Cargando…

Seroprevalence of human T lymphotropic virus antibodies among healthy blood donors at a tertiary centre in Lagos, Nigeria

INTRODUCTION: Transmission of human T-lymphotropic viruses (HTLV) occurs from mother to child, by sexual contact and blood transfusion. Presently, in most centres in Nigeria, there is no routine pre-transfusion screening for HTLV. The study aims to determine the prevalence of HTLV-1 and HTLV-2 among...

Descripción completa

Detalles Bibliográficos
Autores principales: Durojaiye, Idris, Akinbami, Akinsegun, Dosunmu, Adedoyin, Ajibola, Sarah, Adediran, Adewumi, Uche, Ebele, Oshinaike, Olajumoke, Odesanya, Majeed, Dada, Akinola, Okunoye, Olaitan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The African Field Epidemiology Network 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198262/
https://www.ncbi.nlm.nih.gov/pubmed/25328597
http://dx.doi.org/10.11604/pamj.2014.17.301.4075
_version_ 1782339718685392896
author Durojaiye, Idris
Akinbami, Akinsegun
Dosunmu, Adedoyin
Ajibola, Sarah
Adediran, Adewumi
Uche, Ebele
Oshinaike, Olajumoke
Odesanya, Majeed
Dada, Akinola
Okunoye, Olaitan
author_facet Durojaiye, Idris
Akinbami, Akinsegun
Dosunmu, Adedoyin
Ajibola, Sarah
Adediran, Adewumi
Uche, Ebele
Oshinaike, Olajumoke
Odesanya, Majeed
Dada, Akinola
Okunoye, Olaitan
author_sort Durojaiye, Idris
collection PubMed
description INTRODUCTION: Transmission of human T-lymphotropic viruses (HTLV) occurs from mother to child, by sexual contact and blood transfusion. Presently, in most centres in Nigeria, there is no routine pre-transfusion screening for HTLV. The study aims to determine the prevalence of HTLV-1 and HTLV-2 among healthy blood donors at a tertiary centre in Lagos. METHODS: A cross-sectional study was carried out at the blood donor clinic of the Lagos State University Teaching Hospital (LASUTH), Ikeja. About 5mls of venous blood was collected from each subject into a sterile plain bottle after obtaining subject's consent. The serum separated and stored at -200C. Sera were assayed for HTLV by an enzyme-linked immunoassay (ELISA) for the determination of antibodies to HTLV 1 and HTLV -2. Western blot confirmatory testing was done on reactive samples. All donors were also screened for HIV, HBsAg and HCV by rapid kits. RESULTS: The seroprevalence of HTLV -1 by ELISA was 1.0% and 0.5% by Western Blot among blood donors. A total of 210 healthy blood donors were enrolled. Only 2 (1.0%) blood donors were repeatedly reactive with ELISA test. On confirmatory testing with Western Blot, 1 (0.5%) blood donor was positive for HTLV. All the healthy blood donors were negative for HIV, HbsAg and HCV. None of the 210 blood donors had been previously transfused; as such no association could be established between transfusion history and HTLV positivity among the blood donors. CONCLUSION: The seroprevalence of HTLV in this environment is low among healthy blood donors.
format Online
Article
Text
id pubmed-4198262
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher The African Field Epidemiology Network
record_format MEDLINE/PubMed
spelling pubmed-41982622014-10-17 Seroprevalence of human T lymphotropic virus antibodies among healthy blood donors at a tertiary centre in Lagos, Nigeria Durojaiye, Idris Akinbami, Akinsegun Dosunmu, Adedoyin Ajibola, Sarah Adediran, Adewumi Uche, Ebele Oshinaike, Olajumoke Odesanya, Majeed Dada, Akinola Okunoye, Olaitan Pan Afr Med J Research INTRODUCTION: Transmission of human T-lymphotropic viruses (HTLV) occurs from mother to child, by sexual contact and blood transfusion. Presently, in most centres in Nigeria, there is no routine pre-transfusion screening for HTLV. The study aims to determine the prevalence of HTLV-1 and HTLV-2 among healthy blood donors at a tertiary centre in Lagos. METHODS: A cross-sectional study was carried out at the blood donor clinic of the Lagos State University Teaching