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Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle,
OBJECTIVE: To evaluate oxidative damage (lipid oxidation, protein oxidation, thiobarbituric acid-reactive substances [TBARS], and carbonylation) and inflammation (expression of phosphorylated AMP-activated protein kinase and mammalian target of rapamycin [p-AMPK and p-mTOR, respectively]) in the lun...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Pneumologia e Tisiologia
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4201172/ https://www.ncbi.nlm.nih.gov/pubmed/25210964 http://dx.doi.org/10.1590/S1806-37132014000400009 |
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author | de Carlos, Samanta Portão Dias, Alexandre Simões Forgiarini, Luiz Alberto Patricio, Patrícia Damiani Graciano, Thaise Nesi, Renata Tiscoski Valença, Samuel Chiappa, Adriana Meira Guntzel Cipriano, Gerson de Souza, Claudio Teodoro Chiappa, Gaspar Rogério da Silva |
author_facet | de Carlos, Samanta Portão Dias, Alexandre Simões Forgiarini, Luiz Alberto Patricio, Patrícia Damiani Graciano, Thaise Nesi, Renata Tiscoski Valença, Samuel Chiappa, Adriana Meira Guntzel Cipriano, Gerson de Souza, Claudio Teodoro Chiappa, Gaspar Rogério da Silva |
author_sort | de Carlos, Samanta Portão |
collection | PubMed |
description | OBJECTIVE: To evaluate oxidative damage (lipid oxidation, protein oxidation, thiobarbituric acid-reactive substances [TBARS], and carbonylation) and inflammation (expression of phosphorylated AMP-activated protein kinase and mammalian target of rapamycin [p-AMPK and p-mTOR, respectively]) in the lung parenchyma and diaphragm muscles of male C57BL-6 mice exposed to cigarette smoke (CS) for 7, 15, 30, 45, or 60 days. METHODS: Thirty-six male C57BL-6 mice were divided into six groups (n = 6/group): a control group; and five groups exposed to CS for 7, 15, 30, 45, and 60 days, respectively. RESULTS: Compared with control mice, CS-exposed mice presented lower body weights at 30 days. In CS-exposed mice (compared with control mice), the greatest differences (increases) in TBARS levels were observed on day 7 in diaphragm-muscle, compared with day 45 in lung tissue; the greatest differences (increases) in carbonyl levels were observed on day 7 in both tissue types; and sulfhydryl levels were lower, in both tissue types, at all time points. In lung tissue and diaphragm muscle, p-AMPK expression exhibited behavior similar to that of TBARS. Expression of p-mTOR was higher than the control value on days 7 and 15 in lung tissue, as it was on day 45 in diaphragm muscle. CONCLUSION: Our data demonstrate that CS exposure produces oxidative damage, not only in lung tissue but also (primarily) in muscle tissue, having an additional effect on respiratory muscle, as is frequently observed in smokers with COPD. |
format | Online Article Text |
id | pubmed-4201172 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Sociedade Brasileira de Pneumologia e Tisiologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-42011722014-10-21 Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle, de Carlos, Samanta Portão Dias, Alexandre Simões Forgiarini, Luiz Alberto Patricio, Patrícia Damiani Graciano, Thaise Nesi, Renata Tiscoski Valença, Samuel Chiappa, Adriana Meira Guntzel Cipriano, Gerson de Souza, Claudio Teodoro Chiappa, Gaspar Rogério da Silva J Bras Pneumol Original Articles OBJECTIVE: To evaluate oxidative damage (lipid oxidation, protein oxidation, thiobarbituric acid-reactive substances [TBARS], and carbonylation) and inflammation (expression of phosphorylated AMP-activated protein kinase and mammalian target of rapamycin [p-AMPK and p-mTOR, respectively]) in the lung parenchyma and diaphragm muscles of male C57BL-6 mice exposed to cigarette smoke (CS) for 7, 15, 30, 45, or 60 days. METHODS: Thirty-six male C57BL-6 mice were divided into six groups (n = 6/group): a control group; and five groups exposed to CS for 7, 15, 30, 45, and 60 days, respectively. RESULTS: Compared with control mice, CS-exposed mice presented lower body weights at 30 days. In CS-exposed mice (compared with control mice), the greatest differences (increases) in TBARS levels were observed on day 7 in diaphragm-muscle, compared with day 45 in lung tissue; the greatest differences (increases) in carbonyl levels were observed on day 7 in both tissue types; and sulfhydryl levels were lower, in both tissue types, at all time points. In lung tissue and diaphragm muscle, p-AMPK expression exhibited behavior similar to that of TBARS. Expression of p-mTOR was higher than the control value on days 7 and 15 in lung tissue, as it was on day 45 in diaphragm muscle. CONCLUSION: Our data demonstrate that CS exposure produces oxidative damage, not only in lung tissue but also (primarily) in muscle tissue, having an additional effect on respiratory muscle, as is frequently observed in smokers with COPD. Sociedade Brasileira de Pneumologia e Tisiologia 2014 /pmc/articles/PMC4201172/ /pubmed/25210964 http://dx.doi.org/10.1590/S1806-37132014000400009 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles de Carlos, Samanta Portão Dias, Alexandre Simões Forgiarini, Luiz Alberto Patricio, Patrícia Damiani Graciano, Thaise Nesi, Renata Tiscoski Valença, Samuel Chiappa, Adriana Meira Guntzel Cipriano, Gerson de Souza, Claudio Teodoro Chiappa, Gaspar Rogério da Silva Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle, |
title | Oxidative damage induced by cigarette smoke exposure in mice: impact on
lung tissue and diaphragm muscle,
|
title_full | Oxidative damage induced by cigarette smoke exposure in mice: impact on
lung tissue and diaphragm muscle,
|
title_fullStr | Oxidative damage induced by cigarette smoke exposure in mice: impact on
lung tissue and diaphragm muscle,
|
title_full_unstemmed | Oxidative damage induced by cigarette smoke exposure in mice: impact on
lung tissue and diaphragm muscle,
|
title_short | Oxidative damage induced by cigarette smoke exposure in mice: impact on
lung tissue and diaphragm muscle,
|
title_sort | oxidative damage induced by cigarette smoke exposure in mice: impact on
lung tissue and diaphragm muscle, |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4201172/ https://www.ncbi.nlm.nih.gov/pubmed/25210964 http://dx.doi.org/10.1590/S1806-37132014000400009 |
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