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Evolutionary History of Trihelix Family and Their Functional Diversification
In this study, we carried out an evolutionary, transcriptional, and functional analyses of the trihelix transcription factor family. A total of 319 trihelix members, identified from 11 land plant species, were classified into five clades. The results of phylogeny indicate the binding domains of GT1...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4195496/ https://www.ncbi.nlm.nih.gov/pubmed/24864043 http://dx.doi.org/10.1093/dnares/dsu016 |
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author | Qin, Yao Ma, Xin Yu, Guanghui Wang, Qi Wang, Liang Kong, Lingrang Kim, Wook Wang, Hong Wei |
author_facet | Qin, Yao Ma, Xin Yu, Guanghui Wang, Qi Wang, Liang Kong, Lingrang Kim, Wook Wang, Hong Wei |
author_sort | Qin, Yao |
collection | PubMed |
description | In this study, we carried out an evolutionary, transcriptional, and functional analyses of the trihelix transcription factor family. A total of 319 trihelix members, identified from 11 land plant species, were classified into five clades. The results of phylogeny indicate the binding domains of GT1 and GT2 diverged early in the existence of land plants. Genomic localization revealed that the trihelix family members were highly conserved among cereal species, even though some homeologs generated during the tetraploidy of maize were lost. Three-dimensional structural analyses and an examination of subcellular localization of this family supported the involvement of all five clades in transcriptional regulation. Furthermore, the family members from all clades in sorghum and rice showed a broad and dynamic expression pattern in response to abiotic stresses, indicating regulatory subfunctionalization of their original functions. This finding is further supported by the phenotypes of enhanced tolerance to cold, salt, and drought in transgenic plants overexpressing Sb06g023980 and Sb06g024110. In contrast, few Arobidopsis genes showed inducible expression under abiotic stress conditions, which may indicate a functional shift. Finally, our co-expression analysis points to the involvement of this family in various metabolic processes, implying their further functional divergence. |
format | Online Article Text |
id | pubmed-4195496 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-41954962014-10-21 Evolutionary History of Trihelix Family and Their Functional Diversification Qin, Yao Ma, Xin Yu, Guanghui Wang, Qi Wang, Liang Kong, Lingrang Kim, Wook Wang, Hong Wei DNA Res Full Papers In this study, we carried out an evolutionary, transcriptional, and functional analyses of the trihelix transcription factor family. A total of 319 trihelix members, identified from 11 land plant species, were classified into five clades. The results of phylogeny indicate the binding domains of GT1 and GT2 diverged early in the existence of land plants. Genomic localization revealed that the trihelix family members were highly conserved among cereal species, even though some homeologs generated during the tetraploidy of maize were lost. Three-dimensional structural analyses and an examination of subcellular localization of this family supported the involvement of all five clades in transcriptional regulation. Furthermore, the family members from all clades in sorghum and rice showed a broad and dynamic expression pattern in response to abiotic stresses, indicating regulatory subfunctionalization of their original functions. This finding is further supported by the phenotypes of enhanced tolerance to cold, salt, and drought in transgenic plants overexpressing Sb06g023980 and Sb06g024110. In contrast, few Arobidopsis genes showed inducible expression under abiotic stress conditions, which may indicate a functional shift. Finally, our co-expression analysis points to the involvement of this family in various metabolic processes, implying their further functional divergence. Oxford University Press 2014-10 2014-05-25 /pmc/articles/PMC4195496/ /pubmed/24864043 http://dx.doi.org/10.1093/dnares/dsu016 Text en © The Author 2014. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Qin, Yao Ma, Xin Yu, Guanghui Wang, Qi Wang, Liang Kong, Lingrang Kim, Wook Wang, Hong Wei Evolutionary History of Trihelix Family and Their Functional Diversification |
title | Evolutionary History of Trihelix Family and Their Functional Diversification |
title_full | Evolutionary History of Trihelix Family and Their Functional Diversification |
title_fullStr | Evolutionary History of Trihelix Family and Their Functional Diversification |
title_full_unstemmed | Evolutionary History of Trihelix Family and Their Functional Diversification |
title_short | Evolutionary History of Trihelix Family and Their Functional Diversification |
title_sort | evolutionary history of trihelix family and their functional diversification |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4195496/ https://www.ncbi.nlm.nih.gov/pubmed/24864043 http://dx.doi.org/10.1093/dnares/dsu016 |
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