Hospital (LASUTH), Ikeja. About 5mls of venous blood was collected from each subject into a sterile plain bottle after obtaining subject's consent. The serum separated and stored at -200C. Sera were assayed for HTLV by an enzyme-linked immunoassay (ELISA) for the determination of antibodies to HTLV 1 and HTLV -2. Western blot confirmatory testing was done on reactive samples. All donors were also screened for HIV, HBsAg and HCV by rapid kits. RESULTS: The seroprevalence of HTLV -1 by ELISA was 1.0% and 0.5% by Western Blot among blood donors. A total of 210 healthy blood donors were enrolled. Only 2 (1.0%) blood donors were repeatedly reactive with ELISA test. On confirmatory testing with Western Blot, 1 (0.5%) blood donor was positive for HTLV. All the healthy blood donors were negative for HIV, HbsAg and HCV. None of the 210 blood donors had been previously transfused; as such no association could be established between transfusion history and HTLV positivity among the blood donors. CONCLUSION: The seroprevalence of HTLV in this environment is low among healthy blood donors. The African Field Epidemiology Network 2014-04-21 /pmc/articles/PMC4198262/ /pubmed/25328597 http://dx.doi.org/10.11604/pamj.2014.17.301.4075 Text en © Idris Durojaiye et al. http://creativecommons.org/licenses/by/2.0/ The Pan African Medical Journal - ISSN 1937-8688. This is an Open Access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Durojaiye, Idris
Akinbami, Akinsegun
Dosunmu, Adedoyin
Ajibola, Sarah
Adediran, Adewumi
Uche, Ebele
Oshinaike, Olajumoke
Odesanya, Majeed
Dada, Akinola
Okunoye, Olaitan
Seroprevalence of human T lymphotropic virus antibodies among healthy blood donors at a tertiary centre in Lagos, Nigeria
title Seroprevalence of human T lymphotropic virus antibodies among healthy blood donors at a tertiary centre in Lagos, Nigeria
title_full Seroprevalence of human T lymphotropic virus antibodies among healthy blood donors at a tertiary centre in Lagos, Nigeria
title_fullStr Seroprevalence of human T lymphotropic virus antibodies among healthy blood donors at a tertiary centre in Lagos, Nigeria
title_full_unstemmed Seroprevalence of human T lymphotropic virus antibodies among healthy blood donors at a tertiary centre in Lagos, Nigeria
title_short Seroprevalence of human T lymphotropic virus antibodies among healthy blood donors at a tertiary centre in Lagos, Nigeria
title_sort seroprevalence of human t lymphotropic virus antibodies among healthy blood donors at a tertiary centre in lagos, nigeria
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198262/
https://www.ncbi.nlm.nih.gov/pubmed/25328597
http://dx.doi.org/10.11604/pamj.2014.17.301.4075
work_keys_str_mv AT durojaiyeidris seroprevalenceofhumantlymphotropicvirusantibodiesamonghealthyblooddonorsatatertiarycentreinlagosnigeria
AT akinbamiakinsegun seroprevalenceofhumantlymphotropicvirusantibodiesamonghealthyblooddonorsatatertiarycentreinlagosnigeria
AT dosunmuadedoyin seroprevalenceofhumantlymphotropicvirusantibodiesamonghealthyblooddonorsatatertiarycentreinlagosnigeria
AT ajibolasarah seroprevalenceofhumantlymphotropicvirusantibodiesamonghealthyblooddonorsatatertiarycentreinlagosnigeria
AT adediranadewumi seroprevalenceofhumantlymphotropicvirusantibodiesamonghealthyblooddonorsatatertiarycentreinlagosnigeria
AT ucheebele seroprevalenceofhumantlymphotropicvirusantibodiesamonghealthyblooddonorsatatertiarycentreinlagosnigeria
AT oshinaikeolajumoke seroprevalenceofhumantlymphotropicvirusantibodiesamonghealthyblooddonorsatatertiarycentreinlagosnigeria
AT odesanyamajeed seroprevalenceofhumantlymphotropicvirusantibodiesamonghealthyblooddonorsatatertiarycentreinlagosnigeria
AT dadaakinola seroprevalenceofhumantlymphotropicvirusantibodiesamonghealthyblooddonorsatatertiarycentreinlagosnigeria
AT okunoyeolaitan seroprevalenceofhumantlymphotropicvirusantibodiesamonghealthyblooddonorsatatertiarycentreinlagosnigeria