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Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells

BACKGROUND: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients. High glucose could increase endothelial permeability and cancer-associated signaling. These suggest high glucose may affect H. pylori or its infected status. We used two strains to investigate whether H. p...

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Autores principales: Sheu, Shew-Meei, Cheng, Hsin, Kao, Cheng-Ye, Yang, Yao-Jong, Wu, Jiunn-Jong, Sheu, Bor-Shyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196111/
https://www.ncbi.nlm.nih.gov/pubmed/25296847
http://dx.doi.org/10.1186/s12929-014-0096-9
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author Sheu, Shew-Meei
Cheng, Hsin
Kao, Cheng-Ye
Yang, Yao-Jong
Wu, Jiunn-Jong
Sheu, Bor-Shyang
author_facet Sheu, Shew-Meei
Cheng, Hsin
Kao, Cheng-Ye
Yang, Yao-Jong
Wu, Jiunn-Jong
Sheu, Bor-Shyang
author_sort Sheu, Shew-Meei
collection PubMed
description BACKGROUND: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients. High glucose could increase endothelial permeability and cancer-associated signaling. These suggest high glucose may affect H. pylori or its infected status. We used two strains to investigate whether H. pylori growth, viability, adhesion and CagA-phosphorylation level in the infected-AGS cells were influenced by glucose concentration (100, 150, and 200 mg/dL). RESULTS: The growth curves of both strains in 200 mg/dL of glucose were maintained at the highest optimal density after 48 h and the best viability of both strains were retained in the same glucose condition at 72 h. Furthermore, adhesion enhancement of H. pylori was significantly higher in 200 mg/dL of glucose as compared to that in 100 and 150 mg/dL (p < 0.05). CagA protein also increased in higher glucose condition. The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent. CONCLUSION: Higher glucose could maintain H. pylori growth and viability after 48 h. H. pylori adhesion and CagA increased to further facilitate the enhancement of cell-associated CagA and phosphorylated CagA in higher glucose conditions.
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spelling pubmed-41961112014-10-15 Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells Sheu, Shew-Meei Cheng, Hsin Kao, Cheng-Ye Yang, Yao-Jong Wu, Jiunn-Jong Sheu, Bor-Shyang J Biomed Sci Research BACKGROUND: Hyperglycemia increases the risk of gastric cancer in H. pylori-infected patients. High glucose could increase endothelial permeability and cancer-associated signaling. These suggest high glucose may affect H. pylori or its infected status. We used two strains to investigate whether H. pylori growth, viability, adhesion and CagA-phosphorylation level in the infected-AGS cells were influenced by glucose concentration (100, 150, and 200 mg/dL). RESULTS: The growth curves of both strains in 200 mg/dL of glucose were maintained at the highest optimal density after 48 h and the best viability of both strains were retained in the same glucose condition at 72 h. Furthermore, adhesion enhancement of H. pylori was significantly higher in 200 mg/dL of glucose as compared to that in 100 and 150 mg/dL (p < 0.05). CagA protein also increased in higher glucose condition. The cell-associated CagA and phosphorylated-CagA was significantly increased in 150 and 200 mg/dL of glucose concentrations as compared to that of 100 mg/dL (p < 0.05), which were found to be dose-dependent. CONCLUSION: Higher glucose could maintain H. pylori growth and viability after 48 h. H. pylori adhesion and CagA increased to further facilitate the enhancement of cell-associated CagA and phosphorylated CagA in higher glucose conditions. BioMed Central 2014-10-09 /pmc/articles/PMC4196111/ /pubmed/25296847 http://dx.doi.org/10.1186/s12929-014-0096-9 Text en © Sheu et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Sheu, Shew-Meei
Cheng, Hsin
Kao, Cheng-Ye
Yang, Yao-Jong
Wu, Jiunn-Jong
Sheu, Bor-Shyang
Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells
title Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells
title_full Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells
title_fullStr Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells
title_full_unstemmed Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells
title_short Higher glucose level can enhance the H. pylori adhesion and virulence related with type IV secretion system in AGS cells
title_sort higher glucose level can enhance the h. pylori adhesion and virulence related with type iv secretion system in ags cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196111/
https://www.ncbi.nlm.nih.gov/pubmed/25296847
http://dx.doi.org/10.1186/s12929-014-0096-9
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