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The Fusarium graminearum Genome Reveals More Secondary Metabolite Gene Clusters and Hints of Horizontal Gene Transfer

Fungal secondary metabolite biosynthesis genes are of major interest due to the pharmacological properties of their products (like mycotoxins and antibiotics). The genome of the plant pathogenic fungus Fusarium graminearum codes for a large number of candidate enzymes involved in secondary metabolit...

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Autores principales: Sieber, Christian M. K., Lee, Wanseon, Wong, Philip, Münsterkötter, Martin, Mewes, Hans-Werner, Schmeitzl, Clemens, Varga, Elisabeth, Berthiller, Franz, Adam, Gerhard, Güldener, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198257/
https://www.ncbi.nlm.nih.gov/pubmed/25333987
http://dx.doi.org/10.1371/journal.pone.0110311
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author Sieber, Christian M. K.
Lee, Wanseon
Wong, Philip
Münsterkötter, Martin
Mewes, Hans-Werner
Schmeitzl, Clemens
Varga, Elisabeth
Berthiller, Franz
Adam, Gerhard
Güldener, Ulrich
author_facet Sieber, Christian M. K.
Lee, Wanseon
Wong, Philip
Münsterkötter, Martin
Mewes, Hans-Werner
Schmeitzl, Clemens
Varga, Elisabeth
Berthiller, Franz
Adam, Gerhard
Güldener, Ulrich
author_sort Sieber, Christian M. K.
collection PubMed
description Fungal secondary metabolite biosynthesis genes are of major interest due to the pharmacological properties of their products (like mycotoxins and antibiotics). The genome of the plant pathogenic fungus Fusarium graminearum codes for a large number of candidate enzymes involved in secondary metabolite biosynthesis. However, the chemical nature of most enzymatic products of proteins encoded by putative secondary metabolism biosynthetic genes is largely unknown. Based on our analysis we present 67 gene clusters with significant enrichment of predicted secondary metabolism related enzymatic functions. 20 gene clusters with unknown metabolites exhibit strong gene expression correlation in planta and presumably play a role in virulence. Furthermore, the identification of conserved and over-represented putative transcription factor binding sites serves as additional evidence for cluster co-regulation. Orthologous cluster search provided insight into the evolution of secondary metabolism clusters. Some clusters are characteristic for the Fusarium phylum while others show evidence of horizontal gene transfer as orthologs can be found in representatives of the Botrytis or Cochliobolus lineage. The presented candidate clusters provide valuable targets for experimental examination.
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spelling pubmed-41982572014-10-21 The Fusarium graminearum Genome Reveals More Secondary Metabolite Gene Clusters and Hints of Horizontal Gene Transfer Sieber, Christian M. K. Lee, Wanseon Wong, Philip Münsterkötter, Martin Mewes, Hans-Werner Schmeitzl, Clemens Varga, Elisabeth Berthiller, Franz Adam, Gerhard Güldener, Ulrich PLoS One Research Article Fungal secondary metabolite biosynthesis genes are of major interest due to the pharmacological properties of their products (like mycotoxins and antibiotics). The genome of the plant pathogenic fungus Fusarium graminearum codes for a large number of candidate enzymes involved in secondary metabolite biosynthesis. However, the chemical nature of most enzymatic products of proteins encoded by putative secondary metabolism biosynthetic genes is largely unknown. Based on our analysis we present 67 gene clusters with significant enrichment of predicted secondary metabolism related enzymatic functions. 20 gene clusters with unknown metabolites exhibit strong gene expression correlation in planta and presumably play a role in virulence. Furthermore, the identification of conserved and over-represented putative transcription factor binding sites serves as additional evidence for cluster co-regulation. Orthologous cluster search provided insight into the evolution of secondary metabolism clusters. Some clusters are characteristic for the Fusarium phylum while others show evidence of horizontal gene transfer as orthologs can be found in representatives of the Botrytis or Cochliobolus lineage. The presented candidate clusters provide valuable targets for experimental examination. Public Library of Science 2014-10-15 /pmc/articles/PMC4198257/ /pubmed/25333987 http://dx.doi.org/10.1371/journal.pone.0110311 Text en © 2014 Sieber et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sieber, Christian M. K.
Lee, Wanseon
Wong, Philip
Münsterkötter, Martin
Mewes, Hans-Werner
Schmeitzl, Clemens
Varga, Elisabeth
Berthiller, Franz
Adam, Gerhard
Güldener, Ulrich
The Fusarium graminearum Genome Reveals More Secondary Metabolite Gene Clusters and Hints of Horizontal Gene Transfer
title The Fusarium graminearum Genome Reveals More Secondary Metabolite Gene Clusters and Hints of Horizontal Gene Transfer
title_full The Fusarium graminearum Genome Reveals More Secondary Metabolite Gene Clusters and Hints of Horizontal Gene Transfer
title_fullStr The Fusarium graminearum Genome Reveals More Secondary Metabolite Gene Clusters and Hints of Horizontal Gene Transfer
title_full_unstemmed The Fusarium graminearum Genome Reveals More Secondary Metabolite Gene Clusters and Hints of Horizontal Gene Transfer
title_short The Fusarium graminearum Genome Reveals More Secondary Metabolite Gene Clusters and Hints of Horizontal Gene Transfer
title_sort fusarium graminearum genome reveals more secondary metabolite gene clusters and hints of horizontal gene transfer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198257/
https://www.ncbi.nlm.nih.gov/pubmed/25333987
http://dx.doi.org/10.1371/journal.pone.0110311
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