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Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures
BACKGROUND: Sinopodophyllum hexandrum is an endangered medicinal herb, which is commonly present in elevations ranging between 2,400–4,500 m and is sensitive to temperature. Medicinal property of the species is attributed to the presence of podophyllotoxin in the rhizome tissue. The present work ana...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200142/ https://www.ncbi.nlm.nih.gov/pubmed/25287271 http://dx.doi.org/10.1186/1471-2164-15-871 |
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author | Kumari, Anita Singh, Heikham Russiachand Jha, Ashwani Swarnkar, Mohit Kumar Shankar, Ravi Kumar, Sanjay |
author_facet | Kumari, Anita Singh, Heikham Russiachand Jha, Ashwani Swarnkar, Mohit Kumar Shankar, Ravi Kumar, Sanjay |
author_sort | Kumari, Anita |
collection | PubMed |
description | BACKGROUND: Sinopodophyllum hexandrum is an endangered medicinal herb, which is commonly present in elevations ranging between 2,400–4,500 m and is sensitive to temperature. Medicinal property of the species is attributed to the presence of podophyllotoxin in the rhizome tissue. The present work analyzed transcriptome of rhizome tissue of S. hexandrum exposed to 15°C and 25°C to understand the temperature mediated molecular responses including those associated with podophyllotoxin biosynthesis. RESULTS: Deep sequencing of transcriptome with an average coverage of 88.34X yielded 60,089 assembled transcript sequences representing 20,387 unique genes having homology to known genes. Fragments per kilobase of exon per million fragments mapped (FPKM) based expression analysis revealed genes related to growth and development were over-expressed at 15°C, whereas genes involved in stress response were over-expressed at 25°C. There was a decreasing trend of podophyllotoxin accumulation at 25°C; data was well supported by the expression of corresponding genes of the pathway. FPKM data was validated by quantitative real-time polymerase chain reaction data using a total of thirty four genes and a positive correlation between the two platforms of gene expression was obtained. Also, detailed analyses yielded cytochrome P450s, methyltransferases and glycosyltransferases which could be the potential candidate hitherto unidentified genes of podophyllotoxin biosynthesis pathway. CONCLUSIONS: The present work revealed temperature responsive transcriptome of S. hexandrum on Illumina platform. Data suggested expression of genes for growth and development and podophyllotoxin biosynthesis at 15°C, and prevalence of those associated with stress response at 25°C. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-871) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4200142 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-42001422014-10-18 Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures Kumari, Anita Singh, Heikham Russiachand Jha, Ashwani Swarnkar, Mohit Kumar Shankar, Ravi Kumar, Sanjay BMC Genomics Research Article BACKGROUND: Sinopodophyllum hexandrum is an endangered medicinal herb, which is commonly present in elevations ranging between 2,400–4,500 m and is sensitive to temperature. Medicinal property of the species is attributed to the presence of podophyllotoxin in the rhizome tissue. The present work analyzed transcriptome of rhizome tissue of S. hexandrum exposed to 15°C and 25°C to understand the temperature mediated molecular responses including those associated with podophyllotoxin biosynthesis. RESULTS: Deep sequencing of transcriptome with an average coverage of 88.34X yielded 60,089 assembled transcript sequences representing 20,387 unique genes having homology to known genes. Fragments per kilobase of exon per million fragments mapped (FPKM) based expression analysis revealed genes related to growth and development were over-expressed at 15°C, whereas genes involved in stress response were over-expressed at 25°C. There was a decreasing trend of podophyllotoxin accumulation at 25°C; data was well supported by the expression of corresponding genes of the pathway. FPKM data was validated by quantitative real-time polymerase chain reaction data using a total of thirty four genes and a positive correlation between the two platforms of gene expression was obtained. Also, detailed analyses yielded cytochrome P450s, methyltransferases and glycosyltransferases which could be the potential candidate hitherto unidentified genes of podophyllotoxin biosynthesis pathway. CONCLUSIONS: The present work revealed temperature responsive transcriptome of S. hexandrum on Illumina platform. Data suggested expression of genes for growth and development and podophyllotoxin biosynthesis at 15°C, and prevalence of those associated with stress response at 25°C. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/1471-2164-15-871) contains supplementary material, which is available to authorized users. BioMed Central 2014-10-07 /pmc/articles/PMC4200142/ /pubmed/25287271 http://dx.doi.org/10.1186/1471-2164-15-871 Text en © Kumari et al.; licensee BioMed Central Ltd. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Kumari, Anita Singh, Heikham Russiachand Jha, Ashwani Swarnkar, Mohit Kumar Shankar, Ravi Kumar, Sanjay Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures |
title | Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures |
title_full | Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures |
title_fullStr | Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures |
title_full_unstemmed | Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures |
title_short | Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures |
title_sort | transcriptome sequencing of rhizome tissue of sinopodophyllum hexandrum at two temperatures |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200142/ https://www.ncbi.nlm.nih.gov/pubmed/25287271 http://dx.doi.org/10.1186/1471-2164-15-871 |
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