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Ethylene-Induced Inhibition of Root Growth Requires Abscisic Acid Function in Rice (Oryza sativa L.) Seedlings

Ethylene and abscisic acid (ABA) have a complicated interplay in many developmental processes. Their interaction in rice is largely unclear. Here, we characterized a rice ethylene-response mutant mhz4, which exhibited reduced ethylene-response in roots but enhanced ethylene-response in coleoptiles o...

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Autores principales: Ma, Biao, Yin, Cui-Cui, He, Si-Jie, Lu, Xiang, Zhang, Wan-Ke, Lu, Tie-Gang, Chen, Shou-Yi, Zhang, Jin-Song
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199509/
https://www.ncbi.nlm.nih.gov/pubmed/25330236
http://dx.doi.org/10.1371/journal.pgen.1004701
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author Ma, Biao
Yin, Cui-Cui
He, Si-Jie
Lu, Xiang
Zhang, Wan-Ke
Lu, Tie-Gang
Chen, Shou-Yi
Zhang, Jin-Song
author_facet Ma, Biao
Yin, Cui-Cui
He, Si-Jie
Lu, Xiang
Zhang, Wan-Ke
Lu, Tie-Gang
Chen, Shou-Yi
Zhang, Jin-Song
author_sort Ma, Biao
collection PubMed
description Ethylene and abscisic acid (ABA) have a complicated interplay in many developmental processes. Their interaction in rice is largely unclear. Here, we characterized a rice ethylene-response mutant mhz4, which exhibited reduced ethylene-response in roots but enhanced ethylene-response in coleoptiles of etiolated seedlings. MHZ4 was identified through map-based cloning and encoded a chloroplast-localized membrane protein homologous to Arabidopsis thaliana (Arabidopsis) ABA4, which is responsible for a branch of ABA biosynthesis. MHZ4 mutation reduced ABA level, but promoted ethylene production. Ethylene induced MHZ4 expression and promoted ABA accumulation in roots. MHZ4 overexpression resulted in enhanced and reduced ethylene response in roots and coleoptiles, respectively. In root, MHZ4-dependent ABA pathway acts at or downstream of ethylene receptors and positively regulates root ethylene response. This ethylene-ABA interaction mode is different from that reported in Arabidopsis, where ethylene-mediated root inhibition is independent of ABA function. In coleoptile, MHZ4-dependent ABA pathway acts at or upstream of OsEIN2 to negatively regulate coleoptile ethylene response, possibly by affecting OsEIN2 expression. At mature stage, mhz4 mutation affects branching and adventitious root formation on stem nodes of higher positions, as well as yield-related traits. Together, our findings reveal a novel mode of interplay between ethylene and ABA in control of rice growth and development.
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spelling pubmed-41995092014-10-21 Ethylene-Induced Inhibition of Root Growth Requires Abscisic Acid Function in Rice (Oryza sativa L.) Seedlings Ma, Biao Yin, Cui-Cui He, Si-Jie Lu, Xiang Zhang, Wan-Ke Lu, Tie-Gang Chen, Shou-Yi Zhang, Jin-Song PLoS Genet Research Article Ethylene and abscisic acid (ABA) have a complicated interplay in many developmental processes. Their interaction in rice is largely unclear. Here, we characterized a rice ethylene-response mutant mhz4, which exhibited reduced ethylene-response in roots but enhanced ethylene-response in coleoptiles of etiolated seedlings. MHZ4 was identified through map-based cloning and encoded a chloroplast-localized membrane protein homologous to Arabidopsis thaliana (Arabidopsis) ABA4, which is responsible for a branch of ABA biosynthesis. MHZ4 mutation reduced ABA level, but promoted ethylene production. Ethylene induced MHZ4 expression and promoted ABA accumulation in roots. MHZ4 overexpression resulted in enhanced and reduced ethylene response in roots and coleoptiles, respectively. In root, MHZ4-dependent ABA pathway acts at or downstream of ethylene receptors and positively regulates root ethylene response. This ethylene-ABA interaction mode is different from that reported in Arabidopsis, where ethylene-mediated root inhibition is independent of ABA function. In coleoptile, MHZ4-dependent ABA pathway acts at or upstream of OsEIN2 to negatively regulate coleoptile ethylene response, possibly by affecting OsEIN2 expression. At mature stage, mhz4 mutation affects branching and adventitious root formation on stem nodes of higher positions, as well as yield-related traits. Together, our findings reveal a novel mode of interplay between ethylene and ABA in control of rice growth and development. Public Library of Science 2014-10-16 /pmc/articles/PMC4199509/ /pubmed/25330236 http://dx.doi.org/10.1371/journal.pgen.1004701 Text en © 2014 Ma et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ma, Biao
Yin, Cui-Cui
He, Si-Jie
Lu, Xiang
Zhang, Wan-Ke
Lu, Tie-Gang
Chen, Shou-Yi
Zhang, Jin-Song
Ethylene-Induced Inhibition of Root Growth Requires Abscisic Acid Function in Rice (Oryza sativa L.) Seedlings
title Ethylene-Induced Inhibition of Root Growth Requires Abscisic Acid Function in Rice (Oryza sativa L.) Seedlings
title_full Ethylene-Induced Inhibition of Root Growth Requires Abscisic Acid Function in Rice (Oryza sativa L.) Seedlings
title_fullStr Ethylene-Induced Inhibition of Root Growth Requires Abscisic Acid Function in Rice (Oryza sativa L.) Seedlings
title_full_unstemmed Ethylene-Induced Inhibition of Root Growth Requires Abscisic Acid Function in Rice (Oryza sativa L.) Seedlings
title_short Ethylene-Induced Inhibition of Root Growth Requires Abscisic Acid Function in Rice (Oryza sativa L.) Seedlings
title_sort ethylene-induced inhibition of root growth requires abscisic acid function in rice (oryza sativa l.) seedlings
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199509/
https://www.ncbi.nlm.nih.gov/pubmed/25330236
http://dx.doi.org/10.1371/journal.pgen.1004701
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