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Identification of a Novel Nucleocytoplasmic Shuttling RNA Helicase of Trypanosomes
Gene expression in trypanosomes is controlled mostly by post-transcriptional pathways. Little is known about the components of mRNA nucleocytoplasmic export routes in these parasites. Comparative genomics has shown that the mRNA transport pathway is the least conserved pathway among eukaryotes. None...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196910/ https://www.ncbi.nlm.nih.gov/pubmed/25313564 http://dx.doi.org/10.1371/journal.pone.0109521 |
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author | Inoue, Alexandre Haruo Serpeloni, Mariana Hiraiwa, Priscila Mazzocchi Yamada-Ogatta, Sueli Fumie Muniz, João Renato Carvalho Motta, Maria Cristina Machado Vidal, Newton Medeiros Goldenberg, Samuel Ávila, Andréa Rodrigues |
author_facet | Inoue, Alexandre Haruo Serpeloni, Mariana Hiraiwa, Priscila Mazzocchi Yamada-Ogatta, Sueli Fumie Muniz, João Renato Carvalho Motta, Maria Cristina Machado Vidal, Newton Medeiros Goldenberg, Samuel Ávila, Andréa Rodrigues |
author_sort | Inoue, Alexandre Haruo |
collection | PubMed |
description | Gene expression in trypanosomes is controlled mostly by post-transcriptional pathways. Little is known about the components of mRNA nucleocytoplasmic export routes in these parasites. Comparative genomics has shown that the mRNA transport pathway is the least conserved pathway among eukaryotes. Nonetheless, we identified a RNA helicase (Hel45) that is conserved across eukaryotes and similar to shuttling proteins involved in mRNA export. We used in silico analysis to predict the structure of Trypanosoma cruzi Hel45, including the N-terminal domain and the C-terminal domain, and our findings suggest that this RNA helicase can form complexes with mRNA. Hel45 was present in both nucleus and cytoplasm. Electron microscopy showed that Hel45 is clustered close to the cytoplasmic side of nuclear pore complexes, and is also present in the nucleus where it is associated with peripheral compact chromatin. Deletion of a predicted Nuclear Export Signal motif led to the accumulation of Hel45ΔNES in the nucleus, indicating that Hel45 shuttles between the nucleus and the cytoplasm. This transport was dependent on active transcription but did not depend on the exportin Crm1. Knockdown of Mex67 in T. brucei caused the nuclear accumulation of the T. brucei ortholog of Hel45. Indeed, Hel45 is present in mRNA ribonucleoprotein complexes that are not associated with polysomes. It is still necessary to confirm the precise function of Hel45. However, this RNA helicase is associated with mRNA metabolism and its nucleocytoplasmic shuttling is dependent on an mRNA export route involving Mex67 receptor. |
format | Online Article Text |
id | pubmed-4196910 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41969102014-10-16 Identification of a Novel Nucleocytoplasmic Shuttling RNA Helicase of Trypanosomes Inoue, Alexandre Haruo Serpeloni, Mariana Hiraiwa, Priscila Mazzocchi Yamada-Ogatta, Sueli Fumie Muniz, João Renato Carvalho Motta, Maria Cristina Machado Vidal, Newton Medeiros Goldenberg, Samuel Ávila, Andréa Rodrigues PLoS One Research Article Gene expression in trypanosomes is controlled mostly by post-transcriptional pathways. Little is known about the components of mRNA nucleocytoplasmic export routes in these parasites. Comparative genomics has shown that the mRNA transport pathway is the least conserved pathway among eukaryotes. Nonetheless, we identified a RNA helicase (Hel45) that is conserved across eukaryotes and similar to shuttling proteins involved in mRNA export. We used in silico analysis to predict the structure of Trypanosoma cruzi Hel45, including the N-terminal domain and the C-terminal domain, and our findings suggest that this RNA helicase can form complexes with mRNA. Hel45 was present in both nucleus and cytoplasm. Electron microscopy showed that Hel45 is clustered close to the cytoplasmic side of nuclear pore complexes, and is also present in the nucleus where it is associated with peripheral compact chromatin. Deletion of a predicted Nuclear Export Signal motif led to the accumulation of Hel45ΔNES in the nucleus, indicating that Hel45 shuttles between the nucleus and the cytoplasm. This transport was dependent on active transcription but did not depend on the exportin Crm1. Knockdown of Mex67 in T. brucei caused the nuclear accumulation of the T. brucei ortholog of Hel45. Indeed, Hel45 is present in mRNA ribonucleoprotein complexes that are not associated with polysomes. It is still necessary to confirm the precise function of Hel45. However, this RNA helicase is associated with mRNA metabolism and its nucleocytoplasmic shuttling is dependent on an mRNA export route involving Mex67 receptor. Public Library of Science 2014-10-14 /pmc/articles/PMC4196910/ /pubmed/25313564 http://dx.doi.org/10.1371/journal.pone.0109521 Text en © 2014 Inoue et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Inoue, Alexandre Haruo Serpeloni, Mariana Hiraiwa, Priscila Mazzocchi Yamada-Ogatta, Sueli Fumie Muniz, João Renato Carvalho Motta, Maria Cristina Machado Vidal, Newton Medeiros Goldenberg, Samuel Ávila, Andréa Rodrigues Identification of a Novel Nucleocytoplasmic Shuttling RNA Helicase of Trypanosomes |
title | Identification of a Novel Nucleocytoplasmic Shuttling RNA Helicase of Trypanosomes |
title_full | Identification of a Novel Nucleocytoplasmic Shuttling RNA Helicase of Trypanosomes |
title_fullStr | Identification of a Novel Nucleocytoplasmic Shuttling RNA Helicase of Trypanosomes |
title_full_unstemmed | Identification of a Novel Nucleocytoplasmic Shuttling RNA Helicase of Trypanosomes |
title_short | Identification of a Novel Nucleocytoplasmic Shuttling RNA Helicase of Trypanosomes |
title_sort | identification of a novel nucleocytoplasmic shuttling rna helicase of trypanosomes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196910/ https://www.ncbi.nlm.nih.gov/pubmed/25313564 http://dx.doi.org/10.1371/journal.pone.0109521 |
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