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An Unbiased Approach To Identify Endogenous Substrates of “Histone” Deacetylase 8
[Image: see text] Despite being extensively characterized structurally and biochemically, the functional role of histone deacetylase 8 (HDAC8) has remained largely obscure due in part to a lack of known cellular substrates. Herein, we describe an unbiased approach using chemical tools in conjunction...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical
Society
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4201337/ https://www.ncbi.nlm.nih.gov/pubmed/25089360 http://dx.doi.org/10.1021/cb500492r |
| _version_ | 1782340162070511616 |
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| author | Olson, David E. Udeshi, Namrata D. Wolfson, Noah A. Pitcairn, Carol Ann Sullivan, Eric D. Jaffe, Jacob D. Svinkina, Tanya Natoli, Ted Lu, Xiaodong Paulk, Joshiawa McCarren, Patrick Wagner, Florence F. Barker, Doug Howe, Eleanor Lazzaro, Fanny Gale, Jennifer P. Zhang, Yan-Ling Subramanian, Aravind Fierke, Carol A. Carr, Steven A. Holson, Edward B. |
| author_facet | Olson, David E. Udeshi, Namrata D. Wolfson, Noah A. Pitcairn, Carol Ann Sullivan, Eric D. Jaffe, Jacob D. Svinkina, Tanya Natoli, Ted Lu, Xiaodong Paulk, Joshiawa McCarren, Patrick Wagner, Florence F. Barker, Doug Howe, Eleanor Lazzaro, Fanny Gale, Jennifer P. Zhang, Yan-Ling Subramanian, Aravind Fierke, Carol A. Carr, Steven A. Holson, Edward B. |
| author_sort | Olson, David E. |
| collection | PubMed |
| description | [Image: see text] Despite being extensively characterized structurally and biochemically, the functional role of histone deacetylase 8 (HDAC8) has remained largely obscure due in part to a lack of known cellular substrates. Herein, we describe an unbiased approach using chemical tools in conjunction with sophisticated proteomics methods to identify novel non-histone nuclear substrates of HDAC8, including the tumor suppressor ARID1A. These newly discovered substrates of HDAC8 are involved in diverse biological processes including mitosis, transcription, chromatin remodeling, and RNA splicing and may help guide therapeutic strategies that target the function of HDAC8. |
| format | Online Article Text |
| id | pubmed-4201337 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2014 |
| publisher | American Chemical
Society |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-42013372015-08-04 An Unbiased Approach To Identify Endogenous Substrates of “Histone” Deacetylase 8 Olson, David E. Udeshi, Namrata D. Wolfson, Noah A. Pitcairn, Carol Ann Sullivan, Eric D. Jaffe, Jacob D. Svinkina, Tanya Natoli, Ted Lu, Xiaodong Paulk, Joshiawa McCarren, Patrick Wagner, Florence F. Barker, Doug Howe, Eleanor Lazzaro, Fanny Gale, Jennifer P. Zhang, Yan-Ling Subramanian, Aravind Fierke, Carol A. Carr, Steven A. Holson, Edward B. ACS Chem Biol [Image: see text] Despite being extensively characterized structurally and biochemically, the functional role of histone deacetylase 8 (HDAC8) has remained largely obscure due in part to a lack of known cellular substrates. Herein, we describe an unbiased approach using chemical tools in conjunction with sophisticated proteomics methods to identify novel non-histone nuclear substrates of HDAC8, including the tumor suppressor ARID1A. These newly discovered substrates of HDAC8 are involved in diverse biological processes including mitosis, transcription, chromatin remodeling, and RNA splicing and may help guide therapeutic strategies that target the function of HDAC8. American Chemical Society 2014-08-04 2014-10-17 /pmc/articles/PMC4201337/ /pubmed/25089360 http://dx.doi.org/10.1021/cb500492r Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) |
| spellingShingle | Olson, David E. Udeshi, Namrata D. Wolfson, Noah A. Pitcairn, Carol Ann Sullivan, Eric D. Jaffe, Jacob D. Svinkina, Tanya Natoli, Ted Lu, Xiaodong Paulk, Joshiawa McCarren, Patrick Wagner, Florence F. Barker, Doug Howe, Eleanor Lazzaro, Fanny Gale, Jennifer P. Zhang, Yan-Ling Subramanian, Aravind Fierke, Carol A. Carr, Steven A. Holson, Edward B. An Unbiased Approach To Identify Endogenous Substrates of “Histone” Deacetylase 8 |
| title | An Unbiased Approach To Identify Endogenous Substrates
of “Histone” Deacetylase 8 |
| title_full | An Unbiased Approach To Identify Endogenous Substrates
of “Histone” Deacetylase 8 |
| title_fullStr | An Unbiased Approach To Identify Endogenous Substrates
of “Histone” Deacetylase 8 |
| title_full_unstemmed | An Unbiased Approach To Identify Endogenous Substrates
of “Histone” Deacetylase 8 |
| title_short | An Unbiased Approach To Identify Endogenous Substrates
of “Histone” Deacetylase 8 |
| title_sort | unbiased approach to identify endogenous substrates
of “histone” deacetylase 8 |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4201337/ https://www.ncbi.nlm.nih.gov/pubmed/25089360 http://dx.doi.org/10.1021/cb500492r |
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