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Genomic Evidence for a Globally Distributed, Bimodal Population in the Ovine Footrot Pathogen Dichelobacter nodosus
Footrot is a contagious, debilitating disease of sheep, causing major economic losses in most sheep-producing countries. The causative agent is the Gram-negative anaerobe Dichelobacter nodosus. Depending on the virulence of the infective bacterial strain, clinical signs vary from a mild interdigital...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196234/ https://www.ncbi.nlm.nih.gov/pubmed/25271288 http://dx.doi.org/10.1128/mBio.01821-14 |
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author | Kennan, Ruth M. Gilhuus, Marianne Frosth, Sara Seemann, Torsten Dhungyel, Om P. Whittington, Richard J. Boyce, John D. Powell, David R. Aspán, Anna Jørgensen, Hannah J. Bulach, Dieter M. Rood, Julian I. |
author_facet | Kennan, Ruth M. Gilhuus, Marianne Frosth, Sara Seemann, Torsten Dhungyel, Om P. Whittington, Richard J. Boyce, John D. Powell, David R. Aspán, Anna Jørgensen, Hannah J. Bulach, Dieter M. Rood, Julian I. |
author_sort | Kennan, Ruth M. |
collection | PubMed |
description | Footrot is a contagious, debilitating disease of sheep, causing major economic losses in most sheep-producing countries. The causative agent is the Gram-negative anaerobe Dichelobacter nodosus. Depending on the virulence of the infective bacterial strain, clinical signs vary from a mild interdigital dermatitis (benign footrot) to severe underrunning of the horn of the hoof (virulent footrot). The aim of this study was to investigate the genetic relationship between D. nodosus strains of different phenotypic virulences and between isolates from different geographic regions. Genome sequencing was performed on 103 D. nodosus isolates from eight different countries. Comparison of these genome sequences revealed that they were highly conserved, with >95% sequence identity. However, single nucleotide polymorphism analysis of the 31,627 nucleotides that were found to differ in one or more of the 103 sequenced isolates divided them into two distinct clades. Remarkably, this division correlated with known virulent and benign phenotypes, as well as with the single amino acid difference between the AprV2 and AprB2 proteases, which are produced by virulent and benign strains, respectively. This division was irrespective of the geographic origin of the isolates. However, within one of these clades, isolates from different geographic regions generally belonged to separate clusters. In summary, we have shown that D. nodosus has a bimodal population structure that is globally conserved and provide evidence that virulent and benign isolates represent two distinct forms of D. nodosus strains. These data have the potential to improve the diagnosis and targeted control of this economically significant disease. |
format | Online Article Text |
id | pubmed-4196234 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-41962342014-10-24 Genomic Evidence for a Globally Distributed, Bimodal Population in the Ovine Footrot Pathogen Dichelobacter nodosus Kennan, Ruth M. Gilhuus, Marianne Frosth, Sara Seemann, Torsten Dhungyel, Om P. Whittington, Richard J. Boyce, John D. Powell, David R. Aspán, Anna Jørgensen, Hannah J. Bulach, Dieter M. Rood, Julian I. mBio Research Article Footrot is a contagious, debilitating disease of sheep, causing major economic losses in most sheep-producing countries. The causative agent is the Gram-negative anaerobe Dichelobacter nodosus. Depending on the virulence of the infective bacterial strain, clinical signs vary from a mild interdigital dermatitis (benign footrot) to severe underrunning of the horn of the hoof (virulent footrot). The aim of this study was to investigate the genetic relationship between D. nodosus strains of different phenotypic virulences and between isolates from different geographic regions. Genome sequencing was performed on 103 D. nodosus isolates from eight different countries. Comparison of these genome sequences revealed that they were highly conserved, with >95% sequence identity. However, single nucleotide polymorphism analysis of the 31,627 nucleotides that were found to differ in one or more of the 103 sequenced isolates divided them into two distinct clades. Remarkably, this division correlated with known virulent and benign phenotypes, as well as with the single amino acid difference between the AprV2 and AprB2 proteases, which are produced by virulent and benign strains, respectively. This division was irrespective of the geographic origin of the isolates. However, within one of these clades, isolates from different geographic regions generally belonged to separate clusters. In summary, we have shown that D. nodosus has a bimodal population structure that is globally conserved and provide evidence that virulent and benign isolates represent two distinct forms of D. nodosus strains. These data have the potential to improve the diagnosis and targeted control of this economically significant disease. American Society for Microbiology 2014-09-30 /pmc/articles/PMC4196234/ /pubmed/25271288 http://dx.doi.org/10.1128/mBio.01821-14 Text en Copyright © 2014 Kennan et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kennan, Ruth M. Gilhuus, Marianne Frosth, Sara Seemann, Torsten Dhungyel, Om P. Whittington, Richard J. Boyce, John D. Powell, David R. Aspán, Anna Jørgensen, Hannah J. Bulach, Dieter M. Rood, Julian I. Genomic Evidence for a Globally Distributed, Bimodal Population in the Ovine Footrot Pathogen Dichelobacter nodosus |
title | Genomic Evidence for a Globally Distributed, Bimodal Population in the Ovine Footrot Pathogen Dichelobacter nodosus |
title_full | Genomic Evidence for a Globally Distributed, Bimodal Population in the Ovine Footrot Pathogen Dichelobacter nodosus |
title_fullStr | Genomic Evidence for a Globally Distributed, Bimodal Population in the Ovine Footrot Pathogen Dichelobacter nodosus |
title_full_unstemmed | Genomic Evidence for a Globally Distributed, Bimodal Population in the Ovine Footrot Pathogen Dichelobacter nodosus |
title_short | Genomic Evidence for a Globally Distributed, Bimodal Population in the Ovine Footrot Pathogen Dichelobacter nodosus |
title_sort | genomic evidence for a globally distributed, bimodal population in the ovine footrot pathogen dichelobacter nodosus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196234/ https://www.ncbi.nlm.nih.gov/pubmed/25271288 http://dx.doi.org/10.1128/mBio.01821-14 |
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