Cargando…

Different Expression Patterns of Genes from the Exo-Xis Region of Bacteriophage λ and Shiga Toxin-Converting Bacteriophage Ф24(B) following Infection or Prophage Induction in Escherichia coli

Lambdoid bacteriophages serve as useful models in microbiological and molecular studies on basic biological process. Moreover, this family of viruses plays an important role in pathogenesis of enterohemorrhagic Escherichia coli (EHEC) strains, as they are carriers of genes coding for Shiga toxins. E...

Descripción completa

Detalles Bibliográficos
Autores principales: Bloch, Sylwia, Nejman-Faleńczyk, Bożena, Dydecka, Aleksandra, Łoś, Joanna M., Felczykowska, Agnieszka, Węgrzyn, Alicja, Węgrzyn, Grzegorz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4195576/
https://www.ncbi.nlm.nih.gov/pubmed/25310402
http://dx.doi.org/10.1371/journal.pone.0108233
_version_ 1782339325560619008
author Bloch, Sylwia
Nejman-Faleńczyk, Bożena
Dydecka, Aleksandra
Łoś, Joanna M.
Felczykowska, Agnieszka
Węgrzyn, Alicja
Węgrzyn, Grzegorz
author_facet Bloch, Sylwia
Nejman-Faleńczyk, Bożena
Dydecka, Aleksandra
Łoś, Joanna M.
Felczykowska, Agnieszka
Węgrzyn, Alicja
Węgrzyn, Grzegorz
author_sort Bloch, Sylwia
collection PubMed
description Lambdoid bacteriophages serve as useful models in microbiological and molecular studies on basic biological process. Moreover, this family of viruses plays an important role in pathogenesis of enterohemorrhagic Escherichia coli (EHEC) strains, as they are carriers of genes coding for Shiga toxins. Efficient expression of these genes requires lambdoid prophage induction and multiplication of the phage genome. Therefore, understanding the mechanisms regulating these processes appears essential for both basic knowledge and potential anti-EHEC applications. The exo-xis region, present in genomes of lambdoid bacteriophages, contains highly conserved genes of largely unknown functions. Recent report indicated that the Ea8.5 protein, encoded in this region, contains a newly discovered fused homeodomain/zinc-finger fold, suggesting its plausible regulatory role. Moreover, subsequent studies demonstrated that overexpression of the exo-xis region from a multicopy plasmid resulted in impaired lysogenization of E. coli and more effective induction of λ and Ф24(B) prophages. In this report, we demonstrate that after prophage induction, the increase in phage DNA content in the host cells is more efficient in E. coli bearing additional copies of the exo-xis region, while survival rate of such bacteria is lower, which corroborated previous observations. Importantly, by using quantitative real-time reverse transcription PCR, we have determined patterns of expressions of particular genes from this region. Unexpectedly, in both phages λ and Ф24(B), these patterns were significantly different not only between conditions of the host cells infection by bacteriophages and prophage induction, but also between induction of prophages with various agents (mitomycin C and hydrogen peroxide). This may shed a new light on our understanding of regulation of lambdoid phage development, depending on the mode of lytic cycle initiation.
format Online
Article
Text
id pubmed-4195576
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-41955762014-10-15 Different Expression Patterns of Genes from the Exo-Xis Region of Bacteriophage λ and Shiga Toxin-Converting Bacteriophage Ф24(B) following Infection or Prophage Induction in Escherichia coli Bloch, Sylwia Nejman-Faleńczyk, Bożena Dydecka, Aleksandra Łoś, Joanna M. Felczykowska, Agnieszka Węgrzyn, Alicja Węgrzyn, Grzegorz PLoS One Research Article Lambdoid bacteriophages serve as useful models in microbiological and molecular studies on basic biological process. Moreover, this family of viruses plays an important role in pathogenesis of enterohemorrhagic Escherichia coli (EHEC) strains, as they are carriers of genes coding for Shiga toxins. Efficient expression of these genes requires lambdoid prophage induction and multiplication of the phage genome. Therefore, understanding the mechanisms regulating these processes appears essential for both basic knowledge and potential anti-EHEC applications. The exo-xis region, present in genomes of lambdoid bacteriophages, contains highly conserved genes of largely unknown functions. Recent report indicated that the Ea8.5 protein, encoded in this