothy J Luo, Liqun Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins |
title | Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins |
title_full | Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins |
title_fullStr | Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins |
title_full_unstemmed | Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins |
title_short | Synaptic organization of the Drosophila antennal lobe and its regulation by the Teneurins |
title_sort | synaptic organization of the drosophila antennal lobe and its regulation by the teneurins |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4194450/ https://www.ncbi.nlm.nih.gov/pubmed/25310239 http://dx.doi.org/10.7554/eLife.03726 |
work_keys_str_mv | AT moscatimothyj synapticorganizationofthedrosophilaantennallobeanditsregulationbytheteneurins AT luoliqun synapticorganizationofthedrosophilaantennallobeanditsregulationbytheteneurins |