Cargando…
miRNA-940 reduction contributes to human Tetralogy of Fallot development
Tetralogy of Fallot (TOF) is a complex congenital heart defect and the microRNAs regulation in TOF development is largely unknown. Herein, we explored the role of miRNAs in TOF. Among 75 dysregulated miRNAs identified from human heart tissues, miRNA-940 was the most down-regulated one. Interestingly...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196658/ https://www.ncbi.nlm.nih.gov/pubmed/24889693 http://dx.doi.org/10.1111/jcmm.12309 |
_version_ | 1782339512598265856 |
---|---|
author | Liang, Dandan Xu, Xinran Deng, Fangfei Feng, Jing Zhang, Hong Liu, Ying Zhang, Yangyang Pan, Lei Liu, Yi Zhang, Dasheng Li, Jun Liang, Xingqun Sun, Yunfu Xiao, Junjie Chen, Yi-Han |
author_facet | Liang, Dandan Xu, Xinran Deng, Fangfei Feng, Jing Zhang, Hong Liu, Ying Zhang, Yangyang Pan, Lei Liu, Yi Zhang, Dasheng Li, Jun Liang, Xingqun Sun, Yunfu Xiao, Junjie Chen, Yi-Han |
author_sort | Liang, Dandan |
collection | PubMed |
description | Tetralogy of Fallot (TOF) is a complex congenital heart defect and the microRNAs regulation in TOF development is largely unknown. Herein, we explored the role of miRNAs in TOF. Among 75 dysregulated miRNAs identified from human heart tissues, miRNA-940 was the most down-regulated one. Interestingly, miRNA-940 was most highly expressed in normal human right ventricular out-flow tract comparing to other heart chambers. As TOF is caused by altered proliferation, migration and/or differentiation of the progenitor cells of the secondary heart field, we isolated Sca-1(+) human cardiomyocyte progenitor cells (hCMPC) for miRNA-940 function analysis. miRNA-940 reduction significantly promoted hCMPCs proliferation and inhibited hCMPCs migration. We found that JARID2 is an endogenous target regulated by miRNA-940. Functional analyses showed that JARID2 also affected hCMPCs proliferation and migration. Thus, decreased miRNA-940 affects the proliferation and migration of the progenitor cells of the secondary heart field by targeting JARID2 and potentially leads to TOF development. |
format | Online Article Text |
id | pubmed-4196658 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-41966582014-12-03 miRNA-940 reduction contributes to human Tetralogy of Fallot development Liang, Dandan Xu, Xinran Deng, Fangfei Feng, Jing Zhang, Hong Liu, Ying Zhang, Yangyang Pan, Lei Liu, Yi Zhang, Dasheng Li, Jun Liang, Xingqun Sun, Yunfu Xiao, Junjie Chen, Yi-Han J Cell Mol Med Original Articles Tetralogy of Fallot (TOF) is a complex congenital heart defect and the microRNAs regulation in TOF development is largely unknown. Herein, we explored the role of miRNAs in TOF. Among 75 dysregulated miRNAs identified from human heart tissues, miRNA-940 was the most down-regulated one. Interestingly, miRNA-940 was most highly expressed in normal human right ventricular out-flow tract comparing to other heart chambers. As TOF is caused by altered proliferation, migration and/or differentiation of the progenitor cells of the secondary heart field, we isolated Sca-1(+) human cardiomyocyte progenitor cells (hCMPC) for miRNA-940 function analysis. miRNA-940 reduction significantly promoted hCMPCs proliferation and inhibited hCMPCs migration. We found that JARID2 is an endogenous target regulated by miRNA-940. Functional analyses showed that JARID2 also affected hCMPCs proliferation and migration. Thus, decreased miRNA-940 affects the proliferation and migration of the progenitor cells of the secondary heart field by targeting JARID2 and potentially leads to TOF development. Blackwell Publishing Ltd 2014-09 2014-06-01 /pmc/articles/PMC4196658/ /pubmed/24889693 http://dx.doi.org/10.1111/jcmm.12309 Text en © 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Liang, Dandan Xu, Xinran Deng, Fangfei Feng, Jing Zhang, Hong Liu, Ying Zhang, Yangyang Pan, Lei Liu, Yi Zhang, Dasheng Li, Jun Liang, Xingqun Sun, Yunfu Xiao, Junjie Chen, Yi-Han miRNA-940 reduction contributes to human Tetralogy of Fallot development |
title | miRNA-940 reduction contributes to human Tetralogy of Fallot development |
title_full | miRNA-940 reduction contributes to human Tetralogy of Fallot development |
title_fullStr | miRNA-940 reduction contributes to human Tetralogy of Fallot development |
title_full_unstemmed | miRNA-940 reduction contributes to human Tetralogy of Fallot development |
title_short | miRNA-940 reduction contributes to human Tetralogy of Fallot development |
title_sort | mirna-940 reduction contributes to human tetralogy of fallot development |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196658/ https://www.ncbi.nlm.nih.gov/pubmed/24889693 http://dx.doi.org/10.1111/jcmm.12309 |
work_keys_str_mv | AT liangdandan mirna940reductioncontributestohumantetralogyoffallotdevelopment AT xuxinran mirna940reductioncontributestohumantetralogyoffallotdevelopment AT dengfangfei mirna940reductioncontributestohumantetralogyoffallotdevelopment AT fengjing mirna940reductioncontributestohumantetralogyoffallotdevelopment AT zhanghong mirna940reductioncontributestohumantetralogyoffallotdevelopment AT liuying mirna940reductioncontributestohumantetralogyoffallotdevelopment AT zhangyangyang mirna940reductioncontributestohumantetralogyoffallotdevelopment AT panlei mirna940reductioncontributestohumantetralogyoffallotdevelopment AT liuyi mirna940reductioncontributestohumantetralogyoffallotdevelopment AT zhangdasheng mirna940reductioncontributestohumantetralogyoffallotdevelopment AT lijun mirna940reductioncontributestohumantetralogyoffallotdevelopment AT liangxingqun mirna940reductioncontributestohumantetralogyoffallotdevelopment AT sunyunfu mirna940reductioncontributestohumantetralogyoffallotdevelopment AT xiaojunjie mirna940reductioncontributestohumantetralogyoffallotdevelopment AT chenyihan mirna940reductioncontributestohumantetralogyoffallotdevelopment |