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MiR-199a Regulates Cell Proliferation and Survival by Targeting FZD7

A growing amount of evidence indicates that miRNAs are important regulators of multiple cellular processes and, when expressed aberrantly in different types of cancer such as hepatocellular carcinoma (HCC), play significant roles in tumorigenesis and progression. Aberrant expression of miR-199a-5p (...

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Autores principales: Song, Jiugang, Gao, Liucun, Yang, Guang, Tang, Shanhong, Xie, Huahong, Wang, Yongji, Wang, Jingbo, Zhang, Yanping, Jin, Jiang, Gou, Yawen, Yang, Zhiping, Chen, Zheng, Wu, Kaichun, Liu, Jie, Fan, Daiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196968/
https://www.ncbi.nlm.nih.gov/pubmed/25313882
http://dx.doi.org/10.1371/journal.pone.0110074
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author Song, Jiugang
Gao, Liucun
Yang, Guang
Tang, Shanhong
Xie, Huahong
Wang, Yongji
Wang, Jingbo
Zhang, Yanping
Jin, Jiang
Gou, Yawen
Yang, Zhiping
Chen, Zheng
Wu, Kaichun
Liu, Jie
Fan, Daiming
author_facet Song, Jiugang
Gao, Liucun
Yang, Guang
Tang, Shanhong
Xie, Huahong
Wang, Yongji
Wang, Jingbo
Zhang, Yanping
Jin, Jiang
Gou, Yawen
Yang, Zhiping
Chen, Zheng
Wu, Kaichun
Liu, Jie
Fan, Daiming
author_sort Song, Jiugang
collection PubMed
description A growing amount of evidence indicates that miRNAs are important regulators of multiple cellular processes and, when expressed aberrantly in different types of cancer such as hepatocellular carcinoma (HCC), play significant roles in tumorigenesis and progression. Aberrant expression of miR-199a-5p (also called miR-199a) was found to contribute to carcinogenesis in different types of cancer, including HCC. However, the precise molecular mechanism is not yet fully understood. The present study showed that miR-199a is frequently down-regulated in HCC tissues and cells. Importantly, lower expression of miR-199a was significantly correlated with the malignant potential and poor prognosis of HCC, and restoration of miR-199a in HCC cells led to inhibition of the cell proliferation and cell cycle in vitro and in vivo. Furthermore, Frizzled type 7 receptor (FZD7), the most important Wnt receptor involved in cancer development and progression, was identified as a functional target of miR-199a. In addition, these findings were further strengthened by results showing that expression of FZD7 was inversely correlated with miR-199a in both HCC tissues and cells and that over-expression of miR-199a could significantly down-regulate the expression of genes downstream of FZD7, including β-catenin, Jun, Cyclin D1 and Myc. In conclusion, these findings not only help us to better elucidate the molecular mechanisms of hepatocarcinogenesis from a fresh perspective but also provide a new theoretical basis to further investigate miR-199a as a potential biomarker and a promising approach for HCC treatment.
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spelling pubmed-41969682014-10-16 MiR-199a Regulates Cell Proliferation and Survival by Targeting FZD7 Song, Jiugang Gao, Liucun Yang, Guang Tang, Shanhong Xie, Huahong Wang, Yongji Wang, Jingbo Zhang, Yanping Jin, Jiang Gou, Yawen Yang, Zhiping Chen, Zheng Wu, Kaichun Liu, Jie Fan, Daiming PLoS One Research Article A growing amount of evidence indicates that miRNAs are important regulators of multiple cellular processes and, when expressed aberrantly in different types of cancer such as hepatocellular carcinoma (HCC), play significant roles in tumorigenesis and progression. Aberrant expression of miR-199a-5p (also called miR-199a) was found to contribute to carcinogenesis in different types of cancer, including HCC. However, the precise molecular mechanism is not yet fully understood. The present study showed that miR-199a is frequently down-regulated in HCC tissues and cells. Importantly, lower expression of miR-199a was significantly correlated with the malignant potential and poor prognosis of HCC, and restoration of miR-199a in HCC cells led to inhibition of the cell proliferation and cell cycle in vitro and in vivo. Furthermore, Frizzled type 7 receptor (FZD7), the most important Wnt receptor involved in cancer development and progression, was identified as a functional target of miR-199a. In addition, these findings were further strengthened by results showing that expression of FZD7 was inversely correlated with miR-199a in both HCC tissues and cells and that over-expression of miR-199a could significantly down-regulate the expression of genes downstream of FZD7, including β-catenin, Jun, Cyclin D1 and Myc. In conclusion, these findings not only help us to better elucidate the molecular mechanisms of hepatocarcinogenesis from a fresh perspective but also provide a new theoretical basis to further investigate miR-199a as a potential biomarker and a promising approach for HCC treatment. Public Library of Science 2014-10-14 /pmc/articles/PMC4196968/ /pubmed/25313882 http://dx.doi.org/10.1371/journal.pone.0110074 Text en © 2014 Song et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Song, Jiugang
Gao, Liucun
Yang, Guang
Tang, Shanhong
Xie, Huahong
Wang, Yongji
Wang, Jingbo
Zhang, Yanping
Jin, Jiang
Gou, Yawen
Yang, Zhiping
Chen, Zheng
Wu, Kaichun
Liu, Jie
Fan, Daiming
MiR-199a Regulates Cell Proliferation and Survival by Targeting FZD7
title MiR-199a Regulates Cell Proliferation and Survival by Targeting FZD7
title_full MiR-199a Regulates Cell Proliferation and Survival by Targeting FZD7
title_fullStr MiR-199a Regulates Cell Proliferation and Survival by Targeting FZD7
title_full_unstemmed MiR-199a Regulates Cell Proliferation and Survival by Targeting FZD7
title_short MiR-199a Regulates Cell Proliferation and Survival by Targeting FZD7
title_sort mir-199a regulates cell proliferation and survival by targeting fzd7
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4196968/
https://www.ncbi.nlm.nih.gov/pubmed/25313882
http://dx.doi.org/10.1371/journal.pone.0110074
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