Cargando…

Islet amyloid with macrophage migration correlates with augmented β-cell deficits in type 2 diabetic patients

AIMS: Islet amyloid is a hallmark in type 2 diabetic subjects, but its implication in clinical features and development of islet pathology is still unclear. METHODS: From 118 autopsy cases with type 2 diabetes, 26 cases with islet amyloid deposition (DA+) were selected. Twenty diabetic subjects with...

Descripción completa

Detalles Bibliográficos
Autores principales: Kamata, Kosuke, Mizukami, Hiroki, Inaba, Wataru, Tsuboi, Kentaro, Tateishi, Yoshinori, Yoshida, Taro, Yagihashi, Soroku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Informa UK Ltd. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196506/
https://www.ncbi.nlm.nih.gov/pubmed/25007035
http://dx.doi.org/10.3109/13506129.2014.937857
_version_ 1782339488122404864
author Kamata, Kosuke
Mizukami, Hiroki
Inaba, Wataru
Tsuboi, Kentaro
Tateishi, Yoshinori
Yoshida, Taro
Yagihashi, Soroku
author_facet Kamata, Kosuke
Mizukami, Hiroki
Inaba, Wataru
Tsuboi, Kentaro
Tateishi, Yoshinori
Yoshida, Taro
Yagihashi, Soroku
author_sort Kamata, Kosuke
collection PubMed
description AIMS: Islet amyloid is a hallmark in type 2 diabetic subjects, but its implication in clinical features and development of islet pathology is still unclear. METHODS: From 118 autopsy cases with type 2 diabetes, 26 cases with islet amyloid deposition (DA+) were selected. Twenty diabetic subjects without obvious amyloid deposition (DA−) matched for the age and diabetes duration and 20 non-diabetic subjects (ND) served for comparison. We examined the severity of amyloid deposition and its relationships with population of endocrine cells, expression of cell damage markers or macrophage infiltration. Correlation of clinical profile with islet pathology was also sought on the subset of the investigated patients. RESULTS: β-Cell volume density was nearly 40% less in DA+ and 20% less in DA− when compared to ND. Severity of amyloid deposition correlated with reduced volume densities of β-cell and α-cell, and increased body mass index (BMI), but not with duration of diabetes, age or HbA1c. Amyloid-rich islets contained an increased number of macrophages mixed with β-cells with oxidative stress-related DNA damage, characterized by γH2AX expression, and suppressed (pro)insulin mRNA expression. CONCLUSIONS: In Japanese type 2 diabetic patients, islet amyloid was more common with severe β-cell loss and high BMI, associated with macrophage infiltration.
format Online
Article
Text
id pubmed-4196506
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Informa UK Ltd.
record_format MEDLINE/PubMed
spelling pubmed-41965062014-10-27 Islet amyloid with macrophage migration correlates with augmented β-cell deficits in type 2 diabetic patients Kamata, Kosuke Mizukami, Hiroki Inaba, Wataru Tsuboi, Kentaro Tateishi, Yoshinori Yoshida, Taro Yagihashi, Soroku Amyloid Original Article AIMS: Islet amyloid is a hallmark in type 2 diabetic subjects, but its implication in clinical features and development of islet pathology is still unclear. METHODS: From 118 autopsy cases with type 2 diabetes, 26 cases with islet amyloid deposition (DA+) were selected. Twenty diabetic subjects without obvious amyloid deposition (DA−) matched for the age and diabetes duration and 20 non-diabetic subjects (ND) served for comparison. We examined the severity of amyloid deposition and its relationships with population of endocrine cells, expression of cell damage markers or macrophage infiltration. Correlation of clinical profile with islet pathology was also sought on the subset of the investigated patients. RESULTS: β-Cell volume density was nearly 40% less in DA+ and 20% less in DA− when compared to ND. Severity of amyloid deposition correlated with reduced volume densities of β-cell and α-cell, and increased body mass index (BMI), but not with duration of diabetes, age or HbA1c. Amyloid-rich islets contained an increased number of macrophages mixed with β-cells with oxidative stress-related DNA damage, characterized by γH2AX expression, and suppressed (pro)insulin mRNA expression. CONCLUSIONS: In Japanese type 2 diabetic patients, islet amyloid was more common with severe β-cell loss and high BMI, associated with macrophage infiltration. Informa UK Ltd. 2014-09 2014-07-09 /pmc/articles/PMC4196506/ /pubmed/25007035 http://dx.doi.org/10.3109/13506129.2014.937857 Text en © 2014 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the source is credited.
spellingShingle Original Article
Kamata, Kosuke
Mizukami, Hiroki
Inaba, Wataru
Tsuboi, Kentaro
Tateishi, Yoshinori
Yoshida, Taro
Yagihashi, Soroku
Islet amyloid with macrophage migration correlates with augmented β-cell deficits in type 2 diabetic patients
title Islet amyloid with macrophage migration correlates with augmented β-cell deficits in type 2 diabetic patients
title_full Islet amyloid with macrophage migration correlates with augmented β-cell deficits in type 2 diabetic patients
title_fullStr Islet amyloid with macrophage migration correlates with augmented β-cell deficits in type 2 diabetic patients
title_full_unstemmed Islet amyloid with macrophage migration correlates with augmented β-cell deficits in type 2 diabetic patients
title_short Islet amyloid with macrophage migration correlates with augmented β-cell deficits in type 2 diabetic patients
title_sort islet amyloid with macrophage migration correlates with augmented β-cell deficits in type 2 diabetic patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196506/
https://www.ncbi.nlm.nih.gov/pubmed/25007035
http://dx.doi.org/10.3109/13506129.2014.937857
work_keys_str_mv AT kamatakosuke isletamyloidwithmacrophagemigrationcorrelateswithaugmentedbcelldeficitsintype2diabeticpatients
AT mizukamihiroki isletamyloidwithmacrophagemigrationcorrelateswithaugmentedbcelldeficitsintype2diabeticpatients
AT inabawataru isletamyloidwithmacrophagemigrationcorrelateswithaugmentedbcelldeficitsintype2diabeticpatients
AT tsuboikentaro isletamyloidwithmacrophagemigrationcorrelateswithaugmentedbcelldeficitsintype2diabeticpatients
AT tateishiyoshinori isletamyloidwithmacrophagemigrationcorrelateswithaugmentedbcelldeficitsintype2diabeticpatients
AT yoshidataro isletamyloidwithmacrophagemigrationcorrelateswithaugmentedbcelldeficitsintype2diabeticpatients
AT yagihashisoroku isletamyloidwithmacrophagemigrationcorrelateswithaugmentedbcelldeficitsintype2diabeticpatients