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miRNA-940 reduction contributes to human Tetralogy of Fallot development

Tetralogy of Fallot (TOF) is a complex congenital heart defect and the microRNAs regulation in TOF development is largely unknown. Herein, we explored the role of miRNAs in TOF. Among 75 dysregulated miRNAs identified from human heart tissues, miRNA-940 was the most down-regulated one. Interestingly...

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Autores principales: Liang, Dandan, Xu, Xinran, Deng, Fangfei, Feng, Jing, Zhang, Hong, Liu, Ying, Zhang, Yangyang, Pan, Lei, Liu, Yi, Zhang, Dasheng, Li, Jun, Liang, Xingqun, Sun, Yunfu, Xiao, Junjie, Chen, Yi-Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196658/
https://www.ncbi.nlm.nih.gov/pubmed/24889693
http://dx.doi.org/10.1111/jcmm.12309
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author Liang, Dandan
Xu, Xinran
Deng, Fangfei
Feng, Jing
Zhang, Hong
Liu, Ying
Zhang, Yangyang
Pan, Lei
Liu, Yi
Zhang, Dasheng
Li, Jun
Liang, Xingqun
Sun, Yunfu
Xiao, Junjie
Chen, Yi-Han
author_facet Liang, Dandan
Xu, Xinran
Deng, Fangfei
Feng, Jing
Zhang, Hong
Liu, Ying
Zhang, Yangyang
Pan, Lei
Liu, Yi
Zhang, Dasheng
Li, Jun
Liang, Xingqun
Sun, Yunfu
Xiao, Junjie
Chen, Yi-Han
author_sort Liang, Dandan
collection PubMed
description Tetralogy of Fallot (TOF) is a complex congenital heart defect and the microRNAs regulation in TOF development is largely unknown. Herein, we explored the role of miRNAs in TOF. Among 75 dysregulated miRNAs identified from human heart tissues, miRNA-940 was the most down-regulated one. Interestingly, miRNA-940 was most highly expressed in normal human right ventricular out-flow tract comparing to other heart chambers. As TOF is caused by altered proliferation, migration and/or differentiation of the progenitor cells of the secondary heart field, we isolated Sca-1(+) human cardiomyocyte progenitor cells (hCMPC) for miRNA-940 function analysis. miRNA-940 reduction significantly promoted hCMPCs proliferation and inhibited hCMPCs migration. We found that JARID2 is an endogenous target regulated by miRNA-940. Functional analyses showed that JARID2 also affected hCMPCs proliferation and migration. Thus, decreased miRNA-940 affects the proliferation and migration of the progenitor cells of the secondary heart field by targeting JARID2 and potentially leads to TOF development.
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spelling pubmed-41966582014-12-03 miRNA-940 reduction contributes to human Tetralogy of Fallot development Liang, Dandan Xu, Xinran Deng, Fangfei Feng, Jing Zhang, Hong Liu, Ying Zhang, Yangyang Pan, Lei Liu, Yi Zhang, Dasheng Li, Jun Liang, Xingqun Sun, Yunfu Xiao, Junjie Chen, Yi-Han J Cell Mol Med Original Articles Tetralogy of Fallot (TOF) is a complex congenital heart defect and the microRNAs regulation in TOF development is largely unknown. Herein, we explored the role of miRNAs in TOF. Among 75 dysregulated miRNAs identified from human heart tissues, miRNA-940 was the most down-regulated one. Interestingly, miRNA-940 was most highly expressed in normal human right ventricular out-flow tract comparing to other heart chambers. As TOF is caused by altered proliferation, migration and/or differentiation of the progenitor cells of the secondary heart field, we isolated Sca-1(+) human cardiomyocyte progenitor cells (hCMPC) for miRNA-940 function analysis. miRNA-940 reduction significantly promoted hCMPCs proliferation and inhibited hCMPCs migration. We found that JARID2 is an endogenous target regulated by miRNA-940. Functional analyses showed that JARID2 also affected hCMPCs proliferation and migration. Thus, decreased miRNA-940 affects the proliferation and migration of the progenitor cells of the secondary heart field by targeting JARID2 and potentially leads to TOF development. Blackwell Publishing Ltd 2014-09 2014-06-01 /pmc/articles/PMC4196658/ /pubmed/24889693 http://dx.doi.org/10.1111/jcmm.12309 Text en © 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Liang, Dandan
Xu, Xinran
Deng, Fangfei
Feng, Jing
Zhang, Hong
Liu, Ying
Zhang, Yangyang
Pan, Lei
Liu, Yi
Zhang, Dasheng
Li, Jun
Liang, Xingqun
Sun, Yunfu
Xiao, Junjie
Chen, Yi-Han
miRNA-940 reduction contributes to human Tetralogy of Fallot development
title miRNA-940 reduction contributes to human Tetralogy of Fallot development
title_full miRNA-940 reduction contributes to human Tetralogy of Fallot development
title_fullStr miRNA-940 reduction contributes to human Tetralogy of Fallot development
title_full_unstemmed miRNA-940 reduction contributes to human Tetralogy of Fallot development
title_short miRNA-940 reduction contributes to human Tetralogy of Fallot development
title_sort mirna-940 reduction contributes to human tetralogy of fallot development
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196658/
https://www.ncbi.nlm.nih.gov/pubmed/24889693
http://dx.doi.org/10.1111/jcmm.12309
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