Engineering Bacterial Transcription Regulation To Create a Synthetic in Vitro Two-Hybrid System for Protein Interaction Assays

[Image: see text] Transcriptional activation of σ(54)-RNA polymerase holoenzyme (σ(54)-RNAP) in bacteria is dependent on a cis-acting DNA element (bacterial enhancer), which recruits the bacterial enhancer-binding protein to contact the holoenzyme via DNA looping. Using a constructive synthetic biol...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Ying, Asahara, Haruichi, Schneider, Nils, Dranchak, Patricia, Inglese, James, Chong, Shaorong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4195380/
https://www.ncbi.nlm.nih.gov/pubmed/25188838
http://dx.doi.org/10.1021/ja502512g

Ejemplares similares

Ejemplares similares