Cargando…

Salt-Induced Stabilization of EIN3/EIL1 Confers Salinity Tolerance by Deterring ROS Accumulation in Arabidopsis

Ethylene has been regarded as a stress hormone to regulate myriad stress responses. Salinity stress is one of the most serious abiotic stresses limiting plant growth and development. But how ethylene signaling is involved in plant response to salt stress is poorly understood. Here we showed that Ara...

Descripción completa

Detalles Bibliográficos
Autores principales: Peng, Jinying, Li, Zhonghai, Wen, Xing, Li, Wenyang, Shi, Hui, Yang, Longshu, Zhu, Huaiqiu, Guo, Hongwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199496/
https://www.ncbi.nlm.nih.gov/pubmed/25330213
http://dx.doi.org/10.1371/journal.pgen.1004664
_version_ 1782339915969724416
author Peng, Jinying
Li, Zhonghai
Wen, Xing
Li, Wenyang
Shi, Hui
Yang, Longshu
Zhu, Huaiqiu
Guo, Hongwei
author_facet Peng, Jinying
Li, Zhonghai
Wen, Xing
Li, Wenyang
Shi, Hui
Yang, Longshu
Zhu, Huaiqiu
Guo, Hongwei
author_sort Peng, Jinying
collection PubMed
description Ethylene has been regarded as a stress hormone to regulate myriad stress responses. Salinity stress is one of the most serious abiotic stresses limiting plant growth and development. But how ethylene signaling is involved in plant response to salt stress is poorly understood. Here we showed that Arabidopsis plants pretreated with ethylene exhibited enhanced tolerance to salt stress. Gain- and loss-of-function studies demonstrated that EIN3 (ETHYLENE INSENSITIVE 3) and EIL1 (EIN3-LIKE 1), two ethylene-activated transcription factors, are necessary and sufficient for the enhanced salt tolerance. High salinity induced the accumulation of EIN3/EIL1 proteins by promoting the proteasomal degradation of two EIN3/EIL1-targeting F-box proteins, EBF1 and EBF2, in an EIN2-independent manner. Whole-genome transcriptome analysis identified a list of SIED (Salt-Induced and EIN3/EIL1-Dependent) genes that participate in salt stress responses, including several genes encoding reactive oxygen species (ROS) scavengers. We performed a genetic screen for ein3 eil1-like salt-hypersensitive mutants and identified 5 EIN3 direct target genes including a previously unknown gene, SIED1 (At5g22270), which encodes a 93-amino acid polypeptide involved in ROS dismissal. We also found that activation of EIN3 increased peroxidase (POD) activity through the direct transcriptional regulation of PODs expression. Accordingly, ethylene pretreatment or EIN3 activation was able to preclude excess ROS accumulation and increased tolerance to salt stress. Taken together, our study provides new insights into the molecular action of ethylene signaling to enhance plant salt tolerance, and elucidates the transcriptional network of EIN3 in salt stress response.
format Online
Article
Text
id pubmed-4199496
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-41994962014-10-21 Salt-Induced Stabilization of EIN3/EIL1 Confers Salinity Tolerance by Deterring ROS Accumulation in Arabidopsis Peng, Jinying Li, Zhonghai Wen, Xing Li, Wenyang Shi, Hui Yang, Longshu Zhu, Huaiqiu Guo, Hongwei PLoS Genet Research Article Ethylene has been regarded as a stress hormone to regulate myriad stress responses. Salinity stress is one of the most serious abiotic stresses limiting plant growth and development. But how ethylene signaling is involved in plant response to salt stress is poorly understood. Here we showed that Arabidopsis plants pretreated with ethylene exhibited enhanced tolerance to salt stress. Gain- and loss-of-function studies demonstrated that EIN3 (ETHYLENE INSENSITIVE 3) and EIL1 (EIN3-LIKE 1), two ethylene-activated transcription factors, are necessary and sufficient for the enhanced salt tolerance. High salinity induced the accumulation of EIN3/EIL1 proteins by promoting the proteasomal degradation of two EIN3/EIL1-targeting F-box proteins, EBF1 and EBF2, in an EIN2-independent manner. Whole-genome transcriptome analysis identified a list of SIED (Salt-Induced and EIN3/EIL1-Dependent) genes that participate in salt stress responses, including several genes encoding reactive oxygen species (ROS) scavengers. We performed a genetic screen for ein3 eil1-like salt-hypersensitive mutants and identified 5 EIN3 direct target genes including a previously unknown gene, SIED1 (At5g22270), which encodes a 93-amino acid polypeptide involved in ROS dismissal. We also found that activation of EIN3 increased peroxidase (POD) activity through the direct transcriptional regulation of PODs expression. Accordingly, ethylene pretreatment or EIN3 activation was able to preclude excess ROS accumulation and increased tolerance to salt stress. Taken together, our study provides new insights into the molecular action of ethylene signaling to enhance plant salt tolerance, and elucidates the transcriptional network of EIN3 in salt stress response. Public Library of Science 2014-10-16 /pmc/articles/PMC4199496/ /pubmed/25330213 http://dx.doi.org/10.1371/journal.pgen.1004664 Text en © 2014 Peng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Peng, Jinying
Li, Zhonghai
Wen, Xing
Li, Wenyang
Shi, Hui
Yang, Longshu
Zhu, Huaiqiu
Guo, Hongwei
Salt-Induced Stabilization of EIN3/EIL1 Confers Salinity Tolerance by Deterring ROS Accumulation in Arabidopsis
title Salt-Induced Stabilization of EIN3/EIL1 Confers Salinity Tolerance by Deterring ROS Accumulation in Arabidopsis
title_full Salt-Induced Stabilization of EIN3/EIL1 Confers Salinity Tolerance by Deterring ROS Accumulation in Arabidopsis
title_fullStr Salt-Induced Stabilization of EIN3/EIL1 Confers Salinity Tolerance by Deterring ROS Accumulation in Arabidopsis
title_full_unstemmed Salt-Induced Stabilization of EIN3/EIL1 Confers Salinity Tolerance by Deterring ROS Accumulation in Arabidopsis
title_short Salt-Induced Stabilization of EIN3/EIL1 Confers Salinity Tolerance by Deterring ROS Accumulation in Arabidopsis
title_sort salt-induced stabilization of ein3/eil1 confers salinity tolerance by deterring ros accumulation in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199496/
https://www.ncbi.nlm.nih.gov/pubmed/25330213
http://dx.doi.org/10.1371/journal.pgen.1004664
work_keys_str_mv AT pengjinying saltinducedstabilizationofein3eil1conferssalinitytolerancebydeterringrosaccumulationinarabidopsis
AT lizhonghai saltinducedstabilizationofein3eil1conferssalinitytolerancebydeterringrosaccumulationinarabidopsis
AT wenxing saltinducedstabilizationofein3eil1conferssalinitytolerancebydeterringrosaccumulationinarabidopsis
AT liwenyang saltinducedstabilizationofein3eil1conferssalinitytolerancebydeterringrosaccumulationinarabidopsis
AT shihui saltinducedstabilizationofein3eil1conferssalinitytolerancebydeterringrosaccumulationinarabidopsis
AT yanglongshu saltinducedstabilizationofein3eil1conferssalinitytolerancebydeterringrosaccumulationinarabidopsis
AT zhuhuaiqiu saltinducedstabilizationofein3eil1conferssalinitytolerancebydeterringrosaccumulationinarabidopsis
AT guohongwei saltinducedstabilizationofein3eil1conferssalinitytolerancebydeterringrosaccumulationinarabidopsis