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OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice

KEY MESSAGE: An ABC transporter gene ( OsABCG15 ) was proven to be involved in pollen development in rice. The corresponding protein was localized on the plasma membrane using subcellular localization. ABSTRACT: Wax, cutin, and sporopollenin are important for normal development of the anther cuticle...

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Autores principales: Wu, Lina, Guan, Yusheng, Wu, Zigang, Yang, Kun, Lv, Jun, Converse, Richard, Huang, Yuanxin, Mao, Jinxiong, Zhao, Yong, Wang, Zhongwei, Min, Hengqi, Kan, Dongyang, Zhang, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4197380/
https://www.ncbi.nlm.nih.gov/pubmed/25138437
http://dx.doi.org/10.1007/s00299-014-1666-8
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author Wu, Lina
Guan, Yusheng
Wu, Zigang
Yang, Kun
Lv, Jun
Converse, Richard
Huang, Yuanxin
Mao, Jinxiong
Zhao, Yong
Wang, Zhongwei
Min, Hengqi
Kan, Dongyang
Zhang, Yi
author_facet Wu, Lina
Guan, Yusheng
Wu, Zigang
Yang, Kun
Lv, Jun
Converse, Richard
Huang, Yuanxin
Mao, Jinxiong
Zhao, Yong
Wang, Zhongwei
Min, Hengqi
Kan, Dongyang
Zhang, Yi
author_sort Wu, Lina
collection PubMed
description KEY MESSAGE: An ABC transporter gene ( OsABCG15 ) was proven to be involved in pollen development in rice. The corresponding protein was localized on the plasma membrane using subcellular localization. ABSTRACT: Wax, cutin, and sporopollenin are important for normal development of the anther cuticle and pollen exine, respectively. Their lipid soluble precursors, which are produced in the tapetum, are then secreted and transferred to the anther and microspore surface for polymerization. However, little is known about the mechanisms underlying the transport of these precursors. Here, we identified and characterized a member of the G subfamily of ATP-binding cassette (ABC) transporters, OsABCG15, which is required for the secretion of these lipid-soluble precursors in rice. Using map-based cloning, we found a spontaneous A-to-C transition in the fourth exon of OsABCG15 that caused an amino acid substitution of Thr-to-Pro in the predicted ATP-binding domain of the protein sequence. This osabcg15 mutant failed to produce any viable pollen and was completely male sterile. Histological analysis indicated that osabcg15 exhibited an undeveloped anther cuticle, enlarged middle layer, abnormal Ubisch body development, tapetum degeneration with a falling apart style, and collapsed pollen grains without detectable exine. OsABCG15 was expressed preferentially in the tapetum, and the fused GFP-OsABCG15 protein was localized to the plasma membrane. Our results suggested that OsABCG15 played an essential role in the formation of the rice anther cuticle and pollen exine. This role may include the secretion of the lipid precursors from the tapetum to facilitate the transfer of precursors to the surface of the anther epidermis as well as to microspores. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00299-014-1666-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-41973802014-10-20 OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice Wu, Lina Guan, Yusheng Wu, Zigang Yang, Kun Lv, Jun Converse, Richard Huang, Yuanxin Mao, Jinxiong Zhao, Yong Wang, Zhongwei Min, Hengqi Kan, Dongyang Zhang, Yi Plant Cell Rep Original Paper KEY MESSAGE: An ABC transporter gene ( OsABCG15 ) was proven to be involved in pollen development in rice. The corresponding protein was localized on the plasma membrane using subcellular localization. ABSTRACT: Wax, cutin, and sporopollenin are important for normal development of the anther cuticle and pollen exine, respectively. Their lipid soluble precursors, which are produced in the tapetum, are then secreted and transferred to the anther and microspore surface for polymerization. However, little is known about the mechanisms underlying the transport of these precursors. Here, we identified and characterized a member of the G subfamily of ATP-binding cassette (ABC) transporters, OsABCG15, which is required for the secretion of these lipid-soluble precursors in rice. Using map-based cloning, we found a spontaneous A-to-C transition in the fourth exon of OsABCG15 that caused an amino acid substitution of Thr-to-Pro in the predicted ATP-binding domain of the protein sequence. This osabcg15 mutant failed to produce any viable pollen and was completely male sterile. Histological analysis indicated that osabcg15 exhibited an undeveloped anther cuticle, enlarged middle layer, abnormal Ubisch body development, tapetum degeneration with a falling apart style, and collapsed pollen grains without detectable exine. OsABCG15 was expressed preferentially in the tapetum, and the fused GFP-OsABCG15 protein was localized to the plasma membrane. Our results suggested that OsABCG15 played an essential role in the formation of the rice anther cuticle and pollen exine. This role may include the secretion of the lipid precursors from the tapetum to facilitate the transfer of precursors to the surface of the anther epidermis as well as to microspores. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00299-014-1666-8) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2014-08-20 2014 /pmc/articles/PMC4197380/ /pubmed/25138437 http://dx.doi.org/10.1007/s00299-014-1666-8 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Wu, Lina
Guan, Yusheng
Wu, Zigang
Yang, Kun
Lv, Jun
Converse, Richard
Huang, Yuanxin
Mao, Jinxiong
Zhao, Yong
Wang, Zhongwei
Min, Hengqi
Kan, Dongyang
Zhang, Yi
OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice
title OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice
title_full OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice
title_fullStr OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice
title_full_unstemmed OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice
title_short OsABCG15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice
title_sort osabcg15 encodes a membrane protein that plays an important role in anther cuticle and pollen exine formation in rice
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4197380/
https://www.ncbi.nlm.nih.gov/pubmed/25138437
http://dx.doi.org/10.1007/s00299-014-1666-8
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