How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry

Radiation of experimental culture cells on plates with various wells can cause a risk of underdosage as a result of the existence of multiple air–water interfaces. The objective of our study was to quantify this error in culture plates with multiple wells. Radiation conditions were simulated with th...

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Autores principales: Sabater, Sebastia, Berenguer, Roberto, Honrubia-Gomez, Paloma, Rivera, Miguel, Nuñez, Ana, Jimenez-Jimenez, Esther, Martos, Ana, Ramirez-Castillejo, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Technology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202281/
https://www.ncbi.nlm.nih.gov/pubmed/24722683
http://dx.doi.org/10.1093/jrr/rru022
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author Sabater, Sebastia
Berenguer, Roberto
Honrubia-Gomez, Paloma
Rivera, Miguel
Nuñez, Ana
Jimenez-Jimenez, Esther
Martos, Ana
Ramirez-Castillejo, Carmen
author_facet Sabater, Sebastia
Berenguer, Roberto
Honrubia-Gomez, Paloma
Rivera, Miguel
Nuñez, Ana
Jimenez-Jimenez, Esther
Martos, Ana
Ramirez-Castillejo, Carmen
author_sort Sabater, Sebastia
collection PubMed
description Radiation of experimental culture cells on plates with various wells can cause a risk of underdosage as a result of the existence of multiple air–water interfaces. The objective of our study was to quantify this error in culture plates with multiple wells. Radiation conditions were simulated with the GAMOS code, based on the GEANT4 code, and this was compared with a simulation performed with PENELOPE and measured data. We observed a slight underdosage of ∼4% on the most superficial half of the culture medium. We believe that this underdosage does not have a significant effect on the dose received by culture cells deposited in a monolayer and adhered to the base of the wells.
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spelling pubmed-42022812014-10-23 How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry Sabater, Sebastia Berenguer, Roberto Honrubia-Gomez, Paloma Rivera, Miguel Nuñez, Ana Jimenez-Jimenez, Esther Martos, Ana Ramirez-Castillejo, Carmen J Radiat Res Technology Radiation of experimental culture cells on plates with various wells can cause a risk of underdosage as a result of the existence of multiple air–water interfaces. The objective of our study was to quantify this error in culture plates with multiple wells. Radiation conditions were simulated with the GAMOS code, based on the GEANT4 code, and this was compared with a simulation performed with PENELOPE and measured data. We observed a slight underdosage of ∼4% on the most superficial half of the culture medium. We believe that this underdosage does not have a significant effect on the dose received by culture cells deposited in a monolayer and adhered to the base of the wells. Oxford University Press 2014-09 2014-04-10 /pmc/articles/PMC4202281/ /pubmed/24722683 http://dx.doi.org/10.1093/jrr/rru022 Text en © The Author 2014. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technology
Sabater, Sebastia
Berenguer, Roberto
Honrubia-Gomez, Paloma
Rivera, Miguel
Nuñez, Ana
Jimenez-Jimenez, Esther
Martos, Ana
Ramirez-Castillejo, Carmen
How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry
title How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry
title_full How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry
title_fullStr How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry
title_full_unstemmed How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry
title_short How air influences radiation dose deposition in multiwell culture plates: a Monte Carlo simulation of radiation geometry
title_sort how air influences radiation dose deposition in multiwell culture plates: a monte carlo simulation of radiation geometry
topic Technology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4202281/
https://www.ncbi.nlm.nih.gov/pubmed/24722683
http://dx.doi.org/10.1093/jrr/rru022
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