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Clinical, biomechanical and histological study on oophorectomy induced menopause
OBJECTIVE: To investigate the clinical implications as well as biomechanical and histological changes and in bone tissue induced by ovariectomy in 64 rats. METHODS: The rats were divided into two groups: bilateral oophorectomy or placebo, and subdivided into four subgroups, according to time postope...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Ortopedia e Traumatologia
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199643/ https://www.ncbi.nlm.nih.gov/pubmed/25328434 http://dx.doi.org/10.1590/1413-78522014220500420 |
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author | Bordinhon, Maristela Müller, Sérgio Swain Silva, Maeli Dal Pai |
author_facet | Bordinhon, Maristela Müller, Sérgio Swain Silva, Maeli Dal Pai |
author_sort | Bordinhon, Maristela |
collection | PubMed |
description | OBJECTIVE: To investigate the clinical implications as well as biomechanical and histological changes and in bone tissue induced by ovariectomy in 64 rats. METHODS: The rats were divided into two groups: bilateral oophorectomy or placebo, and subdivided into four subgroups, according to time postoperatively: three, six, nine and 12 months. The weight of the animals at the time of sacrifice was taken into consideration. The biomechanical study was performed on the right tibia, to the maximum load and stiffness coefficient. For the histological study we calculated the trabecular bone of the left tibia. Statistical analysis of body weight and mechanical properties was performed by variance analysis, complemented with Tukey's multiple comparison tests; and trabecular area, the non-parametric variance analysis. RESULTS: Ovariectomy-induced menopause caused an increase in body weight, reduction of diaphyseal bone resistance at six months of hormone deprivation, but this effect is equalized over time by aging; bone stiffness was smaller in the ovariectomized group and reduction of bone mass occurred. CONCLUSION: The removal of the ovaries produced systemic alterations, characterized by metabolic changes that caused weight gain and changes in bone tissue, associated with alteration of the mechanical profile and reduced bone mass. Level of Evidence I, Clinical Study. |
format | Online Article Text |
id | pubmed-4199643 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Ortopedia e Traumatologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-41996432014-10-17 Clinical, biomechanical and histological study on oophorectomy induced menopause Bordinhon, Maristela Müller, Sérgio Swain Silva, Maeli Dal Pai Acta Ortop Bras Original Article OBJECTIVE: To investigate the clinical implications as well as biomechanical and histological changes and in bone tissue induced by ovariectomy in 64 rats. METHODS: The rats were divided into two groups: bilateral oophorectomy or placebo, and subdivided into four subgroups, according to time postoperatively: three, six, nine and 12 months. The weight of the animals at the time of sacrifice was taken into consideration. The biomechanical study was performed on the right tibia, to the maximum load and stiffness coefficient. For the histological study we calculated the trabecular bone of the left tibia. Statistical analysis of body weight and mechanical properties was performed by variance analysis, complemented with Tukey's multiple comparison tests; and trabecular area, the non-parametric variance analysis. RESULTS: Ovariectomy-induced menopause caused an increase in body weight, reduction of diaphyseal bone resistance at six months of hormone deprivation, but this effect is equalized over time by aging; bone stiffness was smaller in the ovariectomized group and reduction of bone mass occurred. CONCLUSION: The removal of the ovaries produced systemic alterations, characterized by metabolic changes that caused weight gain and changes in bone tissue, associated with alteration of the mechanical profile and reduced bone mass. Level of Evidence I, Clinical Study. Sociedade Brasileira de Ortopedia e Traumatologia 2014 /pmc/articles/PMC4199643/ /pubmed/25328434 http://dx.doi.org/10.1590/1413-78522014220500420 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Bordinhon, Maristela Müller, Sérgio Swain Silva, Maeli Dal Pai Clinical, biomechanical and histological study on oophorectomy induced menopause |
title | Clinical, biomechanical and histological study on oophorectomy induced menopause |
title_full | Clinical, biomechanical and histological study on oophorectomy induced menopause |
title_fullStr | Clinical, biomechanical and histological study on oophorectomy induced menopause |
title_full_unstemmed | Clinical, biomechanical and histological study on oophorectomy induced menopause |
title_short | Clinical, biomechanical and histological study on oophorectomy induced menopause |
title_sort | clinical, biomechanical and histological study on oophorectomy induced menopause |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199643/ https://www.ncbi.nlm.nih.gov/pubmed/25328434 http://dx.doi.org/10.1590/1413-78522014220500420 |
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