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Endophytic Fungi from Lycium chinense Mill and Characterization of Two New Korean Records of Colletotrichum

Chinese boxthorn or matrimony vine (Lycium chinense Mill) is found primarily in southeastern Europe and Asia, including Korea. The dried ripe fruits are commonly used as oriental medicinal purposes. Endophytic fungi were isolated from surface sterilized tissues and fruits of the medicinal plant in 2...

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Autores principales: Paul, Narayan Chandra, Lee, Hyang Burm, Lee, Ji Hye, Shin, Kyu Seop, Ryu, Tae Hee, Kwon, Hye Ri, Kim, Yeong Kuk, Youn, Young Nam, Yu, Seung Hun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200795/
https://www.ncbi.nlm.nih.gov/pubmed/25170812
http://dx.doi.org/10.3390/ijms150915272
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author Paul, Narayan Chandra
Lee, Hyang Burm
Lee, Ji Hye
Shin, Kyu Seop
Ryu, Tae Hee
Kwon, Hye Ri
Kim, Yeong Kuk
Youn, Young Nam
Yu, Seung Hun
author_facet Paul, Narayan Chandra
Lee, Hyang Burm
Lee, Ji Hye
Shin, Kyu Seop
Ryu, Tae Hee
Kwon, Hye Ri
Kim, Yeong Kuk
Youn, Young Nam
Yu, Seung Hun
author_sort Paul, Narayan Chandra
collection PubMed
description Chinese boxthorn or matrimony vine (Lycium chinense Mill) is found primarily in southeastern Europe and Asia, including Korea. The dried ripe fruits are commonly used as oriental medicinal purposes. Endophytic fungi were isolated from surface sterilized tissues and fruits of the medicinal plant in 2013 to identify the new or unreported species in Korea. Among 14 isolates, 10 morphospecies were selected for molecular identification with the internal transcribed spacer (ITS) gene. Phylogenetic analysis revealed that all isolates belonged to Ascomycota including the genera Acremonium, Colletotrichum, Cochliobolus, Fusarium, Hypocrea and Nemania. Two Colletotrichum species were identified at the species level, using three genes including internal transcribed spacer (ITS), glycerol-3-phosphate dehydrogenase (GAPDH) and Actin (ACT) for PCR and molecular data analysis along with morphological observations. The fungal isolates, CNU122031 and CNU122032 were identified as Colletotrichum fructicola and C. brevisporum, respectively. Morphological observations also well supported the molecular identification. C. brevisporum is represented unrecorded species in Korea and C. fructicola is the first record from the host plant.
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spelling pubmed-42007952014-10-17 Endophytic Fungi from Lycium chinense Mill and Characterization of Two New Korean Records of Colletotrichum Paul, Narayan Chandra Lee, Hyang Burm Lee, Ji Hye Shin, Kyu Seop Ryu, Tae Hee Kwon, Hye Ri Kim, Yeong Kuk Youn, Young Nam Yu, Seung Hun Int J Mol Sci Article Chinese boxthorn or matrimony vine (Lycium chinense Mill) is found primarily in southeastern Europe and Asia, including Korea. The dried ripe fruits are commonly used as oriental medicinal purposes. Endophytic fungi were isolated from surface sterilized tissues and fruits of the medicinal plant in 2013 to identify the new or unreported species in Korea. Among 14 isolates, 10 morphospecies were selected for molecular identification with the internal transcribed spacer (ITS) gene. Phylogenetic analysis revealed that all isolates belonged to Ascomycota including the genera Acremonium, Colletotrichum, Cochliobolus, Fusarium, Hypocrea and Nemania. Two Colletotrichum species were identified at the species level, using three genes including internal transcribed spacer (ITS), glycerol-3-phosphate dehydrogenase (GAPDH) and Actin (ACT) for PCR and molecular data analysis along with morphological observations. The fungal isolates, CNU122031 and CNU122032 were identified as Colletotrichum fructicola and C. brevisporum, respectively. Morphological observations also well supported the molecular identification. C. brevisporum is represented unrecorded species in Korea and C. fructicola is the first record from the host plant. MDPI 2014-08-28 /pmc/articles/PMC4200795/ /pubmed/25170812 http://dx.doi.org/10.3390/ijms150915272 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Paul, Narayan Chandra
Lee, Hyang Burm
Lee, Ji Hye
Shin, Kyu Seop
Ryu, Tae Hee
Kwon, Hye Ri
Kim, Yeong Kuk
Youn, Young Nam
Yu, Seung Hun
Endophytic Fungi from Lycium chinense Mill and Characterization of Two New Korean Records of Colletotrichum
title Endophytic Fungi from Lycium chinense Mill and Characterization of Two New Korean Records of Colletotrichum
title_full Endophytic Fungi from Lycium chinense Mill and Characterization of Two New Korean Records of Colletotrichum
title_fullStr Endophytic Fungi from Lycium chinense Mill and Characterization of Two New Korean Records of Colletotrichum
title_full_unstemmed Endophytic Fungi from Lycium chinense Mill and Characterization of Two New Korean Records of Colletotrichum
title_short Endophytic Fungi from Lycium chinense Mill and Characterization of Two New Korean Records of Colletotrichum
title_sort endophytic fungi from lycium chinense mill and characterization of two new korean records of colletotrichum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4200795/
https://www.ncbi.nlm.nih.gov/pubmed/25170812
http://dx.doi.org/10.3390/ijms150915272
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