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Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma

BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been r...

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Autores principales: Shen, Cong-Cong, Kang, Yu-Huan, Yu, Lin, Cui, Dan-Dan, He, Yi, Yang, Jin-Liang, Gou, Lan-Tu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4197282/
https://www.ncbi.nlm.nih.gov/pubmed/25418358
http://dx.doi.org/10.1186/0717-6287-47-52
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author Shen, Cong-Cong
Kang, Yu-Huan
Yu, Lin
Cui, Dan-Dan
He, Yi
Yang, Jin-Liang
Gou, Lan-Tu
author_facet Shen, Cong-Cong
Kang, Yu-Huan
Yu, Lin
Cui, Dan-Dan
He, Yi
Yang, Jin-Liang
Gou, Lan-Tu
author_sort Shen, Cong-Cong
collection PubMed
description BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed. RESULTS: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101. CONCLUSIONS: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions.
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spelling pubmed-41972822014-10-16 Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma Shen, Cong-Cong Kang, Yu-Huan Yu, Lin Cui, Dan-Dan He, Yi Yang, Jin-Liang Gou, Lan-Tu Biol Res Research Article BACKGROUND: Testis-expressed sequence 101 (TEX101) was found to be highly expressed in testis and involved in acrosome reaction in previous studies. Recently, the metastasis suppressor function of TEX101 in cancer was disclosed, but the comprehensive investigation of its expression has rarely been reported. In this study, the expression features of TEX101 in normal human organs and seminoma were systematically analyzed. RESULTS: Immunohistochemistry demonstrated intense staining of TEX101 in human testis tissues; however, its expression in 27 other types of normal human organs, including the ovary, was negligible. Higher expression of TEX101 was observed in the spermatocytes and spermatids of the testis, but relatively lower staining was detected in spermatogonia. Western blotting showed a single TEX101 band of 38 kDa in human testis, but it did not correspond to the predicted molecular weight of its mature form at 21 KDa. Furthermore, we examined seminoma tissues by immunohistochemistry and found that none of the 36 samples expressed TEX101. CONCLUSIONS: Our data confirmed TEX101 to be a testis protein that could be related to the maturation process of male germ cells. The lack of TEX101 in seminoma indicated its potential role in tumor progression. This characteristic expression of TEX101 could provide a valuable reference for understanding its biological functions. BioMed Central 2014-10-01 /pmc/articles/PMC4197282/ /pubmed/25418358 http://dx.doi.org/10.1186/0717-6287-47-52 Text en © Shen et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Shen, Cong-Cong
Kang, Yu-Huan
Yu, Lin
Cui, Dan-Dan
He, Yi
Yang, Jin-Liang
Gou, Lan-Tu
Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
title Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
title_full Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
title_fullStr Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
title_full_unstemmed Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
title_short Human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
title_sort human testis-expressed sequence 101 is limitedly distributed in germinal epithelium of testis and disappears in seminoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4197282/
https://www.ncbi.nlm.nih.gov/pubmed/25418358
http://dx.doi.org/10.1186/0717-6287-47-52
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