Cargando…

Genome-wide association study for somatic cell score in Valdostana Red Pied cattle breed using pooled DNA

BACKGROUND: Mastitis is a major disease of dairy cattle occurring in response to environmental exposure to infective agents with a great economic impact on dairy industry. Somatic cell count (SCC) and its log transformation in somatic cell score (SCS) are traits that have been used as indirect measu...

Descripción completa

Detalles Bibliográficos
Autores principales: Strillacci, Maria G, Frigo, Erika, Schiavini, Fausta, Samoré, Antonia B, Canavesi, Fabiola, Vevey, Mario, Cozzi, Maria C, Soller, Morris, Lipkin, Ehud, Bagnato, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198737/
https://www.ncbi.nlm.nih.gov/pubmed/25288516
http://dx.doi.org/10.1186/s12863-014-0106-7
_version_ 1782339775765676032
author Strillacci, Maria G
Frigo, Erika
Schiavini, Fausta
Samoré, Antonia B
Canavesi, Fabiola
Vevey, Mario
Cozzi, Maria C
Soller, Morris
Lipkin, Ehud
Bagnato, Alessandro
author_facet Strillacci, Maria G
Frigo, Erika
Schiavini, Fausta
Samoré, Antonia B
Canavesi, Fabiola
Vevey, Mario
Cozzi, Maria C
Soller, Morris
Lipkin, Ehud
Bagnato, Alessandro
author_sort Strillacci, Maria G
collection PubMed
description BACKGROUND: Mastitis is a major disease of dairy cattle occurring in response to environmental exposure to infective agents with a great economic impact on dairy industry. Somatic cell count (SCC) and its log transformation in somatic cell score (SCS) are traits that have been used as indirect measures of resistance to mastitis for decades in selective breeding. A selective DNA pooling (SDP) approach was applied to identify Quantitative Trait Loci (QTL) for SCS in Valdostana Red Pied cattle using the Illumina Bovine HD BeadChip. RESULTS: A total of 171 SNPs reached the genome-wide significance for association with SCS. Fifty-two SNPs were annotated within genes, some of those involved in the immune response to mastitis. On BTAs 1, 2, 3, 4, 9, 13, 15, 17, 21 and 22 the largest number of markers in association to the trait was found. These regions identified novel genomic regions related to mastitis (1-Mb SNP windows) and confirmed those already mapped. The largest number of significant SNPs exceeding the threshold for genome-wide significant signal was found on BTA 15, located at 50.43-51.63 Mb. CONCLUSIONS: The genomic regions identified in this study contribute to a better understanding of the genetic control of the mastitis immune response in cattle and may allow the inclusion of more detailed QTL information in selection programs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12863-014-0106-7) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4198737
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-41987372014-10-23 Genome-wide association study for somatic cell score in Valdostana Red Pied cattle breed using pooled DNA Strillacci, Maria G Frigo, Erika Schiavini, Fausta Samoré, Antonia B Canavesi, Fabiola Vevey, Mario Cozzi, Maria C Soller, Morris Lipkin, Ehud Bagnato, Alessandro BMC Genet Research Article BACKGROUND: Mastitis is a major disease of dairy cattle occurring in response to environmental exposure to infective agents with a great economic impact on dairy industry. Somatic cell count (SCC) and its log transformation in somatic cell score (SCS) are traits that have been used as indirect measures of resistance to mastitis for decades in selective breeding. A selective DNA pooling (SDP) approach was applied to identify Quantitative Trait Loci (QTL) for SCS in Valdostana Red Pied cattle using the Illumina Bovine HD BeadChip. RESULTS: A total of 171 SNPs reached the genome-wide significance for association with SCS. Fifty-two SNPs were annotated within genes, some of those involved in the immune response to mastitis. On BTAs 1, 2, 3, 4, 9, 13, 15, 17, 21 and 22 the largest number of markers in association to the trait was found. These regions identified novel genomic regions related to mastitis (1-Mb SNP windows) and confirmed those already mapped. The largest number of significant SNPs exceeding the threshold for genome-wide significant signal was found on BTA 15, located at 50.43-51.63 Mb. CONCLUSIONS: The genomic regions identified in this study contribute to a better understanding of the genetic control of the mastitis immune response in cattle and may allow the inclusion of more detailed QTL information in selection programs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12863-014-0106-7) contains supplementary material, which is available to authorized users. BioMed Central 2014-10-06 /pmc/articles/PMC4198737/ /pubmed/25288516 http://dx.doi.org/10.1186/s12863-014-0106-7 Text en © Strillacci et al.; licensee BioMed Central Ltd. 2014 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Strillacci, Maria G
Frigo, Erika
Schiavini, Fausta
Samoré, Antonia B
Canavesi, Fabiola
Vevey, Mario
Cozzi, Maria C
Soller, Morris
Lipkin, Ehud
Bagnato, Alessandro
Genome-wide association study for somatic cell score in Valdostana Red Pied cattle breed using pooled DNA
title Genome-wide association study for somatic cell score in Valdostana Red Pied cattle breed using pooled DNA
title_full Genome-wide association study for somatic cell score in Valdostana Red Pied cattle breed using pooled DNA
title_fullStr Genome-wide association study for somatic cell score in Valdostana Red Pied cattle breed using pooled DNA
title_full_unstemmed Genome-wide association study for somatic cell score in Valdostana Red Pied cattle breed using pooled DNA
title_short Genome-wide association study for somatic cell score in Valdostana Red Pied cattle breed using pooled DNA
title_sort genome-wide association study for somatic cell score in valdostana red pied cattle breed using pooled dna
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198737/
https://www.ncbi.nlm.nih.gov/pubmed/25288516
http://dx.doi.org/10.1186/s12863-014-0106-7
work_keys_str_mv AT strillaccimariag genomewideassociationstudyforsomaticcellscoreinvaldostanaredpiedcattlebreedusingpooleddna
AT frigoerika genomewideassociationstudyforsomaticcellscoreinvaldostanaredpiedcattlebreedusingpooleddna
AT schiavinifausta genomewideassociationstudyforsomaticcellscoreinvaldostanaredpiedcattlebreedusingpooleddna
AT samoreantoniab genomewideassociationstudyforsomaticcellscoreinvaldostanaredpiedcattlebreedusingpooleddna
AT canavesifabiola genomewideassociationstudyforsomaticcellscoreinvaldostanaredpiedcattlebreedusingpooleddna
AT veveymario genomewideassociationstudyforsomaticcellscoreinvaldostanaredpiedcattlebreedusingpooleddna
AT cozzimariac genomewideassociationstudyforsomaticcellscoreinvaldostanaredpiedcattlebreedusingpooleddna
AT sollermorris genomewideassociationstudyforsomaticcellscoreinvaldostanaredpiedcattlebreedusingpooleddna
AT lipkinehud genomewideassociationstudyforsomaticcellscoreinvaldostanaredpiedcattlebreedusingpooleddna
AT bagnatoalessandro genomewideassociationstudyforsomaticcellscoreinvaldostanaredpiedcattlebreedusingpooleddna