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Overexpression of MicroRNA-200c Predicts Poor Outcome in Patients with PR-Negative Breast Cancer
Micro-RNAs are small, noncoding RNAs that act as tumor suppressors or oncogenes. MiR-200c is a member of the miR-200 family; it is known to be dysregulated in invasive breast carcinoma. MiR-200c maintains the epithelial-mesenchymal transition and inhibits cell migration and invasion. Recent studies...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199599/ https://www.ncbi.nlm.nih.gov/pubmed/25329395 http://dx.doi.org/10.1371/journal.pone.0109508 |
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author | Tuomarila, Marie Luostari, Kaisa Soini, Ylermi Kataja, Vesa Kosma, Veli-Matti Mannermaa, Arto |
author_facet | Tuomarila, Marie Luostari, Kaisa Soini, Ylermi Kataja, Vesa Kosma, Veli-Matti Mannermaa, Arto |
author_sort | Tuomarila, Marie |
collection | PubMed |
description | Micro-RNAs are small, noncoding RNAs that act as tumor suppressors or oncogenes. MiR-200c is a member of the miR-200 family; it is known to be dysregulated in invasive breast carcinoma. MiR-200c maintains the epithelial-mesenchymal transition and inhibits cell migration and invasion. Recent studies showed that miR-200c regulated steroid hormone receptors, estrogen receptors (ER), and progesterone receptors (PR). The present study aimed to detect miR-200c in 172 invasive breast carcinoma cases selected from a prospective cohort enrolled in Kuopio, Eastern Finland, between 1990 and 1995. MiR-200c expression was determined with relative q-PCR, and results were compared to clinicopathological variables and patient outcome. We found that PR status combined with miR-200c expression was a significant marker of outcome. High miR-200c expression was associated with reduced survival in PR-negative cases (n = 68); low miR-200c expression indicated reduced survival in PR-positive cases (n = 86) (Cox regression: P = 0.002, OR = 3.433; and P = 0.004, OR = 4.176, respectively). In PR-negative cases, high miR-200c expression was associated with shortened relapse-free survival (Cox regression: P = 0.001, OR = 3.613); increased local/distant recurrence (Logistic regression: P = 0.006, OR = 3.965); and more frequent distant metastasis (Logistic regression: P = 0.015, OR = 3.390). We also found that high grade and low stage tumors were positively correlated with high miR-200c expression (Logistic regression for high grade tumors: P = 0.002, OR = 2.791 and for high stage tumors: P = 0.035, OR = 0.285). Our results indicated that miR-200c may play a role in invasive breast carcinoma. Furthermore, miR-200c combined with PR status provided a refined predictor of outcome. In future, a larger study is required to confirm our results. This data may provide a basis for new research target–progesterone receptor–regulated microRNAs in breast cancer. |
format | Online Article Text |
id | pubmed-4199599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41995992014-10-21 Overexpression of MicroRNA-200c Predicts Poor Outcome in Patients with PR-Negative Breast Cancer Tuomarila, Marie Luostari, Kaisa Soini, Ylermi Kataja, Vesa Kosma, Veli-Matti Mannermaa, Arto PLoS One Research Article Micro-RNAs are small, noncoding RNAs that act as tumor suppressors or oncogenes. MiR-200c is a member of the miR-200 family; it is known to be dysregulated in invasive breast carcinoma. MiR-200c maintains the epithelial-mesenchymal transition and inhibits cell migration and invasion. Recent studies showed that miR-200c regulated steroid hormone receptors, estrogen receptors (ER), and progesterone receptors (PR). The present study aimed to detect miR-200c in 172 invasive breast carcinoma cases selected from a prospective cohort enrolled in Kuopio, Eastern Finland, between 1990 and 1995. MiR-200c expression was determined with relative q-PCR, and results were compared to clinicopathological variables and patient outcome. We found that PR status combined with miR-200c expression was a significant marker of outcome. High miR-200c expression was associated with reduced survival in PR-negative cases (n = 68); low miR-200c expression indicated reduced survival in PR-positive cases (n = 86) (Cox regression: P = 0.002, OR = 3.433; and P = 0.004, OR = 4.176, respectively). In PR-negative cases, high miR-200c expression was associated with shortened relapse-free survival (Cox regression: P = 0.001, OR = 3.613); increased local/distant recurrence (Logistic regression: P = 0.006, OR = 3.965); and more frequent distant metastasis (Logistic regression: P = 0.015, OR = 3.390). We also found that high grade and low stage tumors were positively correlated with high miR-200c expression (Logistic regression for high grade tumors: P = 0.002, OR = 2.791 and for high stage tumors: P = 0.035, OR = 0.285). Our results indicated that miR-200c may play a role in invasive breast carcinoma. Furthermore, miR-200c combined with PR status provided a refined predictor of outcome. In future, a larger study is required to confirm our results. This data may provide a basis for new research target–progesterone receptor–regulated microRNAs in breast cancer. Public Library of Science 2014-10-16 /pmc/articles/PMC4199599/ /pubmed/25329395 http://dx.doi.org/10.1371/journal.pone.0109508 Text en © 2014 Tuomarila et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tuomarila, Marie Luostari, Kaisa Soini, Ylermi Kataja, Vesa Kosma, Veli-Matti Mannermaa, Arto Overexpression of MicroRNA-200c Predicts Poor Outcome in Patients with PR-Negative Breast Cancer |
title | Overexpression of MicroRNA-200c Predicts Poor Outcome in Patients with PR-Negative Breast Cancer |
title_full | Overexpression of MicroRNA-200c Predicts Poor Outcome in Patients with PR-Negative Breast Cancer |
title_fullStr | Overexpression of MicroRNA-200c Predicts Poor Outcome in Patients with PR-Negative Breast Cancer |
title_full_unstemmed | Overexpression of MicroRNA-200c Predicts Poor Outcome in Patients with PR-Negative Breast Cancer |
title_short | Overexpression of MicroRNA-200c Predicts Poor Outcome in Patients with PR-Negative Breast Cancer |
title_sort | overexpression of microrna-200c predicts poor outcome in patients with pr-negative breast cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199599/ https://www.ncbi.nlm.nih.gov/pubmed/25329395 http://dx.doi.org/10.1371/journal.pone.0109508 |
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