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Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells
BACKGROUND: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients. High glucose could increase endothelial permeability and cancer-associated signaling. These suggest high glucose may affect H. pylori or its infected status. We used two strains to investigate whether H. p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196111/ https://www.ncbi.nlm.nih.gov/pubmed/25296847 http://dx.doi.org/10.1186/s12929-014-0096-9 |
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author | Sheu, Shew-Meei Cheng, Hsin Kao, Cheng-Ye Yang, Yao-Jong Wu, Jiunn-Jong Sheu, Bor-Shyang |
author_facet | Sheu, Shew-Meei Cheng, Hsin Kao, Cheng-Ye Yang, Yao-Jong Wu, Jiunn-Jong Sheu, Bor-Shyang |
author_sort | Sheu, Shew-Meei |
collection | PubMed |
description | BACKGROUND: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients. High glucose could increase endothelial permeability and cancer-associated signaling. These suggest high glucose may affect H. pylori or its infected status. We used two strains to investigate whether H. pylori growth, viability, adhesion and CagA-phosphorylation level in the infected-AGS cells were influenced by glucose concentration (100, 150, and 200 mg/dL). RESULTS: The growth curves of both strains in 200 mg/dL of glucose were maintained at the highest optimal density after 48 h and the best viability of both strains were retained in the same glucose condition at 72 h. Furthermore, adhesion enhancement of H. pylori was significantly higher in 200 mg/dL of glucose as compared to that in 100 and 150 mg/dL (p < 0.05). CagA protein also increased in higher glucose condition. The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent. CONCLUSION: Higher glucose could maintain H. pylori growth and viability after 48 h. H. pylori adhesion and CagA increased to further facilitate the enhancement of cell-associated CagA and phosphorylated CagA in higher glucose conditions. |
format | Online Article Text |
id | pubmed-4196111 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41961112014-10-15 Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells Sheu, Shew-Meei Cheng, Hsin Kao, Cheng-Ye Yang, Yao-Jong Wu, Jiunn-Jong Sheu, Bor-Shyang J Biomed Sci Research BACKGROUND: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients. High glucose could increase endothelial permeability and cancer-associated signaling. These suggest high glucose may affect H. pylori or its infected status. We used two strains to investigate whether H. pylori growth, viability, adhesion and CagA-phosphorylation level in the infected-AGS cells were influenced by glucose concentration (100, 150, and 200 mg/dL). RESULTS: The growth curves of both strains in 200 mg/dL of glucose were maintained at the highest optimal density after 48 h and the best viability of both strains were retained in the same glucose condition at 72 h. Furthermore, adhesion enhancement of H. pylori was significantly higher in 200 mg/dL of glucose as compared to that in 100 and 150 mg/dL (p < 0.05). CagA protein also increased in higher glucose condition. The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent. CONCLUSION: Higher glucose could maintain H. pylori growth and viability after 48 h. H. pylori adhesion and CagA increased to further facilitate the enhancement of cell-associated CagA and phosphorylated CagA in higher glucose conditions. BioMed Central 2014-10-09 /pmc/articles/PMC4196111/ /pubmed/25296847 http://dx.doi.org/10.1186/s12929-014-0096-9 Text en © Sheu et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Sheu, Shew-Meei Cheng, Hsin Kao, Cheng-Ye Yang, Yao-Jong Wu, Jiunn-Jong Sheu, Bor-Shyang Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells |
title | Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells |
title_full | Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells |
title_fullStr | Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells |
title_full_unstemmed | Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells |
title_short | Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells |
title_sort | higher glucose level can enhance the h. pylori adhesion and virulence related with type iv secretion system in ags cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196111/ https://www.ncbi.nlm.nih.gov/pubmed/25296847 http://dx.doi.org/10.1186/s12929-014-0096-9 |
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