Tpz1(TPP)(1) SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association

Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with ov...

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Autores principales: Garg, Mansi, Gurung, Resham L, Mansoubi, Sahar, Ahmed, Jubed O, Davé, Anoushka, Watts, Felicity Z, Bianchi, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BlackWell Publishing Ltd 2014
Materias:
Scientific Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4197044/
https://www.ncbi.nlm.nih.gov/pubmed/24925530
http://dx.doi.org/10.15252/embr.201438919
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author Garg, Mansi
Gurung, Resham L
Mansoubi, Sahar
Ahmed, Jubed O
Davé, Anoushka
Watts, Felicity Z
Bianchi, Alessandro
author_facet Garg, Mansi
Gurung, Resham L
Mansoubi, Sahar
Ahmed, Jubed O
Davé, Anoushka
Watts, Felicity Z
Bianchi, Alessandro
author_sort Garg, Mansi
collection PubMed
description Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1/POT1. We show that the fission yeast TPP1 ortholog, Tpz1, is SUMOylated. Tpz1 SUMOylation restricts telomere elongation and promotes Stn1/Ten1 telomere association, and a SUMO-Tpz1 fusion protein has increased affinity for Stn1. Our data suggest that SUMO inhibits telomerase through stimulation of Stn1/Ten1 action by Tpz1, highlighting the evolutionary conservation of the regulation of CST function by SUMOylation.
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spelling pubmed-41970442015-08-01 Tpz1(TPP)(1) SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association Garg, Mansi Gurung, Resham L Mansoubi, Sahar Ahmed, Jubed O Davé, Anoushka Watts, Felicity Z Bianchi, Alessandro EMBO Rep Scientific Reports Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13 SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1/POT1. We show that the fission yeast TPP1 ortholog, Tpz1, is SUMOylated. Tpz1 SUMOylation restricts telomere elongation and promotes Stn1/Ten1 telomere association, and a SUMO-Tpz1 fusion protein has increased affinity for Stn1. Our data suggest that SUMO inhibits telomerase through stimulation of Stn1/Ten1 action by Tpz1, highlighting the evolutionary conservation of the regulation of CST function by SUMOylation. BlackWell Publishing Ltd 2014-08 2014-06-12 /pmc/articles/PMC4197044/ /pubmed/24925530 http://dx.doi.org/10.15252/embr.201438919 Text en © 2014 The Authors. Published under the terms of the CC BY 4.0 License http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Scientific Reports
Garg, Mansi
Gurung, Resham L
Mansoubi, Sahar
Ahmed, Jubed O
Davé, Anoushka
Watts, Felicity Z
Bianchi, Alessandro
Tpz1(TPP)(1) SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association
title Tpz1(TPP)(1) SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association
title_full Tpz1(TPP)(1) SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association
title_fullStr Tpz1(TPP)(1) SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association
title_full_unstemmed Tpz1(TPP)(1) SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association
title_short Tpz1(TPP)(1) SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association
title_sort tpz1(tpp)(1) sumoylation reveals evolutionary conservation of sumo-dependent stn1 telomere association
topic Scientific Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4197044/
https://www.ncbi.nlm.nih.gov/pubmed/24925530
http://dx.doi.org/10.15252/embr.201438919
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