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Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle,

OBJECTIVE: To evaluate oxidative damage (lipid oxidation, protein oxidation, thiobarbituric acid-reactive substances [TBARS], and carbonylation) and inflammation (expression of phosphorylated AMP-activated protein kinase and mammalian target of rapamycin [p-AMPK and p-mTOR, respectively]) in the lun...

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Autores principales: de Carlos, Samanta Portão, Dias, Alexandre Simões, Forgiarini, Luiz Alberto, Patricio, Patrícia Damiani, Graciano, Thaise, Nesi, Renata Tiscoski, Valença, Samuel, Chiappa, Adriana Meira Guntzel, Cipriano, Gerson, de Souza, Claudio Teodoro, Chiappa, Gaspar Rogério da Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Pneumologia e Tisiologia 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4201172/
https://www.ncbi.nlm.nih.gov/pubmed/25210964
http://dx.doi.org/10.1590/S1806-37132014000400009
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author de Carlos, Samanta Portão
Dias, Alexandre Simões
Forgiarini, Luiz Alberto
Patricio, Patrícia Damiani
Graciano, Thaise
Nesi, Renata Tiscoski
Valença, Samuel
Chiappa, Adriana Meira Guntzel
Cipriano, Gerson
de Souza, Claudio Teodoro
Chiappa, Gaspar Rogério da Silva
author_facet de Carlos, Samanta Portão
Dias, Alexandre Simões
Forgiarini, Luiz Alberto
Patricio, Patrícia Damiani
Graciano, Thaise
Nesi, Renata Tiscoski
Valença, Samuel
Chiappa, Adriana Meira Guntzel
Cipriano, Gerson
de Souza, Claudio Teodoro
Chiappa, Gaspar Rogério da Silva
author_sort de Carlos, Samanta Portão
collection PubMed
description OBJECTIVE: To evaluate oxidative damage (lipid oxidation, protein oxidation, thiobarbituric acid-reactive substances [TBARS], and carbonylation) and inflammation (expression of phosphorylated AMP-activated protein kinase and mammalian target of rapamycin [p-AMPK and p-mTOR, respectively]) in the lung parenchyma and diaphragm muscles of male C57BL-6 mice exposed to cigarette smoke (CS) for 7, 15, 30, 45, or 60 days. METHODS: Thirty-six male C57BL-6 mice were divided into six groups (n = 6/group): a control group; and five groups exposed to CS for 7, 15, 30, 45, and 60 days, respectively. RESULTS: Compared with control mice, CS-exposed mice presented lower body weights at 30 days. In CS-exposed mice (compared with control mice), the greatest differences (increases) in TBARS levels were observed on day 7 in diaphragm-muscle, compared with day 45 in lung tissue; the greatest differences (increases) in carbonyl levels were observed on day 7 in both tissue types; and sulfhydryl levels were lower, in both tissue types, at all time points. In lung tissue and diaphragm muscle, p-AMPK expression exhibited behavior similar to that of TBARS. Expression of p-mTOR was higher than the control value on days 7 and 15 in lung tissue, as it was on day 45 in diaphragm muscle. CONCLUSION: Our data demonstrate that CS exposure produces oxidative damage, not only in lung tissue but also (primarily) in muscle tissue, having an additional effect on respiratory muscle, as is frequently observed in smokers with COPD.
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spelling pubmed-42011722014-10-21 Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle, de Carlos, Samanta Portão Dias, Alexandre Simões Forgiarini, Luiz Alberto Patricio, Patrícia Damiani Graciano, Thaise Nesi, Renata Tiscoski Valença, Samuel Chiappa, Adriana Meira Guntzel Cipriano, Gerson de Souza, Claudio Teodoro Chiappa, Gaspar Rogério da Silva J Bras Pneumol Original Articles OBJECTIVE: To evaluate oxidative damage (lipid oxidation, protein oxidation, thiobarbituric acid-reactive substances [TBARS], and carbonylation) and inflammation (expression of phosphorylated AMP-activated protein kinase and mammalian target of rapamycin [p-AMPK and p-mTOR, respectively]) in the lung parenchyma and diaphragm muscles of male C57BL-6 mice exposed to cigarette smoke (CS) for 7, 15, 30, 45, or 60 days. METHODS: Thirty-six male C57BL-6 mice were divided into six groups (n = 6/group): a control group; and five groups exposed to CS for 7, 15, 30, 45, and 60 days, respectively. RESULTS: Compared with control mice, CS-exposed mice presented lower body weights at 30 days. In CS-exposed mice (compared with control mice), the greatest differences (increases) in TBARS levels were observed on day 7 in diaphragm-muscle, compared with day 45 in lung tissue; the greatest differences (increases) in carbonyl levels were observed on day 7 in both tissue types; and sulfhydryl levels were lower, in both tissue types, at all time points. In lung tissue and diaphragm muscle, p-AMPK expression exhibited behavior similar to that of TBARS. Expression of p-mTOR was higher than the control value on days 7 and 15 in lung tissue, as it was on day 45 in diaphragm muscle. CONCLUSION: Our data demonstrate that CS exposure produces oxidative damage, not only in lung tissue but also (primarily) in muscle tissue, having an additional effect on respiratory muscle, as is frequently observed in smokers with COPD. Sociedade Brasileira de Pneumologia e Tisiologia 2014 /pmc/articles/PMC4201172/ /pubmed/25210964 http://dx.doi.org/10.1590/S1806-37132014000400009 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
de Carlos, Samanta Portão
Dias, Alexandre Simões
Forgiarini, Luiz Alberto
Patricio, Patrícia Damiani
Graciano, Thaise
Nesi, Renata Tiscoski
Valença, Samuel
Chiappa, Adriana Meira Guntzel
Cipriano, Gerson
de Souza, Claudio Teodoro
Chiappa, Gaspar Rogério da Silva
Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle,
title Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle,
title_full Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle,
title_fullStr Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle,
title_full_unstemmed Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle,
title_short Oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle,
title_sort oxidative damage induced by cigarette smoke exposure in mice: impact on lung tissue and diaphragm muscle,
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4201172/
https://www.ncbi.nlm.nih.gov/pubmed/25210964
http://dx.doi.org/10.1590/S1806-37132014000400009
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