Cargando…

RNA-Seq Analysis of the Effect of Kanamycin and the ABC Transporter AtWBC19 on Arabidopsis thaliana Seedlings Reveals Changes in Metal Content

Plants are exposed to antibiotics produced by soil microorganisms, but little is known about their responses at the transcriptional level. Likewise, few endogenous mechanisms of antibiotic resistance have been reported. The Arabidopsis thaliana ATP Binding Cassette (ABC) transporter AtWBC19 (ABCG19)...

Descripción completa

Detalles Bibliográficos
Autores principales: Mentewab, Ayalew, Matheson, Kinnari, Adebiyi, Morayo, Robinson, Shanice, Elston, Brianna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4195610/
https://www.ncbi.nlm.nih.gov/pubmed/25310285
http://dx.doi.org/10.1371/journal.pone.0109310
_version_ 1782339331482976256
author Mentewab, Ayalew
Matheson, Kinnari
Adebiyi, Morayo
Robinson, Shanice
Elston, Brianna
author_facet Mentewab, Ayalew
Matheson, Kinnari
Adebiyi, Morayo
Robinson, Shanice
Elston, Brianna
author_sort Mentewab, Ayalew
collection PubMed
description Plants are exposed to antibiotics produced by soil microorganisms, but little is known about their responses at the transcriptional level. Likewise, few endogenous mechanisms of antibiotic resistance have been reported. The Arabidopsis thaliana ATP Binding Cassette (ABC) transporter AtWBC19 (ABCG19) is known to confer kanamycin resistance, but the exact mechanism of resistance is not well understood. Here we examined the transcriptomes of control seedlings and wbc19 mutant seedlings using RNA-seq analysis. Exposure to kanamycin indicated changes in the organization of the photosynthetic apparatus, metabolic fluxes and metal uptake. Elemental analysis showed a 60% and 80% reduction of iron uptake in control and wbc19 mutant seedlings respectively, upon exposure to kanamycin. The drop in iron content was accompanied by the upregulation of the gene encoding for FERRIC REDUCTION OXIDASE 6 (FRO6) in mutant seedlings but not by the differential expression of other transport genes known to be induced by iron deficiency. In addition, wbc19 mutants displayed a distinct expression profile in the absence of kanamycin. Most notably the expression of several zinc ion binding proteins, including ZINC TRANSPORTER 1 PRECURSOR (ZIP1) was increased, suggesting abnormal zinc uptake. Elemental analysis confirmed a 50% decrease of zinc content in wbc19 mutants. Thus, the antibiotic resistance gene WBC19 appears to also have a role in zinc uptake.
format Online
Article
Text
id pubmed-4195610
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-41956102014-10-15 RNA-Seq Analysis of the Effect of Kanamycin and the ABC Transporter AtWBC19 on Arabidopsis thaliana Seedlings Reveals Changes in Metal Content Mentewab, Ayalew Matheson, Kinnari Adebiyi, Morayo Robinson, Shanice Elston, Brianna PLoS One Research Article Plants are exposed to antibiotics produced by soil microorganisms, but little is known about their responses at the transcriptional level. Likewise, few endogenous mechanisms of antibiotic resistance have been reported. The Arabidopsis thaliana ATP Binding Cassette (ABC) transporter AtWBC19 (ABCG19) is known to confer kanamycin resistance, but the exact mechanism of resistance is not well understood. Here we examined the transcriptomes of control seedlings and wbc19 mutant seedlings using RNA-seq analysis. Exposure to kanamycin indicated changes in the organization of the photosynthetic apparatus, metabolic fluxes and metal uptake. Elemental analysis showed a 60% and 80% reduction of iron uptake in control and wbc19 mutant seedlings respectively, upon exposure to kanamycin. The drop in iron content was accompanied by the upregulation of the gene encoding for FERRIC REDUCTION OXIDASE 6 (FRO6) in mutant seedlings but not by the differential expression of other transport genes known to be induced by iron deficiency. In addition, wbc19 mutants displayed a distinct expression profile in the absence of kanamycin. Most notably the expression of several zinc ion binding proteins, including ZINC TRANSPORTER 1 PRECURSOR (ZIP1) was increased, suggesting abnormal zinc uptake. Elemental analysis confirmed a 50% decrease of zinc content in wbc19 mutants. Thus, the antibiotic resistance gene WBC19 appears to also have a role in zinc uptake. Public Library of Science 2014-10-13 /pmc/articles/PMC4195610/ /pubmed/25310285 http://dx.doi.org/10.1371/journal.pone.0109310 Text en © 2014 Mentewab et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mentewab, Ayalew
Matheson, Kinnari
Adebiyi, Morayo
Robinson, Shanice
Elston, Brianna
RNA-Seq Analysis of the Effect of Kanamycin and the ABC Transporter AtWBC19 on Arabidopsis thaliana Seedlings Reveals Changes in Metal Content
title RNA-Seq Analysis of the Effect of Kanamycin and the ABC Transporter AtWBC19 on Arabidopsis thaliana Seedlings Reveals Changes in Metal Content
title_full RNA-Seq Analysis of the Effect of Kanamycin and the ABC Transporter AtWBC19 on Arabidopsis thaliana Seedlings Reveals Changes in Metal Content
title_fullStr RNA-Seq Analysis of the Effect of Kanamycin and the ABC Transporter AtWBC19 on Arabidopsis thaliana Seedlings Reveals Changes in Metal Content
title_full_unstemmed RNA-Seq Analysis of the Effect of Kanamycin and the ABC Transporter AtWBC19 on Arabidopsis thaliana Seedlings Reveals Changes in Metal Content
title_short RNA-Seq Analysis of the Effect of Kanamycin and the ABC Transporter AtWBC19 on Arabidopsis thaliana Seedlings Reveals Changes in Metal Content
title_sort rna-seq analysis of the effect of kanamycin and the abc transporter atwbc19 on arabidopsis thaliana seedlings reveals changes in metal content
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4195610/
https://www.ncbi.nlm.nih.gov/pubmed/25310285
http://dx.doi.org/10.1371/journal.pone.0109310
work_keys_str_mv AT mentewabayalew rnaseqanalysisoftheeffectofkanamycinandtheabctransporteratwbc19onarabidopsisthalianaseedlingsrevealschangesinmetalcontent
AT mathesonkinnari rnaseqanalysisoftheeffectofkanamycinandtheabctransporteratwbc19onarabidopsisthalianaseedlingsrevealschangesinmetalcontent
AT adebiyimorayo rnaseqanalysisoftheeffectofkanamycinandtheabctransporteratwbc19onarabidopsisthalianaseedlingsrevealschangesinmetalcontent
AT robinsonshanice rnaseqanalysisoftheeffectofkanamycinandtheabctransporteratwbc19onarabidopsisthalianaseedlingsrevealschangesinmetalcontent
AT elstonbrianna rnaseqanalysisoftheeffectofkanamycinandtheabctransporteratwbc19onarabidopsisthalianaseedlingsrevealschangesinmetalcontent