Cargando…

Characterization of SET/I2PP2A Isoforms in Dogs

SET is an endogenous protein phosphatase 2A (PP2A) inhibitor and is associated with a poor prognosis in human leukemia. Previously, we reported increased SET protein levels in canine lymphoma cell lines and the potential therapeutic application of SET antagonists in canine lymphoma. Here, we found t...

Descripción completa

Detalles Bibliográficos
Autores principales: YABE, Ryotaro, FUJIWARA, Nobuyuki, MIZUNO, Takuya, USUI, Tatsuya, OHAMA, Takashi, SATO, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4197151/
https://www.ncbi.nlm.nih.gov/pubmed/24897959
http://dx.doi.org/10.1292/jvms.14-0209
_version_ 1782339568995926016
author YABE, Ryotaro
FUJIWARA, Nobuyuki
MIZUNO, Takuya
USUI, Tatsuya
OHAMA, Takashi
SATO, Koichi
author_facet YABE, Ryotaro
FUJIWARA, Nobuyuki
MIZUNO, Takuya
USUI, Tatsuya
OHAMA, Takashi
SATO, Koichi
author_sort YABE, Ryotaro
collection PubMed
description SET is an endogenous protein phosphatase 2A (PP2A) inhibitor and is associated with a poor prognosis in human leukemia. Previously, we reported increased SET protein levels in canine lymphoma cell lines and the potential therapeutic application of SET antagonists in canine lymphoma. Here, we found that canine cells express several isoforms of the SET protein. We cloned 4 isoforms of SET, named SETα, β, γ and δ. Genomic BLAST showed that the SET genes are located on chromosomes X, 7, 1 and 8, respectively. An immunofluorescent study showed nuclear localization of SETα and β, and nuclear and cytosolic localization of SETγ and δ. We confirmed that SETα and β possess the ability to associate with PP2A. Our data reveal the existence of unique SET isoforms that should be taken into account in SET-targeting drug development studies in dogs.
format Online
Article
Text
id pubmed-4197151
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-41971512014-10-17 Characterization of SET/I2PP2A Isoforms in Dogs YABE, Ryotaro FUJIWARA, Nobuyuki MIZUNO, Takuya USUI, Tatsuya OHAMA, Takashi SATO, Koichi J Vet Med Sci Pharmacology SET is an endogenous protein phosphatase 2A (PP2A) inhibitor and is associated with a poor prognosis in human leukemia. Previously, we reported increased SET protein levels in canine lymphoma cell lines and the potential therapeutic application of SET antagonists in canine lymphoma. Here, we found that canine cells express several isoforms of the SET protein. We cloned 4 isoforms of SET, named SETα, β, γ and δ. Genomic BLAST showed that the SET genes are located on chromosomes X, 7, 1 and 8, respectively. An immunofluorescent study showed nuclear localization of SETα and β, and nuclear and cytosolic localization of SETγ and δ. We confirmed that SETα and β possess the ability to associate with PP2A. Our data reveal the existence of unique SET isoforms that should be taken into account in SET-targeting drug development studies in dogs. The Japanese Society of Veterinary Science 2014-06-03 2014-09 /pmc/articles/PMC4197151/ /pubmed/24897959 http://dx.doi.org/10.1292/jvms.14-0209 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Pharmacology
YABE, Ryotaro
FUJIWARA, Nobuyuki
MIZUNO, Takuya
USUI, Tatsuya
OHAMA, Takashi
SATO, Koichi
Characterization of SET/I2PP2A Isoforms in Dogs
title Characterization of SET/I2PP2A Isoforms in Dogs
title_full Characterization of SET/I2PP2A Isoforms in Dogs
title_fullStr Characterization of SET/I2PP2A Isoforms in Dogs
title_full_unstemmed Characterization of SET/I2PP2A Isoforms in Dogs
title_short Characterization of SET/I2PP2A Isoforms in Dogs
title_sort characterization of set/i2pp2a isoforms in dogs
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4197151/
https://www.ncbi.nlm.nih.gov/pubmed/24897959
http://dx.doi.org/10.1292/jvms.14-0209
work_keys_str_mv AT yaberyotaro characterizationofseti2pp2aisoformsindogs
AT fujiwaranobuyuki characterizationofseti2pp2aisoformsindogs
AT mizunotakuya characterizationofseti2pp2aisoformsindogs
AT usuitatsuya characterizationofseti2pp2aisoformsindogs
AT ohamatakashi characterizationofseti2pp2aisoformsindogs
AT satokoichi characterizationofseti2pp2aisoformsindogs