DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks

The modification of DNA by phosphorothioate (PT) occurs when the non-bridging oxygen in the sugar-phosphate backbone of DNA is replaced with sulfur. This DNA backbone modification was recently discovered and is governed by the dndABCDE genes in a diverse group of bacteria and archaea. However, the b...

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Autores principales: Gan, Rui, Wu, Xiaolin, He, Wei, Liu, Zhenhua, Wu, Shuangju, Chen, Chao, Chen, Si, Xiang, Qianrong, Deng, Zixin, Liang, Dequan, Chen, Shi, Wang, Lianrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198939/
https://www.ncbi.nlm.nih.gov/pubmed/25319634
http://dx.doi.org/10.1038/srep06642
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author Gan, Rui
Wu, Xiaolin
He, Wei
Liu, Zhenhua
Wu, Shuangju
Chen, Chao
Chen, Si
Xiang, Qianrong
Deng, Zixin
Liang, Dequan
Chen, Shi
Wang, Lianrong
author_facet Gan, Rui
Wu, Xiaolin
He, Wei
Liu, Zhenhua
Wu, Shuangju
Chen, Chao
Chen, Si
Xiang, Qianrong
Deng, Zixin
Liang, Dequan
Chen, Shi
Wang, Lianrong
author_sort Gan, Rui
collection PubMed
description The modification of DNA by phosphorothioate (PT) occurs when the non-bridging oxygen in the sugar-phosphate backbone of DNA is replaced with sulfur. This DNA backbone modification was recently discovered and is governed by the dndABCDE genes in a diverse group of bacteria and archaea. However, the biological function of DNA PT modifications is poorly understood. In this study, we employed the RNA-seq analysis to characterize the global transcriptional changes in response to PT modifications. Our results show that DNA without PT protection is susceptible to DNA damage caused by the dndFGHI gene products. The DNA double-stranded breaks then trigger the SOS response, cell filamentation and prophage induction. Heterologous expression of dndBCDE conferring DNA PT modifications at G(PS)A and G(PS)T prevented the damage in Salmonella enterica. Our data provide insights into the physiological role of the DNA PT system.
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spelling pubmed-41989392014-10-21 DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks Gan, Rui Wu, Xiaolin He, Wei Liu, Zhenhua Wu, Shuangju Chen, Chao Chen, Si Xiang, Qianrong Deng, Zixin Liang, Dequan Chen, Shi Wang, Lianrong Sci Rep Article The modification of DNA by phosphorothioate (PT) occurs when the non-bridging oxygen in the sugar-phosphate backbone of DNA is replaced with sulfur. This DNA backbone modification was recently discovered and is governed by the dndABCDE genes in a diverse group of bacteria and archaea. However, the biological function of DNA PT modifications is poorly understood. In this study, we employed the RNA-seq analysis to characterize the global transcriptional changes in response to PT modifications. Our results show that DNA without PT protection is susceptible to DNA damage caused by the dndFGHI gene products. The DNA double-stranded breaks then trigger the SOS response, cell filamentation and prophage induction. Heterologous expression of dndBCDE conferring DNA PT modifications at G(PS)A and G(PS)T prevented the damage in Salmonella enterica. Our data provide insights into the physiological role of the DNA PT system. Nature Publishing Group 2014-10-16 /pmc/articles/PMC4198939/ /pubmed/25319634 http://dx.doi.org/10.1038/srep06642 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Gan, Rui
Wu, Xiaolin
He, Wei
Liu, Zhenhua
Wu, Shuangju
Chen, Chao
Chen, Si
Xiang, Qianrong
Deng, Zixin
Liang, Dequan
Chen, Shi
Wang, Lianrong
DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks
title DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks
title_full DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks
title_fullStr DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks
title_full_unstemmed DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks
title_short DNA phosphorothioate modifications influence the global transcriptional response and protect DNA from double-stranded breaks
title_sort dna phosphorothioate modifications influence the global transcriptional response and protect dna from double-stranded breaks
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4198939/
https://www.ncbi.nlm.nih.gov/pubmed/25319634
http://dx.doi.org/10.1038/srep06642
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