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Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes☆

The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 µM arseni...

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Autores principales: Wang, Yan, Zhao, Fenghong, Liao, Yingjun, Jin, Yaping, Sun, Guifan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200718/
https://www.ncbi.nlm.nih.gov/pubmed/25337094
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.31.005
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author Wang, Yan
Zhao, Fenghong
Liao, Yingjun
Jin, Yaping
Sun, Guifan
author_facet Wang, Yan
Zhao, Fenghong
Liao, Yingjun
Jin, Yaping
Sun, Guifan
author_sort Wang, Yan
collection PubMed
description The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 µM arsenite for 24 hours. Cell viability and morphological observations revealed that 5 µM arsenic exposure could induce cytotoxicity. Cells were then cultured in the presence of 0, 2.5, 5, or 10 µM arsenite for 24 hours and stimulated with 25 µM glutamate for 10 minutes. Results showed that [Ca(2+)](i) in astrocytes exposed to 5 and 10 µM arsenite was significantly increased and levels of D-serine, γ-aminobutyric acid and glycine in cultures exposed to 2.5–10 µM arsenite were also increased. However, glutamate levels in the media were significantly increased only after treatment with 10 µM arsenite. In conclusion, our findings suggest that arsenic exposure may affect glutamate-induced gliotransmitter release from astrocytes and further disturb neuronal function.
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spelling pubmed-42007182014-10-21 Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes☆ Wang, Yan Zhao, Fenghong Liao, Yingjun Jin, Yaping Sun, Guifan Neural Regen Res Research and Report Article: Brain Injury and Neural Regeneration The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 µM arsenite for 24 hours. Cell viability and morphological observations revealed that 5 µM arsenic exposure could induce cytotoxicity. Cells were then cultured in the presence of 0, 2.5, 5, or 10 µM arsenite for 24 hours and stimulated with 25 µM glutamate for 10 minutes. Results showed that [Ca(2+)](i) in astrocytes exposed to 5 and 10 µM arsenite was significantly increased and levels of D-serine, γ-aminobutyric acid and glycine in cultures exposed to 2.5–10 µM arsenite were also increased. However, glutamate levels in the media were significantly increased only after treatment with 10 µM arsenite. In conclusion, our findings suggest that arsenic exposure may affect glutamate-induced gliotransmitter release from astrocytes and further disturb neuronal function. Medknow Publications & Media Pvt Ltd 2012-11-05 /pmc/articles/PMC4200718/ /pubmed/25337094 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.31.005 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report Article: Brain Injury and Neural Regeneration
Wang, Yan
Zhao, Fenghong
Liao, Yingjun
Jin, Yaping
Sun, Guifan
Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes☆
title Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes☆
title_full Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes☆
title_fullStr Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes☆
title_full_unstemmed Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes☆
title_short Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes☆
title_sort arsenic exposure and glutamate-induced gliotransmitter release from astrocytes☆
topic Research and Report Article: Brain Injury and Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200718/
https://www.ncbi.nlm.nih.gov/pubmed/25337094
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.31.005
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