region, contains a newly discovered fused homeodomain/zinc-finger fold, suggesting its plausible regulatory role. Moreover, subsequent studies demonstrated that overexpression of the exo-xis region from a multicopy plasmid resulted in impaired lysogenization of E. coli and more effective induction of λ and Ф24(B) prophages. In this report, we demonstrate that after prophage induction, the increase in phage DNA content in the host cells is more efficient in E. coli bearing additional copies of the exo-xis region, while survival rate of such bacteria is lower, which corroborated previous observations. Importantly, by using quantitative real-time reverse transcription PCR, we have determined patterns of expressions of particular genes from this region. Unexpectedly, in both phages λ and Ф24(B), these patterns were significantly different not only between conditions of the host cells infection by bacteriophages and prophage induction, but also between induction of prophages with various agents (mitomycin C and hydrogen peroxide). This may shed a new light on our understanding of regulation of lambdoid phage development, depending on the mode of lytic cycle initiation. Public Library of Science 2014-10-13 /pmc/articles/PMC4195576/ /pubmed/25310402 http://dx.doi.org/10.1371/journal.pone.0108233 Text en © 2014 Bloch et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bloch, Sylwia
Nejman-Faleńczyk, Bożena
Dydecka, Aleksandra
Łoś, Joanna M.
Felczykowska, Agnieszka
Węgrzyn, Alicja
Węgrzyn, Grzegorz
Different Expression Patterns of Genes from the Exo-Xis Region of Bacteriophage λ and Shiga Toxin-Converting Bacteriophage Ф24(B) following Infection or Prophage Induction in Escherichia coli
title Different Expression Patterns of Genes from the Exo-Xis Region of Bacteriophage λ and Shiga Toxin-Converting Bacteriophage Ф24(B) following Infection or Prophage Induction in Escherichia coli
title_full Different Expression Patterns of Genes from the Exo-Xis Region of Bacteriophage λ and Shiga Toxin-Converting Bacteriophage Ф24(B) following Infection or Prophage Induction in Escherichia coli
title_fullStr Different Expression Patterns of Genes from the Exo-Xis Region of Bacteriophage λ and Shiga Toxin-Converting Bacteriophage Ф24(B) following Infection or Prophage Induction in Escherichia coli
title_full_unstemmed Different Expression Patterns of Genes from the Exo-Xis Region of Bacteriophage λ and Shiga Toxin-Converting Bacteriophage Ф24(B) following Infection or Prophage Induction in Escherichia coli
title_short Different Expression Patterns of Genes from the Exo-Xis Region of Bacteriophage λ and Shiga Toxin-Converting Bacteriophage Ф24(B) following Infection or Prophage Induction in Escherichia coli
title_sort different expression patterns of genes from the exo-xis region of bacteriophage λ and shiga toxin-converting bacteriophage ф24(b) following infection or prophage induction in escherichia coli
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4195576/
https://www.ncbi.nlm.nih.gov/pubmed/25310402
http://dx.doi.org/10.1371/journal.pone.0108233
work_keys_str_mv AT blochsylwia differentexpressionpatternsofgenesfromtheexoxisregionofbacteriophagelandshigatoxinconvertingbacteriophagef24bfollowinginfectionorprophageinductioninescherichiacoli
AT nejmanfalenczykbozena differentexpressionpatternsofgenesfromtheexoxisregionofbacteriophagelandshigatoxinconvertingbacteriophagef24bfollowinginfectionorprophageinductioninescherichiacoli
AT dydeckaaleksandra differentexpressionpatternsofgenesfromtheexoxisregionofbacteriophagelandshigatoxinconvertingbacteriophagef24bfollowinginfectionorprophageinductioninescherichiacoli
AT łosjoannam differentexpressionpatternsofgenesfromtheexoxisregionofbacteriophagelandshigatoxinconvertingbacteriophagef24bfollowinginfectionorprophageinductioninescherichiacoli
AT felczykowskaagnieszka differentexpressionpatternsofgenesfromtheexoxisregionofbacteriophagelandshigatoxinconvertingbacteriophagef24bfollowinginfectionorprophageinductioninescherichiacoli
AT wegrzynalicja differentexpressionpatternsofgenesfromtheexoxisregionofbacteriophagelandshigatoxinconvertingbacteriophagef24bfollowinginfectionorprophageinductioninescherichiacoli
AT wegrzyngrzegorz differentexpressionpatternsofgenesfromtheexoxisregionofbacteriophagelandshigatoxinconvertingbacteriophagef24bfollowinginfectionorprophageinductioninescherichiacoli